PMID- 36245825
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221019
IS  - 1792-1082 (Electronic)
IS  - 1792-1074 (Print)
IS  - 1792-1074 (Linking)
VI  - 24
IP  - 5
DP  - 2022 Nov
TI  - Differentially expressed genes related to lymph node metastasis in advanced 
      laryngeal squamous cell cancers.
PG  - 409
LID - 10.3892/ol.2022.13529 [doi]
LID - 409
AB  - Understanding the molecular mechanisms and gene expression in laryngeal squamous 
      cell carcinoma (LSCC) may explain its aggressive biological behavior and regional 
      metastasis pathways. In the present study, patients with locally advanced LSCC 
      tumors were examined for differential gene expression in the normal mucosa 
      (non-tumoral mucosa), tumors and lymph node tissues. The aim was to identify 
      possible predictive genes for lymph node metastasis. A total of 16 patients who 
      had undergone total laryngectomy with neck dissection for advanced LSCC were 
      randomly selected from a hospital database: Eight of the patients had lymph node 
      metastasis (Group 1) and the other eight patients did not have metastasis (Group 
      2). Overall survival (OS), disease-free survival (DFS) and disease-specific 
      survival (DSS) were analyzed. For each patient, paraffin-embedded tissue samples 
      were collected from non-tumoral mucosa, tumoral lesions and lymph node tissues. 
      RNA was extracted from the tissue samples and used for complementary DNA 
      synthesis, and microarray analysis was subsequently performed on each sample. 
      Gene expression levels were determined in each specimen, and Groups 1 and 2 were 
      compared and statistically analyzed. The microarray results for lymph node 
      metastasis-positive and -negative groups, indicated the differential expression 
      of 312 genes in the lymph nodes, 691 genes in the normal mucosal tissue and 93 
      genes in the tumor tissue. Transgelin (TAGLN) and cofilin 1 (CFL1) were 
      identified as possible target genes and validated using reverse 
      transcription-quantitative polymerase chain reaction (RT-qPCR). The RT-qPCR 
      results for TAGLN and CFL1 supported the microarray data. OS, DFS and DSS times 
      were longer in Group 2 than in Group 1 (P=0.002, 0.015 and 0.009, respectively). 
      In addition, TAGLN and CFL1 were associated with DFS and DSS. On the basis of 
      these results, it is suggested that TAGLN and CFL1 expression may play an 
      important role in the pathogenesis of regional metastasis and poor prognosis in 
      advanced LSCC.
CI  - Copyright: (c) Bayir et al.
FAU - Bayir, Omer
AU  - Bayir O
AD  - Department of Otolaryngology, Head and Neck Surgery, University of Health 
      Sciences, Diskapi Yildirim Beyazit Training and Research Hospital, Ankara 06110, 
      Turkey.
FAU - Asik, Mehmet Dogan
AU  - Asik MD
AD  - Department of Medical Biology, School of Medicine, Ankara Yildirim Beyazit 
      University, Ankara 06010, Turkey.
FAU - Saylam, Guleser
AU  - Saylam G
AD  - Department of Otolaryngology, Head and Neck Surgery, University of Health 
      Sciences, Diskapi Yildirim Beyazit Training and Research Hospital, Ankara 06110, 
      Turkey.
FAU - Pinarli, Ferda Alpaslan
AU  - Pinarli FA
AD  - Myogen Laboratory and Health Services, Ankara 06520, Turkey.
FAU - Tatar, Emel Cadalli
AU  - Tatar EC
AD  - Department of Otolaryngology, Head and Neck Surgery, University of Health 
      Sciences, Diskapi Yildirim Beyazit Training and Research Hospital, Ankara 06110, 
      Turkey.
FAU - Han, Unsal
AU  - Han U
AD  - Department of Pathology, University of Health Sciences, Diskapi Yildirim Beyazit 
      Training and Research Hospital, Ankara 06110, Turkey.
FAU - Simsek, Ender
AU  - Simsek E
AD  - Department of Medical Biology, School of Medicine, Ankara Yildirim Beyazit 
      University, Ankara 06010, Turkey.
FAU - Korkmaz, Mehmet Hakan
AU  - Korkmaz MH
AD  - Department of Otolaryngology, Head and Neck Surgery, University of Health 
      Sciences, Diskapi Yildirim Beyazit Training and Research Hospital, Ankara 06110, 
      Turkey.
AD  - Department of Otolaryngology, Head and Neck Surgery, School of Medicine, Ankara 
      Yildirim Beyazit University, Ankara 06010, Turkey.
LA  - eng
PT  - Journal Article
DEP - 20220926
PL  - Greece
TA  - Oncol Lett
JT  - Oncology letters
JID - 101531236
PMC - PMC9555062
OTO - NOTNLM
OT  - cofilin-1
OT  - gene expression profile
OT  - laryngeal squamous cell cancer
OT  - microarray
OT  - tansgelin
COIS- The authors declare that they have no competing interests.
EDAT- 2022/10/18 06:00
MHDA- 2022/10/18 06:01
PMCR- 2022/09/26
CRDT- 2022/10/17 04:20
PHST- 2022/01/02 00:00 [received]
PHST- 2022/07/19 00:00 [accepted]
PHST- 2022/10/17 04:20 [entrez]
PHST- 2022/10/18 06:00 [pubmed]
PHST- 2022/10/18 06:01 [medline]
PHST- 2022/09/26 00:00 [pmc-release]
AID - OL-24-05-13529 [pii]
AID - 10.3892/ol.2022.13529 [doi]
PST - epublish
SO  - Oncol Lett. 2022 Sep 26;24(5):409. doi: 10.3892/ol.2022.13529. eCollection 2022 
      Nov.