PMID- 36246378
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221019
IS  - 2296-4185 (Print)
IS  - 2296-4185 (Electronic)
IS  - 2296-4185 (Linking)
VI  - 10
DP  - 2022
TI  - Chondrogenic differentiation of mesenchymal stem cells through cartilage 
      matrix-inspired surface coatings.
PG  - 991855
LID - 10.3389/fbioe.2022.991855 [doi]
LID - 991855
AB  - The stem cell niche comprises soluble molecules and extracellular matrix 
      components which provide chemical and mechanical cues that determine the 
      differentiation of stem cells. Here, the effect of polyelectrolyte multilayer 
      (PEM) composition and terminal layer fabricated with layer-by-layer technique 
      (LBL) pairing either hyaluronan [in its native (nHA) and oxidized form (oHA)] or 
      chondroitin sulfate (CS) with type I collagen (Col I) is investigated on 
      chondrogenic differentiation of human umbilical mesenchymal stem cells 
      (hUC-MSCs). Physical studies performed to investigate the establishment and 
      structure of the surface coatings show that PEM composed of HA and Col I show a 
      dominance of nHA or oHA with considerably lesser organization of Col I fibrils. 
      In contrast, distinguished fibrilized Col I is found in nCS-containing PEM. 
      Generally, Col I-terminated PEM promote the adhesion, migration, and growth of 
      hUC-MSCs more than GAG-terminated surfaces due to the presence of fibrillar Col I 
      but show a lower degree of differentiation towards the chondrogenic lineage. 
      Notably, the Col I/nHA PEM not only supports adhesion and growth of hUC-MSCs but 
      also significantly promotes cartilage-associated gene and protein expression as 
      found by histochemical and molecular biology studies, which is not seen on the 
      Col I/oHA PEM. This is related to ligation of HA to the cell receptor CD44 
      followed by activation of ERK/Sox9 and noncanonical TGF-beta signaling-p38 pathways 
      that depends on the molecular weight of HA as found by immune histochemical and 
      western blotting. Hence, surface coatings on scaffolds and other implants by PEM 
      composed of nHA and Col I may be useful for programming MSC towards cartilage 
      regeneration.
CI  - Copyright (c) 2022 Zhao, Gao, Wei, Tu, Zheng, Jing, Chu, Ye and Groth.
FAU - Zhao, Mingyan
AU  - Zhao M
AD  - Stem Cell Research and Cellular Therapy Center, Affiliated Hospital of Guangdong 
      Medical University, Zhanjiang, China.
FAU - Gao, Xiang
AU  - Gao X
AD  - Stem Cell Research and Cellular Therapy Center, Affiliated Hospital of Guangdong 
      Medical University, Zhanjiang, China.
FAU - Wei, Jinsong
AU  - Wei J
AD  - Department of Spinal Surgery, Affiliated Hospital of Guangdong Medical 
      University, Zhanjiang, China.
FAU - Tu, Chenlin
AU  - Tu C
AD  - Stem Cell Research and Cellular Therapy Center, Affiliated Hospital of Guangdong 
      Medical University, Zhanjiang, China.
AD  - Department of Spinal Surgery, Affiliated Hospital of Guangdong Medical 
      University, Zhanjiang, China.
FAU - Zheng, Hong
AU  - Zheng H
AD  - Department of Spinal Surgery, Affiliated Hospital of Guangdong Medical 
      University, Zhanjiang, China.
FAU - Jing, Kaipeng
AU  - Jing K
AD  - Key Laboratory of Prevention and Management of Chronic Kidney Disease of 
      Zhanjiang City, Institute of Nephrology, Affiliated Hospital of Guangdong Medical 
      University, Zhanjiang, China.
FAU - Chu, Jiaqi
AU  - Chu J
AD  - Stem Cell Research and Cellular Therapy Center, Affiliated Hospital of Guangdong 
      Medical University, Zhanjiang, China.
FAU - Ye, Wei
AU  - Ye W
AD  - Department of Obstetrics and Gynecology, Affiliated Hospital of Guangdong Medical 
      University, Zhanjiang, China.
FAU - Groth, Thomas
AU  - Groth T
AD  - Department Biomedical Materials, Institute of Pharmacy, Martin Luther University 
      Halle Wittenberg, Halle (Saale), Germany.
LA  - eng
PT  - Journal Article
DEP - 20220929
PL  - Switzerland
TA  - Front Bioeng Biotechnol
JT  - Frontiers in bioengineering and biotechnology
JID - 101632513
PMC - PMC9557131
OTO - NOTNLM
OT  - ERK/Sox9 pathway
OT  - biomimetic multilayers
OT  - chondrogenesis
OT  - microenvironment
OT  - noncanonical TGF-beta pathway
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/10/18 06:00
MHDA- 2022/10/18 06:01
PMCR- 2022/01/01
CRDT- 2022/10/17 04:30
PHST- 2022/07/12 00:00 [received]
PHST- 2022/09/14 00:00 [accepted]
PHST- 2022/10/17 04:30 [entrez]
PHST- 2022/10/18 06:00 [pubmed]
PHST- 2022/10/18 06:01 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 991855 [pii]
AID - 10.3389/fbioe.2022.991855 [doi]
PST - epublish
SO  - Front Bioeng Biotechnol. 2022 Sep 29;10:991855. doi: 10.3389/fbioe.2022.991855. 
      eCollection 2022.