PMID- 36247288
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221019
IS  - 1943-8141 (Print)
IS  - 1943-8141 (Electronic)
IS  - 1943-8141 (Linking)
VI  - 14
IP  - 9
DP  - 2022
TI  - Serum microRNAs as non-invasive diagnostic biomarkers for intrahepatic 
      cholestasis of pregnancy.
PG  - 6763-6773
AB  - OBJECTIVES: Intrahepatic cholestasis of pregnancy (IHCP) causes itching, preterm 
      birth, and stillbirth. However, there is no accurate diagnostic method for IHCP. 
      Currently, circulating microRNAs (miRNAs) have become candidate biomarkers for 
      the diagnosis of multiple diseases. Here, we investigated the diagnostic value of 
      miRNAs in IHCP and aimed to predict the molecular mechanism of IHCP pathogenesis. 
      METHODS: We analyzed differentially expressed miRNAs in both women with IHCP and 
      normal pregnant women. The selected candidate miRNAs were validated in 46 IHCP 
      cases and 46 normal pregnant subjects, and we constructed receiver operator 
      characteristic curves of miRNAs. Pearson correlations between levels of total 
      bile acid (TBA) and differentially expressed miRNAs were also calculated. In 
      addition, we clustered functionally significant biological pathways using Gene 
      Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway 
      analyses. RESULTS: The expression levels of 13 miRNAs were remarkably upregulated 
      while the other 35 miRNAs were significantly downregulated, in women with IHCP 
      (P</=0.05) when compared with healthy pregnant women. The areas under the curves of 
      miRNA-7706, miRNA-877-3p, and miRNA-128-3p were higher than 0.90, indicating more 
      reliable diagnosis of IHCP. The Pearson analysis showed that the levels of these 
      miRNAs were positively correlated to TBA level. Additionally, the results of 
      bioinformatics analysis revealed that the differentially expressed miRNAs mainly 
      influenced fatty acid biosynthesis, the endoplasmic reticulum ubiquitin ligase 
      complex, and the p53, and mammalian target of rapamycin (mTOR) and AMP-activated 
      protein kinase (AMPK) signaling pathways. CONCLUSION: The panel of three-miRNAs 
      (miRNA-7706, miRNA-877-3p, and miRNA-128-3p) may be a useful noninvasive 
      diagnostic biomarker of IHCP.
CI  - AJTR Copyright (c) 2022.
FAU - Zu, Yue
AU  - Zu Y
AD  - Tongji Hospital, Tongji Medical College, Huazhong University of Science and 
      Technology Wuhan 430030, Hubei, China.
FAU - Guo, Sheng
AU  - Guo S
AD  - The First Affiliated Hospital of Xinxiang Medical University Weihui, Henan, 
      China.
FAU - Li, Guodong
AU  - Li G
AD  - Tongji Hospital, Tongji Medical College, Huazhong University of Science and 
      Technology Wuhan 430030, Hubei, China.
FAU - Gao, Qianyan
AU  - Gao Q
AD  - Tongji Hospital, Tongji Medical College, Huazhong University of Science and 
      Technology Wuhan 430030, Hubei, China.
FAU - Wang, Ximin
AU  - Wang X
AD  - Tongji Hospital, Tongji Medical College, Huazhong University of Science and 
      Technology Wuhan 430030, Hubei, China.
FAU - Zhang, Chengliang
AU  - Zhang C
AD  - Tongji Hospital, Tongji Medical College, Huazhong University of Science and 
      Technology Wuhan 430030, Hubei, China.
FAU - Liu, Dong
AU  - Liu D
AD  - Tongji Hospital, Tongji Medical College, Huazhong University of Science and 
      Technology Wuhan 430030, Hubei, China.
LA  - eng
PT  - Journal Article
DEP - 20220915
PL  - United States
TA  - Am J Transl Res
JT  - American journal of translational research
JID - 101493030
PMC - PMC9556493
OTO - NOTNLM
OT  - bioinformatics analysis
OT  - diagnostic biomarkers
OT  - intrahepatic cholestasis of pregnancy
OT  - miRNAs
OT  - microarray analysis
COIS- None.
EDAT- 2022/10/18 06:00
MHDA- 2022/10/18 06:01
PMCR- 2022/09/15
CRDT- 2022/10/17 04:46
PHST- 2022/06/06 00:00 [received]
PHST- 2022/08/08 00:00 [accepted]
PHST- 2022/10/17 04:46 [entrez]
PHST- 2022/10/18 06:00 [pubmed]
PHST- 2022/10/18 06:01 [medline]
PHST- 2022/09/15 00:00 [pmc-release]
PST - epublish
SO  - Am J Transl Res. 2022 Sep 15;14(9):6763-6773. eCollection 2022.