PMID- 36247296
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221019
IS  - 1943-8141 (Print)
IS  - 1943-8141 (Electronic)
IS  - 1943-8141 (Linking)
VI  - 14
IP  - 9
DP  - 2022
TI  - Relationship between microRNA expression and inflammatory factors in patients 
      with both type 2 diabetes mellitus and periodontal disease.
PG  - 6627-6637
AB  - OBJECTIVE: To investigate the expression of single-stranded microRNAs (miRNAs) in 
      serum and gingival crevicular fluid of patients with type 2 diabetes mellitus 
      (T2DM) complicated by periodontal disease and its correlation with inflammatory 
      factors. METHODS: Twenty-six periodontitis patients without diabetes mellitus 
      (periodontal group), 24 patients with T2DM (T2DM group), 22 patients with both 
      T2DM and periodontal disease (comorbid group), and 25 healthy individuals without 
      a history of periodontal disease (healthy group) were recruited respectively. 
      Serum and gingival crevicular fluid specimens were collected to detect the 
      expression levels of miRNAs and inflammatory factors including serum tumor 
      necrosis factor-alpha (TNF-alpha), interleukin-10 (IL-10), and transforming growth 
      factor-beta (TGF-beta), and their correlations were also investigated. RESULTS: Eleven 
      miRNAs were detected in the gingival crevicular tissue of all subjects. The 
      expression of miR-223 and miR-200b in serum and gingival crevicular fluid was 
      elevated higher in the comorbid group than in the other three groups (P<0.05), 
      and their expressions in gingival crevicular fluid contributed to the 
      differential diagnosis of periodontal disease from diabetes mellitus comorbid 
      with periodontal disease (P<0.05). Gingival crevicular fluid miR-223 expression 
      was positively associated with TNF-alpha, clinical attachment loss (CAL), and probing 
      pocket depth (PPD) in the periodontal group, while negatively associated with 
      IL-10 (P<0.05), and so was gingival crevicular fluid miR-200b expression with 
      TNF-alpha, CAL, and PPD (P<0.05). In the comorbid group, gingival crevicular fluid 
      miR-223 expression showed a positive correlation with TNF-alpha, CAL, and PPD 
      (P<0.05), and so did gingival crevicular fluid miR-200b expression with TNF-alpha, 
      CAL, and PPD (P<0.05). CONCLUSIONS: Our findings indicate a close link between 
      the levels of miR-223 and miR-200b in serum and gingival crevicular fluid and 
      susceptibility to T2DM as well as the pathogenesis of periodontal disease.
CI  - AJTR Copyright (c) 2022.
FAU - Liu, Lu
AU  - Liu L
AD  - Department of Stomatology, Cangzhou Central Hospital Cangzhou, Hebei, China.
FAU - Xiao, Zunsheng
AU  - Xiao Z
AD  - Department of Stomatology, Cangzhou Central Hospital Cangzhou, Hebei, China.
FAU - Ding, Wencui
AU  - Ding W
AD  - The First Department of Endocrine Diabetes, Cangzhou Central Hospital Cangzhou, 
      Hebei, China.
FAU - Wen, Chao
AU  - Wen C
AD  - Department of Clinical Laboratoy, Cangzhou Central Hospital Cangzhou, Hebei, 
      China.
FAU - Ge, Chao
AU  - Ge C
AD  - Department of Stomatology, Cangzhou Central Hospital Cangzhou, Hebei, China.
FAU - Xu, Ke
AU  - Xu K
AD  - Department of Stomatology, Cangzhou Central Hospital Cangzhou, Hebei, China.
FAU - Cao, Sumin
AU  - Cao S
AD  - Department of Stomatology, Cangzhou Central Hospital Cangzhou, Hebei, China.
LA  - eng
PT  - Journal Article
DEP - 20220915
PL  - United States
TA  - Am J Transl Res
JT  - American journal of translational research
JID - 101493030
PMC - PMC9556472
OTO - NOTNLM
OT  - T2DM
OT  - gingival crevicular fluid
OT  - periodontal disease
OT  - single-stranded microRNA
COIS- None.
EDAT- 2022/10/18 06:00
MHDA- 2022/10/18 06:01
PMCR- 2022/09/15
CRDT- 2022/10/17 04:46
PHST- 2021/05/19 00:00 [received]
PHST- 2021/07/31 00:00 [accepted]
PHST- 2022/10/17 04:46 [entrez]
PHST- 2022/10/18 06:00 [pubmed]
PHST- 2022/10/18 06:01 [medline]
PHST- 2022/09/15 00:00 [pmc-release]
PST - epublish
SO  - Am J Transl Res. 2022 Sep 15;14(9):6627-6637. eCollection 2022.