PMID- 36249459
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221019
IS  - 2228-7930 (Print)
IS  - 2228-7949 (Electronic)
IS  - 2228-7930 (Linking)
VI  - 12
IP  - 5
DP  - 2022 Sep-Oct
TI  - Effects of some anti-diabetic herbal extracts on the insulin-degrading enzyme in 
      human colon cancer Caco-2 cell line.
PG  - 548-558
LID - 10.22038/AJP.2022.19982 [doi]
AB  - OBJECTIVE: Type 2 diabetes mellitus (T2DM) is a condition characterized by 
      insufficient insulin production or insulin resistance. The insulin-degrading 
      enzyme (IDE) is responsible for degrading insulin and is a potential drug target 
      for T2DM treatment. Numerous activities have been proposed for plant extracts, 
      but research on the effects of plant extracts on IDE expression and activity is 
      riddled with drawbacks. MATERIALS AND METHODS: We investigated the effect of 
      Phaseolus vulgaris, Allium cepa, Portulaca oleracea, Cinnamomum verum, and 
      Citrullus colocynthis extracts on the expression and activity of IDE in the 
      Caco-2 cell line. RESULTS: Findings of RT-PCR showed that IDE gene expression was 
      reduced following treatment with P. vulgaris, C. colocynthis, and C. verum 
      extracts. The results of IDE activity with fluorogenic peptide substrate V also 
      indicated that P. vulgaris, C. colocynthis, and P. oleracea extracts reduced IDE 
      activity in a significant and dose-dependent manner. CONCLUSION: The 
      hydroalcoholic extracts studied, except for A. cepa, can prevent insulin 
      degradation by reducing the expression and activity of the IDE enzyme. This new 
      insight into the effects of herbal medicines on IDE activity can help future 
      studies.
FAU - Norouzi, Mahtab
AU  - Norouzi M
AD  - Department of Clinical Biochemistry, Faculty of Medicine, Mashhad University of 
      Medical Sciences, Mashhad, Iran.
AD  - Student Research Committee, Faculty of Medicine, Mashhad University of Medical 
      Sciences, Mashhad, Iran.
FAU - Saghi, Hossein
AU  - Saghi H
AD  - Student Research Committee, Faculty of Medicine, Mashhad University of Medical 
      Sciences, Mashhad, Iran.
FAU - Mohebbati, Reza
AU  - Mohebbati R
AD  - Department of Physiology, Faculty of Medicine, Gonabad University of Medical 
      Sciences, Gonabad, Iran.
AD  - Applied Biomedical Research Center, Mashhad University of Medical Sciences, 
      Mashhad, Iran.
FAU - Mirzavi, Farshad
AU  - Mirzavi F
AD  - Department of Clinical Biochemistry, Faculty of Medicine, Mashhad University of 
      Medical Sciences, Mashhad, Iran.
FAU - Afshari, Amir Reza
AU  - Afshari AR
AD  - Department of Physiology and Pharmacology, Faculty of Medicine, North Khorasan 
      University of Medical Sciences, Bojnurd, Iran.
FAU - Soukhtanloo, Mohammad
AU  - Soukhtanloo M
AD  - Department of Clinical Biochemistry, Faculty of Medicine, Mashhad University of 
      Medical Sciences, Mashhad, Iran.
AD  - Pharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad 
      University of Medical Sciences, Mashhad, Iran.
LA  - eng
PT  - Journal Article
PL  - Iran
TA  - Avicenna J Phytomed
JT  - Avicenna journal of phytomedicine
JID - 101586220
PMC - PMC9516401
OTO - NOTNLM
OT  - Allium cepa
OT  - Cinnamomum verum
OT  - Citrullus colocynthis
OT  - Insulin-degrading enzyme Phaseolus vulgaris
OT  - Portulaca oleracea
COIS- The authors have declared that there is no conflict of interest.
EDAT- 2022/10/18 06:00
MHDA- 2022/10/18 06:01
PMCR- 2022/09/01
CRDT- 2022/10/17 05:23
PHST- 2021/11/08 00:00 [received]
PHST- 2022/01/24 00:00 [revised]
PHST- 2022/02/14 00:00 [accepted]
PHST- 2022/10/17 05:23 [entrez]
PHST- 2022/10/18 06:00 [pubmed]
PHST- 2022/10/18 06:01 [medline]
PHST- 2022/09/01 00:00 [pmc-release]
AID - 10.22038/AJP.2022.19982 [doi]
PST - ppublish
SO  - Avicenna J Phytomed. 2022 Sep-Oct;12(5):548-558. doi: 10.22038/AJP.2022.19982.