PMID- 36250225 OWN - NLM STAT- MEDLINE DCOM- 20221130 LR - 20221130 IS - 1098-2825 (Electronic) IS - 0887-8013 (Print) IS - 0887-8013 (Linking) VI - 36 IP - 11 DP - 2022 Nov TI - Detection of interstitial pneumonia with autoimmune features and idiopathic pulmonary fibrosis are enhanced by involvement of matrix metalloproteinases levels and clinical diagnosis. PG - e24734 LID - 10.1002/jcla.24734 [doi] LID - e24734 AB - BACKGROUND: Higher detection of interstitial pneumonia with autoimmune features (IPAF), and idiopathic pulmonary fibrosis (IPF), has significant diagnostic and therapeutic implications. Some matrix metalloproteinases (MMPs) have become reliable diagnostic biomarkers in IPAF and IPF in previous studies, yet relevant reliability remains to be recognized. MATERIALS AND METHODS: In this study, 36 ILDs patients, including 31 IPAF patients (Mean +/- SD, 50.20 +/- 5.10 years; 16 [51.6%] females) and five IPF patients (Mean +/- SD, 61.20 +/- 6.73 years; one [20.0%] females) were retrospectively enrolled. Serial serum samples were collected from patients with IPAF and IPF between January 2019 and December 2020. Notably, Serum MMPs levels were measured by U-PLEX Biomarker Group 1(Human) Multiplex Assays (MSD, USA). RESULTS: A combination of MMPs and combinatorial biomarkers was strongly associated with clinical subjects in this study (AUC, 0.597 for Stability vs. Improvement and 0.756 for Stability vs. Exacerbation). Importantly, the AUC of MMP-12 reaches 0.730 (p < 0.05, Stability AUC vs. Improvement AUC) while MMP-13 reaches 0.741 (p < 0.05, Stability AUC vs. Exacerbation AUC) showed better performance than other MMPs in two comparisons. CONCLUSIONS: Clinical risk factors and MMPs are strongly associated with either stratification of the disease of progression of IPAF or in two IPAF and IPF independent cohorts. To our knowledge, this is the first to illustrate that MMP-12 and MMP-13 may be expected to become typical promising biomarkers in Improvement - IPAF and Exacerbation - IPAF, respectively. CI - (c) 2022 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC. FAU - Liu, Mingtao AU - Liu M AUID- ORCID: 0000-0002-8951-8361 AD - Department of Allergy and Clinical Immunology, Guangzhou Institute of Respiratory Health, State Key Laboratory of Respiratory Disease, National Clinical Research Center of Respiratory Disease, First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China. FAU - Xue, Mingshan AU - Xue M AD - Department of Allergy and Clinical Immunology, Guangzhou Institute of Respiratory Health, State Key Laboratory of Respiratory Disease, National Clinical Research Center of Respiratory Disease, First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China. FAU - Zhang, Teng AU - Zhang T AD - Faculty of Health Sciences, University of Macau, Taipa, China. FAU - Lin, Runpei AU - Lin R AD - Department of Allergy and Clinical Immunology, Guangzhou Institute of Respiratory Health, State Key Laboratory of Respiratory Disease, National Clinical Research Center of Respiratory Disease, First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China. FAU - Guo, Baojun AU - Guo B AD - Department of Allergy and Clinical Immunology, Guangzhou Institute of Respiratory Health, State Key Laboratory of Respiratory Disease, National Clinical Research Center of Respiratory Disease, First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China. FAU - Chen, Youpeng AU - Chen Y AUID- ORCID: 0000-0002-9677-2333 AD - Guangzhou Medical University, Guangzhou, China. FAU - Cheng, Zhangkai J AU - Cheng ZJ AUID- ORCID: 0000-0002-8621-4119 AD - Department of Allergy and Clinical Immunology, Guangzhou Institute of Respiratory Health, State Key Laboratory of Respiratory Disease, National Clinical Research Center of Respiratory Disease, First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China. FAU - Sun, Baoqing AU - Sun B AUID- ORCID: 0000-0002-1671-0723 AD - Department of Allergy and Clinical Immunology, Guangzhou Institute of Respiratory Health, State Key Laboratory of Respiratory Disease, National Clinical Research Center of Respiratory Disease, First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China. LA - eng GR - 81960023/National Natural Science Foundation of China/ GR - 81871736/National Natural Science Foundation of China/ GR - 20192048/Bureau of Traditional Chinese Medicine Scientific Research Project of Guangdong/ GR - 201804020043/Science and Technology Innovation Committee Project of Guangzhou/ GR - 201831802/Key projects of Guangzhou Education Bureau/ GR - SKLRD-OP-201809/Open Project of State Key Laboratory of Respiratory Disease/ GR - SKLRD-OP-201803/Open Project of State Key Laboratory of Respiratory Disease/ PT - Journal Article DEP - 20221017 PL - United States TA - J Clin Lab Anal JT - Journal of clinical laboratory analysis JID - 8801384 RN - 0 (Biomarkers) RN - EC 3.4.24.65 (Matrix Metalloproteinase 12) RN - EC 3.4.24.- (Matrix Metalloproteinase 13) SB - IM MH - Female MH - Humans MH - Male MH - Biomarkers MH - *Idiopathic Pulmonary Fibrosis/diagnosis/complications MH - *Lung Diseases, Interstitial/diagnosis/complications MH - Matrix Metalloproteinase 12 MH - Matrix Metalloproteinase 13 MH - Reproducibility of Results MH - Retrospective Studies MH - Middle Aged MH - Aged PMC - PMC9701863 OTO - NOTNLM OT - clinical diagnosis OT - detection OT - idiopathic pulmonary fibrosis OT - interstitial pneumonia with autoimmune features OT - matrix metalloproteinases COIS- The authors declare no conflict of interest. EDAT- 2022/10/18 06:00 MHDA- 2022/11/30 06:00 PMCR- 2022/10/17 CRDT- 2022/10/17 05:37 PHST- 2022/09/06 00:00 [revised] PHST- 2022/07/21 00:00 [received] PHST- 2022/10/05 00:00 [accepted] PHST- 2022/10/18 06:00 [pubmed] PHST- 2022/11/30 06:00 [medline] PHST- 2022/10/17 05:37 [entrez] PHST- 2022/10/17 00:00 [pmc-release] AID - JCLA24734 [pii] AID - 10.1002/jcla.24734 [doi] PST - ppublish SO - J Clin Lab Anal. 2022 Nov;36(11):e24734. doi: 10.1002/jcla.24734. Epub 2022 Oct 17.