PMID- 36250430
OWN - NLM
STAT- MEDLINE
DCOM- 20221101
LR  - 20221225
IS  - 1473-558X (Electronic)
IS  - 0959-4965 (Linking)
VI  - 33
IP  - 17
DP  - 2022 Dec 14
TI  - Transplantation of olfactory ensheathing cells decreases local and serological 
      monocyte chemoattractant protein 1 level during the acute phase of rat spinal 
      cord injury.
PG  - 729-741
LID - 10.1097/WNR.0000000000001839 [doi]
AB  - OBJECTIVES: Monocyte chemoattractant protein 1 (MCP1) is one of the most 
      upregulated cytokines in the spinal cord and serum throughout acute spinal cord 
      injury (SCI). Olfactory ensheathing cells (OECs) transplantation improves SCI 
      through multiple mechanisms, including immunomodulation. Our study aimed to 
      investigate whether OECs ameliorate acute inflammation after SCI by modulating 
      MCP1 expression. METHODS: We established a standardized clinically relevant 
      contusion model using the NYU impactor. OECs were administered to the injured 
      spinal cord via microinjection 30 minutes after injury. Rat locomotor functions 
      were assessed by the Basso-Beattie-Bresnahan scale score. Time-course 
      histopathological (H&E and IHC) analyses were performed to record rapid changes 
      in acute inflammation at lesion epicenters. Serum MCP1 level was detected by 
      ELISA assay. RESULTS: BBB scores showed improved locomotor functional recoveries 
      in the OECs transplantation group after SCI ( P < 0.05). Staining of H&E and CD68 
      illustrated that OECs transplantation attenuated inflammatory response by 
      reducing lesion areas and infiltrating myeloid cell numbers. We further revealed 
      significantly decreased MCP1 levels in the spinal cord and serum after OECs 
      transplantation ( P < 0.05). Noteworthily, distinct expression levels of MCP1 
      were found in rats undergoing a mild injury (cord impacted from a 10-mm height) 
      compared to the moderate injury (25-mm) group. CONCLUSION: Our study reports that 
      transplantation of OECs promotes locomotor functional recovery after SCI and 
      alleviates acute inflammation by decreasing local and serological MCP1 levels. We 
      provide preliminary evidence that MCP1 might serve as a potential biomarker to 
      reflect the severity of SCI, which is of great interest in future studies.
CI  - Copyright (c) 2022 Wolters Kluwer Health, Inc. All rights reserved.
FAU - Wang, Yiqun
AU  - Wang Y
AD  - Department of Orthopedics.
FAU - Gong, Minjie
AU  - Gong M
AD  - Department of Otolaryngology, Head and Neck Surgery, Second Affiliated Hospital, 
      Xi'an Jiaotong University.
FAU - He, Xijing
AU  - He X
AD  - Department of Orthopedics.
AD  - Department of Orthopedics, Xi'an International Medical Center Hospital.
FAU - Wu, Yutong
AU  - Wu Y
AD  - Department of Otolaryngology, Head and Neck Surgery, Honghui Hospital, Xi'an 
      Jiaotong University, Xi'an, Shaanxi, China.
FAU - Li, Yuhuan
AU  - Li Y
AD  - Department of Orthopedics.
FAU - Li, Jialiang
AU  - Li J
AD  - Department of Orthopedics.
FAU - Wen, Zhijing
AU  - Wen Z
AD  - Department of Orthopedics.
FAU - Wang, Yibin
AU  - Wang Y
AD  - Department of Orthopedics.
FAU - Gu, Pengzhen
AU  - Gu P
AD  - Department of Orthopedics.
FAU - Li, Haopeng
AU  - Li H
AD  - Department of Orthopedics.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20221017
PL  - England
TA  - Neuroreport
JT  - Neuroreport
JID - 9100935
RN  - 0 (Chemokine CCL2)
SB  - IM
MH  - Rats
MH  - Animals
MH  - *Chemokine CCL2/metabolism
MH  - Rats, Sprague-Dawley
MH  - Olfactory Bulb/metabolism
MH  - *Spinal Cord Injuries/metabolism
MH  - Recovery of Function/physiology
MH  - Spinal Cord/metabolism
MH  - Cell Transplantation
MH  - Inflammation/metabolism
EDAT- 2022/10/18 06:00
MHDA- 2022/11/02 06:00
CRDT- 2022/10/17 05:52
PHST- 2022/10/18 06:00 [pubmed]
PHST- 2022/11/02 06:00 [medline]
PHST- 2022/10/17 05:52 [entrez]
AID - 00001756-202212010-00003 [pii]
AID - 10.1097/WNR.0000000000001839 [doi]
PST - ppublish
SO  - Neuroreport. 2022 Dec 14;33(17):729-741. doi: 10.1097/WNR.0000000000001839. Epub 
      2022 Oct 17.