PMID- 36251233 OWN - NLM STAT- MEDLINE DCOM- 20221229 LR - 20221229 IS - 1559-1182 (Electronic) IS - 0893-7648 (Linking) VI - 60 IP - 1 DP - 2023 Jan TI - The Pivotal Role of Neuropeptide Crosstalk from Ventromedial-PACAP to Dorsomedial-Galanin in the Appetite Regulation in the Mouse Hypothalamus. PG - 171-182 LID - 10.1007/s12035-022-03084-y [doi] AB - We have previously shown that pituitary adenylate cyclase-activating polypeptide (PACAP) in the ventromedial hypothalamus (VMH) enhances feeding during the dark cycle and after fasting, and inhibits feeding during the light cycle. On the other hand, galanin is highly expressed in the hypothalamus and has been reported to be involved in feeding regulation. In this study, we investigated the involvement of the VMH-PACAP to the dorsomedial hypothalamus (DMH)-galanin signaling in the regulation of feeding. Galanin expression in the hypothalamus was significantly increased with fasting, but this increment was canceled in PACAP-knockout (KO) mice. Furthermore, overexpression of PACAP in the VMH increased the expression of galanin, while knockdown (KD) of PACAP in the VMH decreased the expression of galanin, indicating that the expression of galanin in the hypothalamus might be regulated by PACAP in the VMH. Therefore, we expressed the synaptophysin-EGFP fusion protein (SypEGFP) in PACAP neurons in the VMH and visualized the neural projection to the hypothalamic region where galanin was highly expressed. A strong synaptophysin-EGFP signal was observed in the DMH, indicating that PACAP-expressing cells of the VMH projected to the DMH. Furthermore, galanin immunostaining in the DMH showed that galanin expression was weak in PACAP-KO mice. When galanin in the DMH was knocked down, food intake during the dark cycle and after fasting was decreased, and food intake during the light cycle was increased, as in PACAP-KO mice. These results indicated that galanin in the DMH may regulate the feeding downstream of PACAP in the VMH. CI - (c) 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. FAU - Kambe, Yuki AU - Kambe Y AUID- ORCID: 0000-0003-1484-0628 AD - Department of Pharmacology, Graduate School of Medical and Dental Science, Kagoshima University, Sakuragaoka 8-35-1, Kagoshima-shi, Kagoshima, 890-8544, Japan. ukikambe@m3.kufm.kagoshima-u.ac.jp. FAU - Nguyen, Thanh Trung AU - Nguyen TT AUID- ORCID: 0000-0002-6880-8757 AD - Department of Pharmacology, Graduate School of Medical and Dental Science, Kagoshima University, Sakuragaoka 8-35-1, Kagoshima-shi, Kagoshima, 890-8544, Japan. FAU - Yasaka, Toshiharu AU - Yasaka T AUID- ORCID: 0000-0002-8589-3682 AD - Department of Health and Nutrition, Faculty of Health Sciences, University of Health and Welfare, Shimamicho 1398, Kita-ku, Niigata, 950-3198, Japan. FAU - Nguyen, Thu Thi AU - Nguyen TT AD - Department of Pharmacology, Graduate School of Medical and Dental Science, Kagoshima University, Sakuragaoka 8-35-1, Kagoshima-shi, Kagoshima, 890-8544, Japan. FAU - Sameshima, Yoshimune AU - Sameshima Y AD - Department of Pharmacology, Graduate School of Medical and Dental Science, Kagoshima University, Sakuragaoka 8-35-1, Kagoshima-shi, Kagoshima, 890-8544, Japan. FAU - Hashiguchi, Kohei AU - Hashiguchi K AD - Department of Pharmacology, Graduate School of Medical and Dental Science, Kagoshima University, Sakuragaoka 8-35-1, Kagoshima-shi, Kagoshima, 890-8544, Japan. FAU - Shintani, Norihito AU - Shintani N AUID- ORCID: 0000-0002-7850-9064 AD - Laboratory of Pharmacology, School of Pharmaceutical Sciences, Wakayama Medical University, 25-1 Shichibancho, Wakayama-shi, Wakayama, 640-8156, Japan. AD - Laboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka, 565-0871, Japan. FAU - Hashimoto, Hitoshi AU - Hashimoto H AUID- ORCID: 0000-0001-6548-4016 AD - Laboratory of Pharmacology, School of Pharmaceutical Sciences, Wakayama Medical University, 25-1 Shichibancho, Wakayama-shi, Wakayama, 640-8156, Japan. AD - United Graduate School of Child Development, Osaka University, Kanazawa University and Hamamatsu University School of Medicine, Chiba University and University of Fukui, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan. AD - Division of Bioscience, Institute for Datability Science, Osaka University, 2-8 Yamadaoka, Suita, Osaka, 565-0871, Japan. AD - Transdimensional Life Imaging Division, Institute for Open and Transdisciplinary Research Initiatives, Osaka University, 1-1 Yamadaoka, Suita, Osaka, 565-0871, Japan. AD - Department of Molecular Pharmaceutical Sciences, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan. FAU - Kurihara, Takashi AU - Kurihara T AUID- ORCID: 0000-0003-4001-6771 AD - Department of Pharmacology, Graduate School of Medical and Dental Science, Kagoshima University, Sakuragaoka 8-35-1, Kagoshima-shi, Kagoshima, 890-8544, Japan. FAU - Miyata, Atsuro AU - Miyata A AUID- ORCID: 0000-0003-3959-331X AD - Department of Pharmacology, Graduate School of Medical and Dental Science, Kagoshima University, Sakuragaoka 8-35-1, Kagoshima-shi, Kagoshima, 890-8544, Japan. LA - eng GR - JPJP20K07067/Japan Society for the Promotion of Science/ GR - JP20H03429/Japan Society for the Promotion of Science/ GR - JP18K07394/Japan Society for the Promotion of Science/ GR - JP21K19335/Japan Society for the Promotion of Science/ GR - JP20H00492/Japan Society for the Promotion of Science/ GR - JP19K07121/Japan Society for the Promotion of Science/ GR - JP18H05416/The Ministry of Education,Culture,Sports,Science and Technology/ GR - JP21dm0207117/Japan Agency for Medical Research and Development/ PT - Journal Article DEP - 20221017 PL - United States TA - Mol Neurobiol JT - Molecular neurobiology JID - 8900963 RN - 88813-36-9 (Galanin) RN - 0 (Pituitary Adenylate Cyclase-Activating Polypeptide) RN - 0 (Synaptophysin) SB - IM MH - Animals MH - Mice MH - Appetite Regulation MH - Galanin/metabolism MH - *Hypothalamus/metabolism MH - *Pituitary Adenylate Cyclase-Activating Polypeptide/metabolism MH - Synaptophysin/metabolism OTO - NOTNLM OT - Appetite OT - DMH OT - Galanin OT - Hypothalamus OT - PACAP OT - VMH EDAT- 2022/10/18 06:00 MHDA- 2022/12/21 06:00 CRDT- 2022/10/17 11:25 PHST- 2022/04/06 00:00 [received] PHST- 2022/10/03 00:00 [accepted] PHST- 2022/10/18 06:00 [pubmed] PHST- 2022/12/21 06:00 [medline] PHST- 2022/10/17 11:25 [entrez] AID - 10.1007/s12035-022-03084-y [pii] AID - 10.1007/s12035-022-03084-y [doi] PST - ppublish SO - Mol Neurobiol. 2023 Jan;60(1):171-182. doi: 10.1007/s12035-022-03084-y. Epub 2022 Oct 17.