PMID- 36251856
OWN - NLM
STAT- MEDLINE
DCOM- 20221230
LR  - 20230524
IS  - 1529-4242 (Electronic)
IS  - 0032-1052 (Print)
IS  - 0032-1052 (Linking)
VI  - 151
IP  - 1
DP  - 2023 Jan 1
TI  - Is There a Wound Recontamination by Eluates with High Bacterial Load in 
      Negative-Pressure Wound Therapy with Instillation and Dwell Time?
PG  - 136e-147e
LID - 10.1097/PRS.0000000000009770 [doi]
AB  - BACKGROUND: This study investigated bacterial colonization of the foam eluate 
      after negative-pressure wound therapy (NPWT) with instillation and dwell time 
      (NPWTi-d) to obtain an indication of possible recontamination of the wound during 
      NPWTi-d. To detect bacterial colonization and the extent of planktonic and 
      nonplanktonic bioburden as comprehensively as possible, routine culture and 
      molecular biology methods were used. METHODS: Before (time point 1) and after 
      (median 3.0 days; time point 2) NPWT ( n = 15) and NPWTi-d with antiseptic 
      installation ( n = 15), wound bed [22 acute, eight chronic wounds; median age, 51 
      years (range, 24 to 91); 26 men], foam, and eluate were examined by routine 
      culture methods and fluorescence in situ hybridization (FISH), polymerase chain 
      reaction, and FISH sequencing (FISHseq). RESULTS: At time point 2, 94.9% (37 of 
      39) of the pathogens identifiable in the eluate were also detected in the wound 
      bed. Foam and eluate were always bacterially contaminated. NPWTi-d resulted in a 
      significant reduction in the number of pathogen species compared with NPWT 
      (NPWTi-d, time point 1 versus time point 2: P = 0.026; NPWT, time point 1 versus 
      time point 2: not significant). Routine culture of wound bed samples at time 
      point 2 identified only 28 of 52 (53.8%) of the pathogens, whereas examination of 
      wound bed, foam, and eluate and additional FISHseq use detected 50 of 52 (96.2%) 
      of the bacterial species. FISHseq identified biofilm in one and microcolonies in 
      10 wounds (time point 2). CONCLUSIONS: The bacterial load of the foam is flushed 
      back into the wound during NPWTi-d. FISHseq should be used in addition to the 
      routine culture method when pathogen identification and detection of 
      nonplanktonic bacterial growth is particularly important for the patient's 
      therapy. CLINICAL QUESTION/LEVEL OF EVIDENCE: Therapeutic, V.
CI  - Copyright (c) 2022 The Authors. Published by Wolters Kluwer Health, Inc. on behalf 
      of the American Society of Plastic Surgeons. All rights reserved.
FAU - Stichling, Marcus
AU  - Stichling M
AD  - From Trauma and Orthopedic Surgery, Septic and Reconstructive Surgery, Research 
      and Treatment Center Septic Defect Woundsand.
FAU - Wiessner, Alexandra
AU  - Wiessner A
AD  - Biofilmcenter, Institute for Microbiology, Infectious Diseases, and Immunology, 
      Charite-University Medicine Berlin and MoKi Analytics GmbH.
FAU - Kikhney, Judith
AU  - Kikhney J
AD  - Biofilmcenter, Institute for Microbiology, Infectious Diseases, and Immunology, 
      Charite-University Medicine Berlin and MoKi Analytics GmbH.
FAU - Gatzer, Renate
AU  - Gatzer R
AD  - Department of Microbiology, Federal Armed Forces of Germany, Bundeswehr 
      (Military) Academic Hospital.
FAU - Muller, Martin
AU  - Muller M
AD  - Department of Microbiology, Federal Armed Forces of Germany, Bundeswehr 
      (Military) Academic Hospital.
FAU - Scheuermann-Poley, Catharina
AU  - Scheuermann-Poley C
AD  - From Trauma and Orthopedic Surgery, Septic and Reconstructive Surgery, Research 
      and Treatment Center Septic Defect Woundsand.
FAU - Moter, Annette
AU  - Moter A
AD  - Biofilmcenter, Institute for Microbiology, Infectious Diseases, and Immunology, 
      Charite-University Medicine Berlin and MoKi Analytics GmbH.
FAU - Willy, Christian
AU  - Willy C
AD  - From Trauma and Orthopedic Surgery, Septic and Reconstructive Surgery, Research 
      and Treatment Center Septic Defect Woundsand.
LA  - eng
PT  - Journal Article
DEP - 20221017
PL  - United States
TA  - Plast Reconstr Surg
JT  - Plastic and reconstructive surgery
JID - 1306050
SB  - IM
MH  - *Wound Healing
MH  - *Negative-Pressure Wound Therapy/methods
MH  - Bacterial Load
MH  - In Situ Hybridization, Fluorescence
MH  - Therapeutic Irrigation/methods
PMC - PMC9788932
EDAT- 2022/10/18 06:00
MHDA- 2022/12/31 06:00
PMCR- 2022/12/23
CRDT- 2022/10/17 14:23
PHST- 2022/10/18 06:00 [pubmed]
PHST- 2022/12/31 06:00 [medline]
PHST- 2022/10/17 14:23 [entrez]
PHST- 2022/12/23 00:00 [pmc-release]
AID - 00006534-202301000-00036 [pii]
AID - 10.1097/PRS.0000000000009770 [doi]
PST - ppublish
SO  - Plast Reconstr Surg. 2023 Jan 1;151(1):136e-147e. doi: 
      10.1097/PRS.0000000000009770. Epub 2022 Oct 17.