PMID- 36252028
OWN - NLM
STAT- MEDLINE
DCOM- 20221019
LR  - 20230803
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 119
IP  - 43
DP  - 2022 Oct 25
TI  - Arabidopsis ORP2A mediates ER-autophagosomal membrane contact sites and regulates 
      PI3P in plant autophagy.
PG  - e2205314119
LID - 10.1073/pnas.2205314119 [doi]
LID - e2205314119
AB  - Autophagy is an intracellular degradation system for cytoplasmic constituents 
      which is mediated by the formation of a double-membrane organelle termed the 
      autophagosome and its subsequent fusion with the lysosome/vacuole. The formation 
      of the autophagosome requires membrane from the endoplasmic reticulum (ER) and is 
      tightly regulated by a series of autophagy-related (ATG) proteins and lipids. 
      However, how the ER contacts autophagosomes and regulates autophagy remain 
      elusive in plants. In this study, we identified and demonstrated the roles of 
      Arabidopsis oxysterol-binding protein-related protein 2A (ORP2A) in mediating 
      ER-autophagosomal membrane contacts and autophagosome biogenesis. We showed that 
      ORP2A localizes to both ER-plasma membrane contact sites (EPCSs) and 
      autophagosomes, and that ORP2A interacts with both the ER-localized 
      VAMP-associated protein (VAP) 27-1 and ATG8e on the autophagosomes to mediate the 
      membrane contact sites (MCSs). In ORP2A artificial microRNA knockdown (KD) 
      plants, seedlings display retarded growth and impaired autophagy levels. Both 
      ATG1a and ATG8e accumulated and associated with the ER membrane in ORP2A KD 
      lines. Moreover, ORP2A binds multiple phospholipids and shows colocalization with 
      phosphatidylinositol 3-phosphate (PI3P) in vivo. Taken together, ORP2A mediates 
      ER-autophagosomal MCSs and regulates autophagy through PI3P redistribution.
FAU - Ye, Hao
AU  - Ye H
AUID- ORCID: 0000-0002-0862-1843
AD  - School of Life Sciences, Centre for Cell & Developmental Biology, State Key 
      Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Hong Kong, 
      China.
FAU - Gao, Jiayang
AU  - Gao J
AD  - School of Life Sciences, Centre for Cell & Developmental Biology, State Key 
      Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Hong Kong, 
      China.
FAU - Liang, Zizhen
AU  - Liang Z
AUID- ORCID: 0000-0002-8622-0674
AD  - School of Life Sciences, Centre for Cell & Developmental Biology, State Key 
      Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Hong Kong, 
      China.
FAU - Lin, Youshun
AU  - Lin Y
AD  - School of Life Sciences, Centre for Cell & Developmental Biology, State Key 
      Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Hong Kong, 
      China.
FAU - Yu, Qianyi
AU  - Yu Q
AD  - School of Life Sciences, Centre for Cell & Developmental Biology, State Key 
      Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Hong Kong, 
      China.
FAU - Huang, Shuxian
AU  - Huang S
AD  - School of Life Sciences, Centre for Cell & Developmental Biology, State Key 
      Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Hong Kong, 
      China.
FAU - Jiang, Liwen
AU  - Jiang L
AD  - School of Life Sciences, Centre for Cell & Developmental Biology, State Key 
      Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Hong Kong, 
      China.
AD  - The Chinese University of Hong Kong Shenzhen Research Institute, Shenzhen, 
      518057, China.
AD  - Institute of Plant Molecular Biology and Agricultural Biotechnology, The Chinese 
      University of Hong Kong, Hong Kong, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20221017
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Autophagy-Related Proteins)
RN  - 0 (MicroRNAs)
RN  - 0 (Oxysterols)
SB  - IM
CIN - Autophagy. 2023 Sep;19(9):2616-2617. PMID: 36730606
MH  - *Arabidopsis/genetics/metabolism
MH  - Autophagy/physiology
MH  - Autophagy-Related Proteins/genetics/metabolism
MH  - Endoplasmic Reticulum/metabolism
MH  - *MicroRNAs/metabolism
MH  - *Oxysterols/metabolism
PMC - PMC9618059
OTO - NOTNLM
OT  - PI3P
OT  - autophagy
OT  - membrane contact site
OT  - oxysterol-binding protein-related protein
COIS- The authors declare no competing interest.
EDAT- 2022/10/18 06:00
MHDA- 2022/10/20 06:00
PMCR- 2023/04/17
CRDT- 2022/10/17 15:13
PHST- 2022/10/17 15:13 [entrez]
PHST- 2022/10/18 06:00 [pubmed]
PHST- 2022/10/20 06:00 [medline]
PHST- 2023/04/17 00:00 [pmc-release]
AID - 202205314 [pii]
AID - 10.1073/pnas.2205314119 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2022 Oct 25;119(43):e2205314119. doi: 
      10.1073/pnas.2205314119. Epub 2022 Oct 17.