PMID- 36252660
OWN - NLM
STAT- MEDLINE
DCOM- 20221124
LR  - 20221124
IS  - 1879-1026 (Electronic)
IS  - 0048-9697 (Linking)
VI  - 857
IP  - Pt 2
DP  - 2023 Jan 20
TI  - Tetracycline accumulation in biofilms enhances the selection pressure on 
      Escherichia coli for expression of antibiotic resistance.
PG  - 159441
LID - S0048-9697(22)06540-8 [pii]
LID - 10.1016/j.scitotenv.2022.159441 [doi]
AB  - Microorganisms are present as either biofilm or planktonic species in natural and 
      engineered environments. Little is known about the selection pressure emanating 
      from exposure to sub-minimal inhibitory concentration of antibiotics on 
      planktonic vs. biofilm bacteria. In this study, an E. coli bioreporter was used 
      to develop biofilms on glass and high-density polyethylene (HDPE) surfaces, and 
      compared with the corresponding planktonic bacteria in antibiotic resistance 
      expression when exposed to a range of mug/L levels of tetracycline. The antibiotic 
      resistance-associated fluorescence emissions from biofilm E. coli reached up to 
      1.6 times more than those from planktonic bacteria. The intensively developed 
      biofilms on glass surfaces caused the embedded bacteria to experience higher 
      selection pressure and express more antibiotic resistance than those on HDPE 
      surfaces. The temporal pattern of fluorescence emissions from biofilm E. coli was 
      consistent with the biofilm-developing processes during the experimental period. 
      The increased expression of antibiotic resistance from biofilm bacteria could be 
      attributed to the high affinity of tetracycline with extracellular polymeric 
      substances (EPS). The enhanced accumulation of tetracycline in biofilms could 
      exert higher selection pressure on the embedded bacteria. These results suggest 
      that in many natural and engineered systems the higher antibiotic resistance in 
      biofilm bacteria could be attributed partially to the retention antibiotics by 
      the EPS in biofilms.
CI  - Copyright (c) 2022 Elsevier B.V. All rights reserved.
FAU - Hu, Xiaojie
AU  - Hu X
AD  - Institute of Organic Contaminant Control and Soil Remediation, College of 
      Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, 
      Jiangsu 210095, China; Department of Plant, Soil and Microbial Sciences, Michigan 
      State University, East Lansing, MI 48824, United States.
FAU - Zhang, Yingjie
AU  - Zhang Y
AD  - Department of Plant, Soil and Microbial Sciences, Michigan State University, East 
      Lansing, MI 48824, United States.
FAU - Chen, Zeyou
AU  - Chen Z
AD  - Department of Plant, Soil and Microbial Sciences, Michigan State University, East 
      Lansing, MI 48824, United States.
FAU - Gao, Yanzheng
AU  - Gao Y
AD  - Institute of Organic Contaminant Control and Soil Remediation, College of 
      Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, 
      Jiangsu 210095, China.
FAU - Teppen, Brian
AU  - Teppen B
AD  - Department of Plant, Soil and Microbial Sciences, Michigan State University, East 
      Lansing, MI 48824, United States.
FAU - Boyd, Stephen A
AU  - Boyd SA
AD  - Department of Plant, Soil and Microbial Sciences, Michigan State University, East 
      Lansing, MI 48824, United States.
FAU - Zhang, Wei
AU  - Zhang W
AD  - Department of Plant, Soil and Microbial Sciences, Michigan State University, East 
      Lansing, MI 48824, United States.
FAU - Tiedje, James M
AU  - Tiedje JM
AD  - Department of Plant, Soil and Microbial Sciences, Michigan State University, East 
      Lansing, MI 48824, United States.
FAU - Li, Hui
AU  - Li H
AD  - Department of Plant, Soil and Microbial Sciences, Michigan State University, East 
      Lansing, MI 48824, United States. Electronic address: lihui@msu.edu.
LA  - eng
PT  - Journal Article
DEP - 20221014
PL  - Netherlands
TA  - Sci Total Environ
JT  - The Science of the total environment
JID - 0330500
RN  - 9002-88-4 (Polyethylene)
RN  - F8VB5M810T (Tetracycline)
RN  - 0 (Anti-Bacterial Agents)
SB  - IM
MH  - Humans
MH  - *Escherichia coli
MH  - Polyethylene
MH  - Tetracycline/pharmacology
MH  - Biofilms
MH  - Anti-Bacterial Agents/pharmacology
MH  - *Escherichia coli Infections/microbiology
MH  - Drug Resistance, Microbial
MH  - Bacteria
OTO - NOTNLM
OT  - Antibiotic resistance
OT  - Biofilm
OT  - Extracellular polymeric substances
OT  - Selection pressure
OT  - Tetracycline
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2022/10/18 06:00
MHDA- 2022/11/25 06:00
CRDT- 2022/10/17 19:23
PHST- 2022/08/14 00:00 [received]
PHST- 2022/10/08 00:00 [revised]
PHST- 2022/10/10 00:00 [accepted]
PHST- 2022/10/18 06:00 [pubmed]
PHST- 2022/11/25 06:00 [medline]
PHST- 2022/10/17 19:23 [entrez]
AID - S0048-9697(22)06540-8 [pii]
AID - 10.1016/j.scitotenv.2022.159441 [doi]
PST - ppublish
SO  - Sci Total Environ. 2023 Jan 20;857(Pt 2):159441. doi: 
      10.1016/j.scitotenv.2022.159441. Epub 2022 Oct 14.