PMID- 36255337
OWN - NLM
STAT- MEDLINE
DCOM- 20230712
LR  - 20230718
IS  - 1759-9873 (Electronic)
IS  - 0964-5284 (Linking)
VI  - 41
IP  - 4
DP  - 2023 Aug
TI  - Electroacupuncture alleviates cognitive dysfunction and neuronal pyroptosis in 
      septic mice.
PG  - 246-256
LID - 10.1177/09645284221117847 [doi]
AB  - BACKGROUND: Sepsis is defined as organ dysfunction caused by an uncontrolled 
      response to infection and is followed by a high incidence of cognitive 
      dysfunction, which can severely affect patients' quality of life. Previous 
      studies have suggested that electroacupuncture (EA) is protective against 
      sepsis-associated cognitive dysfunction and that pyroptosis plays a vital role in 
      cognitive function. The aim of this study was to investigate the effect of EA on 
      cognition and neuronal pyroptosis in a mouse model of sepsis. METHODS: Sepsis was 
      induced by cecal ligation and puncture (CLP) surgery. Mice were randomly divided 
      into three groups (control, CLP and CLP + EA). EA was performed at bilateral ST36 
      for three consecutive days after the surgery. The 7-day survival rate of each 
      group was observed on the seventh day after the surgery. The Morris water maze 
      (MWM) was used to test cognitive function from the 8th to 12th day after the 
      surgery. We used transmission electron microscopy (TEM) and transferase dUTP 
      nick-end labeling (TUNEL) staining to determine the structural integrity of 
      hippocampal neuronal membranes and the number of surviving neurons in the 
      hippocampal tissues, respectively. Expression of nucleotide-binding domain-like 
      receptor protein 1 (NLRP1), caspase-1 and gasdermin-D (GSDM D) in hippocampal CA1 
      neurons was detected by Western blotting and real-time fluorescence quantitative 
      polymerase chain reaction (RT-qPCR), and caspase-1 concentrations were measured 
      by enzyme-linked immunosorbent assay. RESULTS: Compared with the CLP group, 7-day 
      survival rates and cognitive function were significantly improved in the CLP + EA 
      group. After EA treatment, the integrity of the hippocampal CA1 neuronal membrane 
      and mortality of hippocampal neurons were significantly decreased, and expression 
      of NLRP1, caspase-1 and GSDM D was downregulated. CONCLUSION: EA can alleviate 
      cognitive dysfunction and neuronal pyroptosis in septic mice.
FAU - Li, Yan
AU  - Li Y
AD  - Department of Anesthesiology, Guizhou Provincial People's Hospital, Guiyang, 
      China.
FAU - Li, Zhaoying
AU  - Li Z
AD  - Department of Anesthesiology, Guizhou Provincial People's Hospital, Guiyang, 
      China.
FAU - He, Fujuan
AU  - He F
AD  - Department of Anesthesiology, Guizhou Provincial People's Hospital, Guiyang, 
      China.
FAU - Qin, Chenguang
AU  - Qin C
AD  - Department of Anesthesiology, Guizhou Provincial People's Hospital, Guiyang, 
      China.
FAU - Fan, Rui
AU  - Fan R
AD  - Department of Anesthesiology, Guizhou Provincial People's Hospital, Guiyang, 
      China.
FAU - Zhang, Fangxiang
AU  - Zhang F
AD  - Department of Anesthesiology, Guizhou Provincial People's Hospital, Guiyang, 
      China.
FAU - Wang, Bin
AU  - Wang B
AD  - Department of Anesthesiology, Guizhou Provincial People's Hospital, Guiyang, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20221018
PL  - England
TA  - Acupunct Med
JT  - Acupuncture in medicine : journal of the British Medical Acupuncture Society
JID - 9304117
RN  - EC 3.4.22.- (Caspases)
SB  - IM
MH  - Mice
MH  - Animals
MH  - Pyroptosis
MH  - *Electroacupuncture
MH  - Quality of Life
MH  - *Cognitive Dysfunction/etiology/therapy/metabolism
MH  - *Sepsis/therapy/metabolism
MH  - Hippocampus/metabolism
MH  - Neurons/metabolism
MH  - Caspases/metabolism
OTO - NOTNLM
OT  - cecal ligation and puncture
OT  - cognitive dysfunction
OT  - electroacupuncture
OT  - neuronal pyroptosis
OT  - sepsis
EDAT- 2022/10/19 06:00
MHDA- 2023/07/12 06:42
CRDT- 2022/10/18 10:22
PHST- 2023/07/12 06:42 [medline]
PHST- 2022/10/19 06:00 [pubmed]
PHST- 2022/10/18 10:22 [entrez]
AID - 10.1177/09645284221117847 [doi]
PST - ppublish
SO  - Acupunct Med. 2023 Aug;41(4):246-256. doi: 10.1177/09645284221117847. Epub 2022 
      Oct 18.