PMID- 36257203
OWN - NLM
STAT- MEDLINE
DCOM- 20221130
LR  - 20221216
IS  - 1878-3279 (Electronic)
IS  - 0171-2985 (Linking)
VI  - 227
IP  - 6
DP  - 2022 Nov
TI  - The utility of inflammatory and endothelial factors in the prognosis of severe 
      dengue.
PG  - 152289
LID - S0171-2985(22)00115-2 [pii]
LID - 10.1016/j.imbio.2022.152289 [doi]
AB  - BACKGROUND: Severe dengue is associated with a considerable risk of mortality, 
      and there is currently a lack of appropriate prognostic biomarkers to predict its 
      severity. Pathogenesis of severe dengue is characterized by overt inflammation, 
      endothelial activation, and increased vascular permeability. The current study 
      investigates the utility of endothelial, inflammatory, and vascular permeability 
      factors as biomarkers to identify dengue severity, which could improve disease 
      prognosis and management. METHODS: The dengue-positive subjects were classified 
      based on seropositivity for NS1, IgM, and IgG. The samples in each group were 
      quantified for basic clinical investigations. The levels of Interleukin-6 (IL-6), 
      Tumor necrosis factor receptor 1 (TNFR1), EOTAXIN, Monocyte chemoattractant 
      protein-1 (MCP-1), Monokine induced by interferon-gamma (MIG), Intercellular 
      adhesion molecule-1 (ICAM-1), Vascular cell adhesion molecule-1 (VCAM-1), 
      Thrombomodulin, and Angiopoietin-2 were estimated in all serum samples using the 
      multiplex bead-based assay. RESULTS: IgG seropositive dengue patients showed 
      abnormal laboratory characteristics and severe dengue symptoms. Among the studied 
      markers, only IL-6, TNFR1, ICAM-1, VCAM-1, Thrombomodulin, and Angiopoietin-2 
      were significantly elevated in IgG seropositive patients compared to healthy 
      controls. Increased IL-6 and TNFR1 levels were associated with decreased platelet 
      count and elevated Hematocrit levels in IgG seropositive patients. Furthermore, 
      ROC curve analysis indicated that IL-6, TNFR1, Thrombomodulin, and Angiopoietin-2 
      showed good potential for predicting dengue severity. CONCLUSION: Inflammatory 
      markers IL-6 and TNFR1, and endothelial factors Angiopoietin-2 and 
      Thrombomodulin, could serve as prognostic markers for severe dengue. These 
      findings also encourage the future study of these biomarkers in the pathogenesis 
      of severe dengue infection.
CI  - Copyright (c) 2022 Elsevier GmbH. All rights reserved.
FAU - Sivasubramanian, Srinivasan
AU  - Sivasubramanian S
AD  - State Level Viral Research and Diagnostic Laboratory (VRDL), Department of 
      Virology, King Institute of Preventive Medicine and Research, Chennai 600 032, 
      India.
FAU - Mohandas, Sundhar
AU  - Mohandas S
AD  - Department of Biotechnology, School of Bioengineering, SRM Institute of Science 
      and Technology, Kattankulathur 603 203, Tamil Nadu, India.
FAU - Gopalan, Vidya
AU  - Gopalan V
AD  - State Level Viral Research and Diagnostic Laboratory (VRDL), Department of 
      Virology, King Institute of Preventive Medicine and Research, Chennai 600 032, 
      India.
FAU - Vimal Raj, Velu
AU  - Vimal Raj V
AD  - State Level Viral Research and Diagnostic Laboratory (VRDL), Department of 
      Virology, King Institute of Preventive Medicine and Research, Chennai 600 032, 
      India.
FAU - Govindan, Karthikeyan
AU  - Govindan K
AD  - State Level Viral Research and Diagnostic Laboratory (VRDL), Department of 
      Virology, King Institute of Preventive Medicine and Research, Chennai 600 032, 
      India.
FAU - Varadarajan, Poovazhagi
AU  - Varadarajan P
AD  - Department of Paediatrics, Institute of Child Health and Hospital for Children, 
      Egmore, Chennai, India.
FAU - Kaveri, Krishnasamy
AU  - Kaveri K
AD  - State Level Viral Research and Diagnostic Laboratory (VRDL), Department of 
      Virology, King Institute of Preventive Medicine and Research, Chennai 600 032, 
      India. Electronic address: kingvdl@gmail.com.
FAU - Ramkumar, Kunka Mohanram
AU  - Ramkumar KM
AD  - Department of Biotechnology, School of Bioengineering, SRM Institute of Science 
      and Technology, Kattankulathur 603 203, Tamil Nadu, India. Electronic address: 
      ramkumak@srmist.edu.in.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20221011
PL  - Netherlands
TA  - Immunobiology
JT  - Immunobiology
JID - 8002742
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - 0 (Angiopoietin-2)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
RN  - 0 (Thrombomodulin)
RN  - 0 (Receptors, Tumor Necrosis Factor, Type I)
RN  - 0 (Interleukin-6)
RN  - 0 (Biomarkers)
RN  - 0 (Immunoglobulin G)
SB  - IM
MH  - Humans
MH  - *Severe Dengue/diagnosis
MH  - Intercellular Adhesion Molecule-1
MH  - Angiopoietin-2
MH  - Vascular Cell Adhesion Molecule-1
MH  - Thrombomodulin
MH  - Receptors, Tumor Necrosis Factor, Type I
MH  - Interleukin-6
MH  - Prognosis
MH  - Biomarkers
MH  - Immunoglobulin G
MH  - *Dengue/diagnosis
OTO - NOTNLM
OT  - Dengue Severity
OT  - Endothelial activation
OT  - Inflammation
OT  - Prognostic markers
OT  - Vascular permeability
COIS- Declaration of Competing Interest The authors declare the following financial 
      interests/personal relationships which may be considered as potential competing 
      interests: Ramkumar KM reports financial support was provided by SRM Institute of 
      Science and Technology.
EDAT- 2022/10/19 06:00
MHDA- 2022/12/01 06:00
CRDT- 2022/10/18 18:24
PHST- 2022/04/02 00:00 [received]
PHST- 2022/09/19 00:00 [revised]
PHST- 2022/10/06 00:00 [accepted]
PHST- 2022/10/19 06:00 [pubmed]
PHST- 2022/12/01 06:00 [medline]
PHST- 2022/10/18 18:24 [entrez]
AID - S0171-2985(22)00115-2 [pii]
AID - 10.1016/j.imbio.2022.152289 [doi]
PST - ppublish
SO  - Immunobiology. 2022 Nov;227(6):152289. doi: 10.1016/j.imbio.2022.152289. Epub 
      2022 Oct 11.