PMID- 36260079
OWN - NLM
STAT- MEDLINE
DCOM- 20221230
LR  - 20230410
IS  - 1460-2202 (Electronic)
IS  - 0271-3683 (Linking)
VI  - 48
IP  - 1
DP  - 2023 Jan
TI  - Circ_0080940 Regulates miR-139-5p/CTGF Pathway to Promote the Proliferation, 
      Migration, Extracellular Matrix Deposition of Human Tenon's Capsule Fibroblasts.
PG  - 34-43
LID - 10.1080/02713683.2022.2138449 [doi]
AB  - PURPOSE: Circular RNA (circRNA) has been identified as an important regulator for 
      glaucoma progression. Our study aims to reveal the circ_0080940 roles in glaucoma 
      progression. METHODS: Transforming growth factor beta1 (TGF-beta1) was used to treat 
      human Tenon's capsule fibroblasts (HTFs) to mimic glaucoma cell models. Cell 
      function was determined by cell counting kit 8 assay, EdU assay and wound healing 
      assay. Protein levels were determined by western blot analysis. Quantitative 
      real-time PCR was used to measure RNA expression. Dual-luciferase reporter assay 
      was performed to evaluate RNA interaction. RESULTS: Our data confirmed that 
      TGF-beta1 induced HTFs proliferation, migration and extracellular matrix (ECM) 
      deposition. Circ_0080940 was highly expressed in glaucoma patients, and its 
      knockdown inhibited TGF-beta1-induced proliferation, migration and ECM deposition in 
      HTFs. Circ_0080940 sponged miR-139-5p, and anti-miR-139-5p revoked the effect of 
      si-circ_0080940 on the biological functions of TGF-beta1-induced HTFs. CTGF was 
      targeted by miR-139-5p, and overexpressed CTGF overturned the inhibition effect 
      of miR-139-5p on the biological functions of TGF-beta1-induced HTFs. Furthermore, 
      CTGF expression could be positively regulated by circ_0080940. CONCLUSION: To sum 
      up, we confirmed that circ_0080940 contributed to glaucoma progression by 
      miR-139-5p/CTGF axis.
FAU - Wang, Yanxi
AU  - Wang Y
AD  - Department of Ophthalmology, the Affiliated Hospital of Southwest Medical 
      University, Luzhou, China.
FAU - Yu, Yinggui
AU  - Yu Y
AD  - Department of Ophthalmology, the Affiliated Hospital of Southwest Medical 
      University, Luzhou, China.
FAU - Xu, Manhua
AU  - Xu M
AD  - Department of Ophthalmology, the Affiliated Hospital of Southwest Medical 
      University, Luzhou, China.
FAU - Zhou, Juan
AU  - Zhou J
AD  - Department of Ophthalmology, the Affiliated Hospital of Southwest Medical 
      University, Luzhou, China.
FAU - Kang, Gangjin
AU  - Kang G
AD  - Department of Ophthalmology, the Affiliated Hospital of Southwest Medical 
      University, Luzhou, China.
FAU - Li, Kaiming
AU  - Li K
AD  - Department of Ophthalmology, the Affiliated Hospital of Southwest Medical 
      University, Luzhou, China.
LA  - eng
PT  - Journal Article
DEP - 20221104
PL  - England
TA  - Curr Eye Res
JT  - Current eye research
JID - 8104312
RN  - 0 (MicroRNAs)
RN  - 0 (MIRN139 microRNA, human)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 0 (RNA, Circular)
RN  - 0 (CCN2 protein, human)
SB  - IM
EIN - Curr Eye Res. 2023 May;48(5):II. PMID: 37032601
MH  - Humans
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Extracellular Matrix/metabolism
MH  - Fibroblasts/metabolism
MH  - Gene Expression Regulation
MH  - *Glaucoma/genetics/metabolism
MH  - *MicroRNAs/metabolism
MH  - *Tenon Capsule/metabolism
MH  - Transforming Growth Factor beta1/pharmacology
MH  - *RNA, Circular/genetics
OTO - NOTNLM
OT  - CTGF
OT  - Glaucoma
OT  - Tenon's capsule fibroblasts
OT  - circ_0080940
OT  - miR-139-5p
EDAT- 2022/10/20 06:00
MHDA- 2022/12/30 06:00
CRDT- 2022/10/19 11:03
PHST- 2022/10/20 06:00 [pubmed]
PHST- 2022/12/30 06:00 [medline]
PHST- 2022/10/19 11:03 [entrez]
AID - 10.1080/02713683.2022.2138449 [doi]
PST - ppublish
SO  - Curr Eye Res. 2023 Jan;48(1):34-43. doi: 10.1080/02713683.2022.2138449. Epub 2022 
      Nov 4.