PMID- 36267809
OWN - NLM
STAT- MEDLINE
DCOM- 20221025
LR  - 20221025
IS  - 1942-0994 (Electronic)
IS  - 1942-0900 (Print)
IS  - 1942-0994 (Linking)
VI  - 2022
DP  - 2022
TI  - circMTND5 Participates in Renal Mitochondrial Injury and Fibrosis by Sponging 
      MIR6812 in Lupus Nephritis.
PG  - 2769487
LID - 10.1155/2022/2769487 [doi]
LID - 2769487
AB  - Recent studies have focused on nuclear-encoded circular RNAs (circRNAs) in kidney 
      diseases, but little is known about mitochondrial circRNAs. Differentially 
      expressed circRNAs were analyzed by RNA deep sequencing from lupus nephritis (LN) 
      biopsies and normal human kidneys. In LN renal biopsies, the most downregulated 
      circRNA was circMTND5, which is encoded in the mitochondrial genome. We 
      quantitated circMTND5 by qPCR and localized by fluorescence in situ hybridization 
      (FISH). Mitochondrial abnormalities were identified by electron microscopy. The 
      expression of mitochondrial genes was decreased, and the expression of 
      profibrotic genes was increased on qPCR and immunostaining. RNA binding sites for 
      MIR6812 and circMTND5 were predicted. MIR6812 expression was increased by FISH 
      and qPCR. In HK-2 cells and its mitochondrial fraction, the role of circMTND5 
      sponging MIR6812 was assessed by their colocalization in mitochondria on FISH, 
      RNA immunoprecipitation, and RNA pulldown coupled with luciferase reporter assay. 
      circMTND5 knockdown upregulated MIR6812, decreased mitochondrial functional gene 
      expression, and increased profibrotic gene expression. Overexpression of 
      circMTND5 reversed these effects in hTGF-beta stimulated HK-2 cells. Similar effects 
      were observed in HK-2 cells with overexpression and with knockdown of MIR6812. We 
      conclude that circMTND5 alleviates renal mitochondrial injury and kidney fibrosis 
      by sponging MIR6812 in LN.
CI  - Copyright (c) 2022 Junjun Luan et al.
FAU - Luan, Junjun
AU  - Luan J
AD  - Department of Nephrology, Shengjing Hospital of China Medical University, 
      Shenyang, China.
FAU - Jiao, Congcong
AU  - Jiao C
AD  - Department of Nephrology, Shengjing Hospital of China Medical University, 
      Shenyang, China.
FAU - Ma, Cong
AU  - Ma C
AD  - Department of Nephrology, Shengjing Hospital of China Medical University, 
      Shenyang, China.
FAU - Zhang, Yixiao
AU  - Zhang Y
AD  - Department of Urology, Shengjing Hospital of China Medical University, Shenyang, 
      China.
FAU - Hao, Xiangnan
AU  - Hao X
AD  - Department of Nephrology, Shengjing Hospital of China Medical University, 
      Shenyang, China.
FAU - Zhou, Guangyu
AU  - Zhou G
AD  - Department of Nephrology, Shengjing Hospital of China Medical University, 
      Shenyang, China.
FAU - Fu, Jingqi
AU  - Fu J
AD  - Program of Environmental Toxicology, School of Public Health, China Medical 
      University, Shenyang, China.
FAU - Qiu, Xingyu
AU  - Qiu X
AD  - Department of Physiology, School of Basic Medical Sciences, Zhejiang University 
      School of Medicine, Hangzhou, China.
FAU - Li, Hongyu
AU  - Li H
AD  - Shensu Science & Technology Co. Ltd., Suzhou, China.
FAU - Yang, Wei
AU  - Yang W
AD  - Shensu Science & Technology Co. Ltd., Suzhou, China.
FAU - Illei, Gabor G
AU  - Illei GG
AD  - Horizon, Gaithersburg, MD, USA.
FAU - Kopp, Jeffrey B
AU  - Kopp JB
AD  - Kidney Disease Section, NIDDK, NIH, Bethesda, USA.
FAU - Pi, Jingbo
AU  - Pi J
AD  - Program of Environmental Toxicology, School of Public Health, China Medical 
      University, Shenyang, China.
FAU - Zhou, Hua
AU  - Zhou H
AUID- ORCID: 0000-0003-0429-116X
AD  - Department of Nephrology, Shengjing Hospital of China Medical University, 
      Shenyang, China.
LA  - eng
PT  - Journal Article
DEP - 20221011
PL  - United States
TA  - Oxid Med Cell Longev
JT  - Oxidative medicine and cellular longevity
JID - 101479826
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Circular)
RN  - EC 1.6.99.3 (MT-ND5 protein, human)
SB  - IM
MH  - Humans
MH  - Fibrosis
MH  - In Situ Hybridization, Fluorescence
MH  - Kidney/pathology
MH  - *Kidney Diseases/genetics/metabolism
MH  - *Lupus Nephritis/genetics/metabolism/pathology
MH  - *MicroRNAs/genetics/metabolism
MH  - Mitochondria/metabolism
MH  - *RNA, Circular/genetics
PMC - PMC9578797
COIS- The authors have no relevant financial or nonfinancial interests to disclose.
EDAT- 2022/10/22 06:00
MHDA- 2022/10/25 06:00
PMCR- 2022/10/11
CRDT- 2022/10/21 03:01
PHST- 2022/05/17 00:00 [received]
PHST- 2022/09/12 00:00 [revised]
PHST- 2022/09/22 00:00 [accepted]
PHST- 2022/10/21 03:01 [entrez]
PHST- 2022/10/22 06:00 [pubmed]
PHST- 2022/10/25 06:00 [medline]
PHST- 2022/10/11 00:00 [pmc-release]
AID - 10.1155/2022/2769487 [doi]
PST - epublish
SO  - Oxid Med Cell Longev. 2022 Oct 11;2022:2769487. doi: 10.1155/2022/2769487. 
      eCollection 2022.