PMID- 36272074
OWN - NLM
STAT- MEDLINE
DCOM- 20221025
LR  - 20221108
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2562
DP  - 2023
TI  - Fluorescence In Situ Hybridization of DNA Probes on Mitotic Chromosomes of the 
      Mexican Axolotl.
PG  - 165-173
LID - 10.1007/978-1-0716-2659-7_10 [doi]
AB  - Fluorescence in situ hybridization (FISH) is used extensively for visual 
      localization of specific DNA fragments (and RNA fragments) in broad applications 
      on chromosomes or nuclei at any stage of the cell cycle: metaphase, anaphase, or 
      interphase. The cytogenetic slides that serve as a target for the labeled DNA 
      probe might be prepared using any approach suitable for obtaining cells with 
      appropriate morphology for imaging and analysis. In this chapter, we focus on the 
      application of molecular cytogenetic methods such as DNA labeling, slide 
      preparation, and in situ hybridization related to cells from Mexican axolotl.
CI  - (c) 2023. The Author(s), under exclusive license to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Keinath, Melissa
AU  - Keinath M
AD  - Carnegie Institute of Science Department of Embryology (Lab 322), Baltimore, MD, 
      USA.
FAU - Timoshevskiy, Vladimir
AU  - Timoshevskiy V
AD  - University of Kentucky, Department of Biology, Lexington, KY, USA. 
      vti224@uky.edu.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (DNA Probes)
RN  - 9007-49-2 (DNA)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Animals
MH  - In Situ Hybridization, Fluorescence/methods
MH  - *Ambystoma mexicanum/genetics
MH  - Interphase/genetics
MH  - *Chromosomes/genetics
MH  - DNA Probes/genetics
MH  - DNA/genetics
MH  - RNA
OTO - NOTNLM
OT  - Axolotl mitotic chromosomes
OT  - BAC
OT  - Chromosomal in situ suppression (CISS) hybridization
OT  - Fluorescence in situ hybridization
OT  - Nick translation
EDAT- 2022/10/23 06:00
MHDA- 2022/10/26 06:00
CRDT- 2022/10/22 11:20
PHST- 2022/10/22 11:20 [entrez]
PHST- 2022/10/23 06:00 [pubmed]
PHST- 2022/10/26 06:00 [medline]
AID - 10.1007/978-1-0716-2659-7_10 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2023;2562:165-173. doi: 10.1007/978-1-0716-2659-7_10.