PMID- 36272481
OWN - NLM
STAT- MEDLINE
DCOM- 20221202
LR  - 20221202
IS  - 1879-1026 (Electronic)
IS  - 0048-9697 (Linking)
VI  - 858
IP  - Pt 1
DP  - 2023 Feb 1
TI  - Early stage biofilm formation on bio-based microplastics in a freshwater 
      reservoir.
PG  - 159569
LID - S0048-9697(22)06668-2 [pii]
LID - 10.1016/j.scitotenv.2022.159569 [doi]
AB  - Bio-based plastics (BP) produced from renewable biomass resources, such as 
      high-density polyethylene (HDPE), polylactic acid (PLA) and 
      poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), is currently increasing in 
      terms of both products and applications. However, their biodegradability and 
      environmental fate are not yet fully understood, especially in freshwaters. Here, 
      we present the results of an in-situ study in a freshwater reservoir, where we 
      submerged HDPE, PLA and PHBV microscale BP (mBP) in dialysis bags to enable 
      exchange of small organic and inorganic molecules, including nutrients, with the 
      surrounding water. After one and two months, the bacterial biofilm that formed on 
      each mBP was characterised by 16S rRNA amplicon sequencing. After two-months, 
      Oxalobacteraceae, Pedosphaeraceae, Flavobacteriaceae (Flavobacterium) and 
      Chitinophagaceae (Ferruginibacter) had increased by up to four times. Both these 
      and other common members (>/=1 % relative total biomass) of the microbial community 
      were similarly abundant on all mBP. Low-abundance (0.3-1 %) bacterial taxa, 
      however, were significantly more diverse and differed on each mBP. Notably, some 
      low-abundance families and genera increased on specific materials, e.g. 
      Sphingomonadaceae on HDPE, Sphingobacteriaceae on PHBV, Gemmatimonas and 
      Crenothrix on PLA. Overall, abundant bacteria were regarded as a pioneering 
      community, while low-abundance bacteria were more diverse and preferred mBP types 
      in the early stages of biofilm formation on mBP. It could be influenced by the 
      environmental conditions, where nutrient levels and low temperatures might shape 
      the low-abundance of attached bacterial communities than the plastic material 
      itself.
CI  - Copyright (c) 2022 Elsevier B.V. All rights reserved.
FAU - Nguyen, Nhung H A
AU  - Nguyen NHA
AD  - Institute for Nanomaterials, Advanced Technologies and Innovation, Technical 
      University of Liberec, Bendlova 1409/7, 461 17 Liberec, Czech Republic. 
      Electronic address: nhung.nguyen@tul.cz.
FAU - Marlita, Marlita
AU  - Marlita M
AD  - Institute for Nanomaterials, Advanced Technologies and Innovation, Technical 
      University of Liberec, Bendlova 1409/7, 461 17 Liberec, Czech Republic; Faculty 
      of Mechatronics, Informatics and Interdisciplinary Studies, Technical University 
      of Liberec, Studentska 1402/2, 461 17 Liberec, Czech Republic.
FAU - El-Temsah, Yehia S
AU  - El-Temsah YS
AD  - Institute for Nanomaterials, Advanced Technologies and Innovation, Technical 
      University of Liberec, Bendlova 1409/7, 461 17 Liberec, Czech Republic; Centre 
      for Biosafety, Postboks 6418, 9294 Tromso, Norway.
FAU - Hrabak, Pavel
AU  - Hrabak P
AD  - Institute for Nanomaterials, Advanced Technologies and Innovation, Technical 
      University of Liberec, Bendlova 1409/7, 461 17 Liberec, Czech Republic; Faculty 
      of Mechatronics, Informatics and Interdisciplinary Studies, Technical University 
      of Liberec, Studentska 1402/2, 461 17 Liberec, Czech Republic.
FAU - Riha, Jakub
AU  - Riha J
AD  - Institute for Nanomaterials, Advanced Technologies and Innovation, Technical 
      University of Liberec, Bendlova 1409/7, 461 17 Liberec, Czech Republic; Faculty 
      of Mechatronics, Informatics and Interdisciplinary Studies, Technical University 
      of Liberec, Studentska 1402/2, 461 17 Liberec, Czech Republic.
FAU - Sevcu, Alena
AU  - Sevcu A
AD  - Institute for Nanomaterials, Advanced Technologies and Innovation, Technical 
      University of Liberec, Bendlova 1409/7, 461 17 Liberec, Czech Republic; Faculty 
      of Science, Humanities and Education, Technical University of Liberec, Studentska 
      1402/2, 461 17 Liberec, Czech Republic. Electronic address: alena.sevcu@tul.cz.
LA  - eng
PT  - Journal Article
DEP - 20221020
PL  - Netherlands
TA  - Sci Total Environ
JT  - The Science of the total environment
JID - 0330500
RN  - 0 (Microplastics)
RN  - 0 (Plastics)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 9002-88-4 (Polyethylene)
RN  - 0 (Polyesters)
RN  - 10237-77-1 (beta-hydroxyvaleric acid)
SB  - IM
MH  - Humans
MH  - *Microplastics
MH  - *Plastics
MH  - RNA, Ribosomal, 16S/genetics
MH  - Polyethylene
MH  - Renal Dialysis
MH  - Fresh Water
MH  - Biofilms
MH  - Bacteria
MH  - Polyesters
OTO - NOTNLM
OT  - Abundant bacteria
OT  - HDPE
OT  - In-situ
OT  - Low-abundance bacteria
OT  - PHBV
OT  - PLA
COIS- Declaration of competing interest The authors declare no financial competition or 
      competing interests in relation to the work described.
EDAT- 2022/10/23 06:00
MHDA- 2022/12/03 06:00
CRDT- 2022/10/22 19:23
PHST- 2022/09/02 00:00 [received]
PHST- 2022/10/14 00:00 [revised]
PHST- 2022/10/15 00:00 [accepted]
PHST- 2022/10/23 06:00 [pubmed]
PHST- 2022/12/03 06:00 [medline]
PHST- 2022/10/22 19:23 [entrez]
AID - S0048-9697(22)06668-2 [pii]
AID - 10.1016/j.scitotenv.2022.159569 [doi]
PST - ppublish
SO  - Sci Total Environ. 2023 Feb 1;858(Pt 1):159569. doi: 
      10.1016/j.scitotenv.2022.159569. Epub 2022 Oct 20.