PMID- 36274203
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221029
IS  - 2228-5806 (Print)
IS  - 2228-5814 (Electronic)
IS  - 2228-5806 (Linking)
VI  - 24
IP  - 9
DP  - 2022 Sep 12
TI  - An Effective Method for Decellularization of Human Foreskin: Implications for 
      Skin Regeneration in Small Wounds.
PG  - 506-514
LID - 253340 [pii]
LID - 10.22074/cellj.2022.8005 [doi]
AB  - OBJECTIVE: Acellular matrices of different allogeneic or xenogeneic origins are 
      widely used as structural scaffolds in regenerative medicine. The main goal of 
      this research was to optimize a method for decellularization of foreskin for skin 
      regeneration in small wounds. MATERIALS AND METHODS: In this experimental study, 
      the dermal layers of foreskin were divided into two sections and subjected to two 
      different decellularization methods: the sodium dodecyl sulfate method (SDS-M), 
      and our optimized foreskin decellularization method (OFD-M). A combination of 
      non-ionic detergents and SDS were used to decellularize the foreskin in OFD-M. 
      The histological, morphological, and biomechanical properties of both methods 
      were compared. In addition, human umbilical cord mesenchymal stem cells (hucMSCs) 
      were isolated, and the biocompatibility and recellularization of both scaffolds 
      by hucMSC were subsequently determined. RESULTS: We observed that OFD-M is an 
      appropriate approach for successful removal of cellular components from the 
      foreskin tissue, without physical disturbance to the acellular matrix. In 
      comparison to SDS-M, this new bioscaffold possesses a fine network containing a 
      high amount of collagen fibers and glycosaminoglycans (GAG) (P</=0.03), is 
      biocompatible and harmless for hucMSC (viability 91.7%), and exhibits a 
      relatively high tensile strength. CONCLUSION: We found that the extracellular 
      matrix (ECM) structural integrity, the main ECM components, and the mechanical 
      properties of the foreskin are well maintained after applying the OFD-M 
      decellularization technique, indicating that the resulting scaffold would be a 
      suitable platform for culturing MSC for skin grafting in small wounds.
FAU - Rahmati, Shima
AU  - Rahmati S
AD  - Cancer and Immunology Research Center, Research Institute for Health Development, 
      Kurdistan University of Medical Sciences, Sanandaj, Iran.
FAU - Jalili, Ali
AU  - Jalili A
AD  - Cancer and Immunology Research Center, Research Institute for Health Development, 
      Kurdistan University of Medical Sciences, Sanandaj, Iran.
FAU - Dehkordi, Mehdi Banitalebi
AU  - Dehkordi MB
AD  - Department of Molecular Medicine, School of Advanced Technologies, Shahrekord 
      University of Medical Sciences, Shahrekord, Iran.
FAU - Przedborski, Michelle
AU  - Przedborski M
AD  - Department of Applied Mathematics, University of Waterloo, Waterloo, ON, Canada.
LA  - eng
PT  - Journal Article
DEP - 20220912
PL  - Iran
TA  - Cell J
JT  - Cell journal
JID - 101566618
PMC - PMC9588162
OTO - NOTNLM
OT  - Decellularized Scaffolds
OT  - Foreskin
OT  - Mesenchymal stem cell
EDAT- 2022/10/25 06:00
MHDA- 2022/10/25 06:01
PMCR- 2022/09/01
CRDT- 2022/10/24 00:42
PHST- 2021/03/03 00:00 [received]
PHST- 2022/10/24 00:42 [entrez]
PHST- 2022/10/25 06:00 [pubmed]
PHST- 2022/10/25 06:01 [medline]
PHST- 2022/09/01 00:00 [pmc-release]
AID - 253340 [pii]
AID - 10.22074/cellj.2022.8005 [doi]
PST - epublish
SO  - Cell J. 2022 Sep 12;24(9):506-514. doi: 10.22074/cellj.2022.8005.