PMID- 36274817
OWN - NLM
STAT- MEDLINE
DCOM- 20221026
LR  - 20221031
IS  - 1090-0535 (Electronic)
IS  - 1090-0535 (Linking)
VI  - 28
DP  - 2022
TI  - Dopachrome tautomerase is a retinoblastoma-specific gene, and its proximal 
      promoter is preferentially active in human retinoblastoma cells.
PG  - 192-202
AB  - PURPOSE: Retinoblastoma (RB) is a malignant childhood intraocular tumor. Current 
      treatment options for RB have undesirable side effects. A comprehensive 
      understanding of gene expression in human RB is essential for the development of 
      safe and effective new therapies. METHODS: We reviewed published microarray and 
      RNA sequencing studies in which gene expression profiles were compared between 
      human RB and normal retina tissues. We investigated the expression of genes of 
      interest using quantitative reverse transcription PCR. We examined the activities 
      of cloned promoter DNA fragments with luciferase assay. RESULTS: Dopachrome 
      tautomerase (DCT) was among the most overexpressed genes in RB in published 
      studies. We found that DCT was highly expressed in six of 13 samples 
      microdissected from Thai RB tissues. Expression of DCT was absent or barely 
      detected in retina tissues, various human ocular cells, and major organs. We also 
      demonstrated that the -657 to +411 DCT promoter fragment efficiently directs RB 
      cell-specific transcription of the luciferase reporter gene in cell lines. 
      CONCLUSIONS: The present work highlights that DCT is one of the most RB-specific 
      genes. The regulatory elements required for this cell-specific gene expression 
      are likely located within its proximal promoter.
CI  - Copyright (c) 2022 Molecular Vision.
FAU - Moolsuwan, Kanya
AU  - Moolsuwan K
AD  - Graduate Program in Molecular Medicine, Faculty of Science, Mahidol University, 
      Bangkok, Thailand.
FAU - Permpoon, Tiravut
AU  - Permpoon T
AD  - Clinical Molecular Pathology Laboratory, Department of Clinical Pathology, 
      Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
FAU - Sae-Lee, Chanachai
AU  - Sae-Lee C
AD  - Research Division, Faculty of Medicine Siriraj Hospital, Mahidol University, 
      Bangkok, Thailand.
FAU - Uiprasertkul, Mongkol
AU  - Uiprasertkul M
AD  - Department of Pathology, Faculty of Medicine Siriraj Hospital, Mahidol 
      University, Bangkok, Thailand.
FAU - Boonyaratanakornkit, Viroj
AU  - Boonyaratanakornkit V
AD  - Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand.
FAU - Yenchitsomanus, Pa-Thai
AU  - Yenchitsomanus PT
AD  - Division of Molecular Medicine, Department of Research and Development, Faculty 
      of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
FAU - Poungvarin, Naravat
AU  - Poungvarin N
AD  - Clinical Molecular Pathology Laboratory, Department of Clinical Pathology, 
      Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
FAU - Atchaneeyasakul, La-Ongsri
AU  - Atchaneeyasakul LO
AD  - Department of Ophthalmology, Faculty of Medicine Siriraj Hospital, Mahidol 
      University, Bangkok, Thailand.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
DEP - 20220807
PL  - United States
TA  - Mol Vis
JT  - Molecular vision
JID - 9605351
RN  - EC 5.3.3.12 (dopachrome isomerase)
RN  - EC 5.3.- (Intramolecular Oxidoreductases)
SB  - IM
MH  - Child
MH  - Humans
MH  - Gene Expression Regulation, Neoplastic
MH  - Genes, Retinoblastoma/genetics
MH  - *Intramolecular Oxidoreductases/genetics/metabolism
MH  - *Retinal Neoplasms/genetics
MH  - *Retinoblastoma/genetics/pathology
PMC - PMC9491246
EDAT- 2022/10/25 06:00
MHDA- 2022/10/26 06:00
PMCR- 2022/01/01
CRDT- 2022/10/24 03:47
PHST- 2021/11/11 00:00 [received]
PHST- 2022/08/05 00:00 [accepted]
PHST- 2022/10/24 03:47 [entrez]
PHST- 2022/10/25 06:00 [pubmed]
PHST- 2022/10/26 06:00 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 17 [pii]
PST - epublish
SO  - Mol Vis. 2022 Aug 7;28:192-202. eCollection 2022.