PMID- 36275018
OWN - NLM
STAT- MEDLINE
DCOM- 20221025
LR  - 20221222
IS  - 2235-2988 (Electronic)
IS  - 2235-2988 (Linking)
VI  - 12
DP  - 2022
TI  - Immunological evaluation of recombination PRRSV GP3 and GP5 DNA vaccines in vivo.
PG  - 1016897
LID - 10.3389/fcimb.2022.1016897 [doi]
LID - 1016897
AB  - The porcine reproductive and respiratory syndrome virus (PRRSV) is a threat to 
      the health of pigs worldwide, but commercially available vaccines offer limited 
      protection against PRRSV infection. It is necessary to develop a more effective 
      DNA vaccine. The immunological effects of DNA vaccines with three adjuvants were 
      examined in pigs (Susscrofa domestica) challenged with PRRSV. These DNA vaccines, 
      which encoded PRRSV GP3 and GP5, were formulated with A1, A2, and A3. Serum 
      specific and neutralizing antibodies, IL-4, IFN-gamma, IL-2, IL-10, CD4(+) and 
      CD8(+)T-lymphocytes, health status, histopathology, and viral loads were 
      determined. The results showed that the use of adjuvant A3 led to higher levels 
      of neutralizing antibodies and a lower viral load in pigs compared to the other 
      adjuvants. The neutralizing antibody titers of the pVAX-GP35+A1 and pVAX-GP35+A3 
      groups reached a peak of 1:19 at 35 dpi. The maximum concentration of IL-4 was 
      136.77 pg/mL in the pVAX-GP35+A3 group. At 35 dpi, the IFN-gamma concentration in the 
      pVAX-GP35+A1 group was 227.4 pg/mL. pVAX-GP35+A3 group shows the highest IL-2 and 
      IL-10 expression to the peak of 597.6 pg/mL and 189.1 pg/mL, respectively. We 
      found a formulation demonstrated beneficial immune outcomes. This study provides 
      an alternative vaccine to protect pigs from PRRSV.
CI  - Copyright (c) 2022 Zhao, Zhang, Sun, Xie, Zhang, Gao, Wen, Ha, Nan, Zhu, Feng, Cao, 
      Zhang, Zhu, Jin and Lu.
FAU - Zhao, Guanyu
AU  - Zhao G
AD  - College of Veterinary Medicine, College of Animal Science, Jilin University, 
      Changchun, China.
FAU - Zhang, Jiaqi
AU  - Zhang J
AD  - Changchun Veterinary Research Institute, Chinese Academy of Agricultural 
      Sciences, Changchun, China.
FAU - Sun, Wenchao
AU  - Sun W
AD  - Changchun Veterinary Research Institute, Chinese Academy of Agricultural 
      Sciences, Changchun, China.
FAU - Xie, Changzhan
AU  - Xie C
AD  - Changchun Veterinary Research Institute, Chinese Academy of Agricultural 
      Sciences, Changchun, China.
FAU - Zhang, He
AU  - Zhang H
AD  - Changchun Veterinary Research Institute, Chinese Academy of Agricultural 
      Sciences, Changchun, China.
FAU - Gao, Yan
AU  - Gao Y
AD  - Changchun Veterinary Research Institute, Chinese Academy of Agricultural 
      Sciences, Changchun, China.
FAU - Wen, Shubo
AU  - Wen S
AD  - Changchun Veterinary Research Institute, Chinese Academy of Agricultural 
      Sciences, Changchun, China.
FAU - Ha, Zhuo
AU  - Ha Z
AD  - Changchun Veterinary Research Institute, Chinese Academy of Agricultural 
      Sciences, Changchun, China.
FAU - Nan, Fulong
AU  - Nan F
AD  - Changchun Veterinary Research Institute, Chinese Academy of Agricultural 
      Sciences, Changchun, China.
FAU - Zhu, Xiangyu
AU  - Zhu X
AD  - Changchun Veterinary Research Institute, Chinese Academy of Agricultural 
      Sciences, Changchun, China.
FAU - Feng, Sheng
AU  - Feng S
AD  - Changchun Veterinary Research Institute, Chinese Academy of Agricultural 
      Sciences, Changchun, China.
FAU - Cao, Xinyu
AU  - Cao X
AD  - Changchun Veterinary Research Institute, Chinese Academy of Agricultural 
      Sciences, Changchun, China.
FAU - Zhang, Ying
AU  - Zhang Y
AD  - College of Veterinary Medicine, College of Animal Science, Jilin University, 
      Changchun, China.
FAU - Zhu, Yanzhu
AU  - Zhu Y
AD  - Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural 
      Sciences, Changchun, China.
AD  - Animal Science and Technology College, Jilin Agriculture Science and Technology 
      University, Jilin, China.
FAU - Jin, Ningyi
AU  - Jin N
AD  - College of Veterinary Medicine, College of Animal Science, Jilin University, 
      Changchun, China.
AD  - Changchun Veterinary Research Institute, Chinese Academy of Agricultural 
      Sciences, Changchun, China.
FAU - Lu, Huijun
AU  - Lu H
AD  - Changchun Veterinary Research Institute, Chinese Academy of Agricultural 
      Sciences, Changchun, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20221006
PL  - Switzerland
TA  - Front Cell Infect Microbiol
JT  - Frontiers in cellular and infection microbiology
JID - 101585359
RN  - 0 (Vaccines, DNA)
RN  - 130068-27-8 (Interleukin-10)
RN  - 207137-56-2 (Interleukin-4)
RN  - 0 (Interleukin-2)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Antibodies, Neutralizing)
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Swine
MH  - Animals
MH  - *Porcine respiratory and reproductive syndrome virus/genetics
MH  - *Vaccines, DNA
MH  - *Porcine Reproductive and Respiratory Syndrome/prevention & control
MH  - Interleukin-10/genetics
MH  - Interleukin-4
MH  - Interleukin-2/genetics
MH  - Antibodies, Viral
MH  - Antibodies, Neutralizing
MH  - Adjuvants, Immunologic/pharmacology
MH  - Recombination, Genetic
MH  - *Viral Vaccines/genetics
PMC - PMC9582230
OTO - NOTNLM
OT  - DNA vaccine
OT  - GP3
OT  - GP5
OT  - PRRSV
OT  - immune response
OT  - pig
COIS- The authors state that there were no commercial or financial relationships that 
      may be considered as a potential conflict of interest during the research.
EDAT- 2022/10/25 06:00
MHDA- 2022/10/26 06:00
PMCR- 2022/01/01
CRDT- 2022/10/24 03:51
PHST- 2022/08/11 00:00 [received]
PHST- 2022/09/05 00:00 [accepted]
PHST- 2022/10/24 03:51 [entrez]
PHST- 2022/10/25 06:00 [pubmed]
PHST- 2022/10/26 06:00 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 10.3389/fcimb.2022.1016897 [doi]
PST - epublish
SO  - Front Cell Infect Microbiol. 2022 Oct 6;12:1016897. doi: 
      10.3389/fcimb.2022.1016897. eCollection 2022.