PMID- 36279394
OWN - NLM
STAT- MEDLINE
DCOM- 20221108
LR  - 20221123
IS  - 1945-4589 (Electronic)
IS  - 1945-4589 (Linking)
VI  - 14
IP  - 20
DP  - 2022 Oct 20
TI  - Network analysis for the identification of hub genes and related molecules as 
      potential biomarkers associated with the differentiation of bone marrow-derived 
      stem cells into hepatocytes.
PG  - 8243-8257
LID - 10.18632/aging.204344 [doi]
AB  - The incidence of liver diseases has been increasing steadily. However, it has 
      some shortcomings, such as high cost and organ donor scarcity. The application of 
      stem cell research has brought new ideas for the treatment of liver diseases. 
      Therefore, it is particularly important to clarify the molecular and regulatory 
      mechanisms of differentiation of bone marrow-derived stem cells (BMSCs) into 
      liver cells. Herein, we screened differentially expressed genes between 
      hepatocytes and untreated BMSCs to identify the genes responsible for the 
      differentiation of BMSCs into hepatocytes. GSE30419 gene microarray data of BMSCs 
      and GSE72088 gene microarray data of primary hepatocytes were obtained from the 
      Gene Expression Omnibus database. Transcriptome Analysis Console software showed 
      that 1896 genes were upregulated and 2506 were downregulated in hepatocytes as 
      compared with BMSCs. Hub genes were analyzed using the STRING and Cytoscape v 
      3.8.2, revealing that twenty-four hub genes, play a pivotal role in the 
      differentiation of BMSCs into hepatocytes. The expression of the hub genes in the 
      BMSCs and hepatocytes was verified by reverse transcription-quantitative PCR 
      (RT-qPCR). Next, the target miRNAs of hub genes were predicted, and then the 
      lncRNAs regulating miRNAs was discovered, thus forming the lncRNA-miRNA-mRNA 
      interaction chain. The results indicate that the lncRNA-miRNA-mRNA interaction 
      chain may play an important role in the differentiation of BMSCs into 
      hepatocytes, which provides a new therapeutic target for liver disease treatment.
FAU - Han, Ying-Hao
AU  - Han YH
AD  - College of Life Science and Technology, Heilongjiang Bayi Agricultural 
      University, Daqing 163319, Heilongjiang, P.R. China.
FAU - He, Xin-Mei
AU  - He XM
AD  - College of Life Science and Technology, Heilongjiang Bayi Agricultural 
      University, Daqing 163319, Heilongjiang, P.R. China.
FAU - Lee, Seung-Jae
AU  - Lee SJ
AD  - Functional Biomaterial Research Center, Korea Research Institute of Bioscience 
      and Biotechnology, Jeongeup-Si 56212, Jeonbuk, Republic of Korea.
AD  - Department of Applied Biological Engineering, KRIBB School of Biotechnology, 
      University of Science and Technology, Daejeon 34113, Republic of Korea.
FAU - Mao, Ying-Ying
AU  - Mao YY
AD  - College of Life Science and Technology, Heilongjiang Bayi Agricultural 
      University, Daqing 163319, Heilongjiang, P.R. China.
FAU - Liu, Xuan-Chen
AU  - Liu XC
AD  - College of Life Science and Technology, Heilongjiang Bayi Agricultural 
      University, Daqing 163319, Heilongjiang, P.R. China.
FAU - Sun, Hu-Nan
AU  - Sun HN
AD  - College of Life Science and Technology, Heilongjiang Bayi Agricultural 
      University, Daqing 163319, Heilongjiang, P.R. China.
FAU - Jin, Mei-Hua
AU  - Jin MH
AD  - College of Life Science and Technology, Heilongjiang Bayi Agricultural 
      University, Daqing 163319, Heilongjiang, P.R. China.
FAU - Kwon, Taeho
AU  - Kwon T
AD  - Primate Resources Center, Korea Research Institute of Bioscience and 
      Biotechnology (KRIBB), Jeongeup-Si 56216, Jeonbuk, Republic of Korea.
AD  - Department of Functional Genomics, KRIBB School of Bioscience, University of 
      Science and Technology, Daejeon 34113, Republic of Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20221020
PL  - United States
TA  - Aging (Albany NY)
JT  - Aging
JID - 101508617
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (RNA, Messenger)
RN  - 0 (MicroRNAs)
RN  - 0 (Biomarkers)
SB  - IM
MH  - *RNA, Long Noncoding/genetics
MH  - Bone Marrow/metabolism
MH  - RNA, Messenger/metabolism
MH  - *MicroRNAs/genetics/metabolism
MH  - Hepatocytes/metabolism
MH  - Biomarkers
MH  - Stem Cells/metabolism
MH  - Gene Regulatory Networks
PMC - PMC9648814
OTO - NOTNLM
OT  - BMSCs
OT  - hepatocytes
OT  - hub genes
OT  - lncRNA
OT  - miRNA
COIS- CONFLICTS OF INTEREST: The authors declare no conflicts of interest related to 
      this study.
EDAT- 2022/10/25 06:00
MHDA- 2022/11/09 06:00
PMCR- 2022/10/31
CRDT- 2022/10/24 14:22
PHST- 2022/05/13 00:00 [received]
PHST- 2022/10/05 00:00 [accepted]
PHST- 2022/10/25 06:00 [pubmed]
PHST- 2022/11/09 06:00 [medline]
PHST- 2022/10/24 14:22 [entrez]
PHST- 2022/10/31 00:00 [pmc-release]
AID - 204344 [pii]
AID - 10.18632/aging.204344 [doi]
PST - ppublish
SO  - Aging (Albany NY). 2022 Oct 20;14(20):8243-8257. doi: 10.18632/aging.204344. Epub 
      2022 Oct 20.