PMID- 36281753
OWN - NLM
STAT- MEDLINE
DCOM- 20230517
LR  - 20230522
IS  - 2326-5205 (Electronic)
IS  - 2326-5191 (Linking)
VI  - 75
IP  - 5
DP  - 2023 May
TI  - Identification of a Distinct Monocyte-Driven Signature in Systemic Sclerosis 
      Using Biophysical Phenotyping of Circulating Immune Cells.
PG  - 768-781
LID - 10.1002/art.42394 [doi]
AB  - OBJECTIVE: Pathologically activated circulating immune cells, including 
      monocytes, play major roles in systemic sclerosis (SSc). Their functional 
      characterization can provide crucial information with direct clinical relevance. 
      However, tools for the evaluation of pathologic immune cell activation and, in 
      general, of clinical outcomes in SSc are scarce. Biophysical phenotyping 
      (including characterization of cell mechanics and morphology) provides access to 
      a novel, mostly unexplored layer of information regarding pathophysiologic immune 
      cell activation. We hypothesized that the biophysical phenotyping of circulating 
      immune cells, reflecting their pathologic activation, can be used as a clinical 
      tool for the evaluation and risk stratification of patients with SSc. METHODS: We 
      performed biophysical phenotyping of circulating immune cells by real-time 
      fluorescence and deformability cytometry (RT-FDC) in 63 SSc patients, 59 
      rheumatoid arthritis (RA) patients, 28 antineutrophil cytoplasmic 
      antibody-associated vasculitis (AAV) patients, and 22 age- and sex-matched 
      healthy donors. RESULTS: We identified a specific signature of biophysical 
      properties of circulating immune cells in SSc patients that was mainly driven by 
      monocytes. Since it is absent in RA and AAV, this signature reflects an 
      SSc-specific monocyte activation rather than general inflammation. The 
      biophysical properties of monocytes indicate current disease activity, the extent 
      of skin or lung fibrosis, and the severity of manifestations of microvascular 
      damage, as well as the risk of disease progression in SSc patients. CONCLUSION: 
      Changes in the biophysical properties of circulating immune cells reflect their 
      pathologic activation in SSc patients and are associated with clinical outcomes. 
      As a high-throughput approach that requires minimal preparations, RT-FDC-based 
      biophysical phenotyping of monocytes can serve as a tool for the evaluation and 
      risk stratification of patients with SSc.
CI  - (c) 2022 The Authors. Arthritis & Rheumatology published by Wiley Periodicals LLC 
      on behalf of American College of Rheumatology.
FAU - Matei, Alexandru-Emil
AU  - Matei AE
AUID- ORCID: 0000-0003-1248-3145
AD  - Department of Rheumatology and Hiller Research Unit, University Hospital 
      Dusseldorf, Medical Faculty of Heinrich Heine University, Dusseldorf, Germany, 
      and Department of Internal Medicine 3-Rheumatology and Immunology and Deutsches 
      Zentrum Immuntherapie (DZI), Friedrich-Alexander University Erlangen-Nurnberg 
      (FAU), and Universitatsklinikum Erlangen, Erlangen, Germany.
FAU - Kubankova, Marketa
AU  - Kubankova M
AD  - Max Planck Institute for the Science of Light & Max-Planck-Center fur Physik und 
      Medizin, Erlangen, Germany, and Biotechnology Center, Center for Molecular and 
      Cellular Bioengineering, Technische Universitat Dresden, Dresden, Germany.
FAU - Xu, Liyan
AU  - Xu L
AD  - Department of Rheumatology and Hiller Research Unit, University Hospital 
      Dusseldorf, Medical Faculty of Heinrich Heine University, Dusseldorf, Germany, 
      and Department of Internal Medicine 3-Rheumatology and Immunology and Deutsches 
      Zentrum Immuntherapie (DZI), Friedrich-Alexander University Erlangen-Nurnberg 
      (FAU), and Universitatsklinikum Erlangen, Erlangen, Germany.
FAU - Gyorfi, Andrea-Hermina
AU  - Gyorfi AH
AD  - Department of Rheumatology and Hiller Research Unit, University Hospital 
      Dusseldorf, Medical Faculty of Heinrich Heine University, Dusseldorf, Germany, 
      and Department of Internal Medicine 3-Rheumatology and Immunology and Deutsches 
      Zentrum Immuntherapie (DZI), Friedrich-Alexander University Erlangen-Nurnberg 
      (FAU), and Universitatsklinikum Erlangen, Erlangen, Germany.
FAU - Boxberger, Evgenia
AU  - Boxberger E
AD  - Department of Internal Medicine 3-Rheumatology and Immunology and Deutsches 
      Zentrum Immuntherapie (DZI), Friedrich-Alexander-University Erlangen-Nurnberg 
      (FAU) and Universitatsklinikum Erlangen, Erlangen, Germany.
FAU - Soteriou, Despina
AU  - Soteriou D
AD  - Max Planck Institute for the Science of Light & Max-Planck-Center fur Physik und 
      Medizin, Erlangen, Germany.
FAU - Papava, Maria
AU  - Papava M
AD  - Department of Internal Medicine 3-Rheumatology and Immunology and Deutsches 
      Zentrum Immuntherapie (DZI), Friedrich-Alexander-University Erlangen-Nurnberg 
      (FAU) and Universitatsklinikum Erlangen, Erlangen, Germany.
FAU - Prater, Julia
AU  - Prater J
AD  - Department of Internal Medicine 3-Rheumatology and Immunology and Deutsches 
      Zentrum Immuntherapie (DZI), Friedrich-Alexander-University Erlangen-Nurnberg 
      (FAU) and Universitatsklinikum Erlangen, Erlangen, Germany.
FAU - Hong, Xuezhi
AU  - Hong X
AD  - Department of Rheumatology and Hiller Research Unit, University Hospital 
      Dusseldorf, Medical Faculty of Heinrich Heine University, Dusseldorf, Germany, 
      and Department of Internal Medicine 3-Rheumatology and Immunology and Deutsches 
      Zentrum Immuntherapie (DZI), Friedrich-Alexander University Erlangen-Nurnberg 
      (FAU), and Universitatsklinikum Erlangen, Erlangen, Germany.
FAU - Bergmann, Christina
AU  - Bergmann C
AUID- ORCID: 0000-0001-5257-9171
AD  - Department of Internal Medicine 3-Rheumatology and Immunology and Deutsches 
      Zentrum Immuntherapie (DZI), Friedrich-Alexander-University Erlangen-Nurnberg 
      (FAU) and Universitatsklinikum Erlangen, Erlangen, Germany.
FAU - Krater, Martin
AU  - Krater M
AD  - Max Planck Institute for the Science of Light & Max-Planck-Center fur Physik und 
      Medizin, Erlangen, Germany, and Biotechnology Center, Center for Molecular and 
      Cellular Bioengineering, Technische Universitat Dresden, Dresden, Germany.
FAU - Schett, Georg
AU  - Schett G
AUID- ORCID: 0000-0001-8740-9615
AD  - Department of Internal Medicine 3-Rheumatology and Immunology and Deutsches 
      Zentrum Immuntherapie (DZI), Friedrich-Alexander-University Erlangen-Nurnberg 
      (FAU) and Universitatsklinikum Erlangen, Erlangen, Germany.
FAU - Guck, Jochen
AU  - Guck J
AD  - Max Planck Institute for the Science of Light & Max-Planck-Center fur Physik und 
      Medizin, Erlangen, Germany, and Biotechnology Center, Center for Molecular and 
      Cellular Bioengineering, Technische Universitat Dresden, Dresden, Germany.
FAU - Distler, Jorg H W
AU  - Distler JHW
AUID- ORCID: 0000-0001-7408-9333
AD  - Department of Rheumatology and Hiller Research Unit, University Hospital 
      Dusseldorf, Medical Faculty of Heinrich Heine University, Dusseldorf, Germany, 
      and Department of Internal Medicine 3-Rheumatology and Immunology and Deutsches 
      Zentrum Immuntherapie (DZI), Friedrich-Alexander University Erlangen-Nurnberg 
      (FAU), and Universitatsklinikum Erlangen, Erlangen, Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230320
PL  - United States
TA  - Arthritis Rheumatol
JT  - Arthritis & rheumatology (Hoboken, N.J.)
JID - 101623795
SB  - IM
MH  - Humans
MH  - Monocytes
MH  - *Scleroderma, Systemic/complications
MH  - *Pulmonary Fibrosis/pathology
MH  - Skin/pathology
MH  - *Arthritis, Rheumatoid
EDAT- 2022/10/26 06:00
MHDA- 2023/05/17 06:42
CRDT- 2022/10/25 04:53
PHST- 2022/09/08 00:00 [revised]
PHST- 2022/02/02 00:00 [received]
PHST- 2022/10/18 00:00 [accepted]
PHST- 2023/05/17 06:42 [medline]
PHST- 2022/10/26 06:00 [pubmed]
PHST- 2022/10/25 04:53 [entrez]
AID - 10.1002/art.42394 [doi]
PST - ppublish
SO  - Arthritis Rheumatol. 2023 May;75(5):768-781. doi: 10.1002/art.42394. Epub 2023 
      Mar 20.