PMID- 36282470
OWN - NLM
STAT- MEDLINE
DCOM- 20230621
LR  - 20230621
IS  - 1559-0720 (Electronic)
IS  - 0163-4984 (Linking)
VI  - 201
IP  - 8
DP  - 2023 Aug
TI  - Short-term Dietary Selenium Deficiency Induced Liver Fibrosis by Inhibiting the 
      Akt/mTOR Signaling Pathway in Rats.
PG  - 3825-3833
LID - 10.1007/s12011-022-03453-7 [doi]
AB  - The effects of short-term dietary selenium deficiency on the liver and protein 
      kinase B (Akt)/mammalian target of rapamycin (mTOR) signaling pathway were 
      evaluated. Fourteen growing rats were randomly divided into control and selenium 
      deficiency groups and fed standard and selenium-deficient diets for 4 weeks, 
      respectively. The serum and liver selenium concentrations were measured to 
      evaluate the construction of animal models with selenium deficiency. Liver 
      tissues were analyzed by transmission electron microscope, hematoxylin-eosin 
      staining, and Masson staining to observe the ultrastructural changes, 
      pathological changes, and severity of liver fibrosis, respectively. Besides, 
      immunohistochemical staining (IHC) was used to analyze the effects of selenium 
      deficiency on the expression of key proteins in the Akt/mTOR signaling pathway. 
      The results showed that selenium concentrations in the serum and liver tissue 
      were significantly lower in the selenium deficiency group than in the control 
      group, and the selenium deficiency intervention could affect the morphology and 
      structure of hepatocytes and mitochondria. Meanwhile, the liver tissue showed 
      structural damage and fibrotic changes in the selenium deficiency group. The IHC 
      results showed the positive staining rates of Akt, phosphorylation-modified 
      protein kinase B (p-Akt), mTOR, and phosphorylation-modified mammalian target of 
      the rapamycin (p-mTOR) in the liver of the selenium deficiency group which were 
      significantly lower than that of the control group. In conclusion, short-term 
      selenium deficiency dietary intervention could lead to liver fibrosis by 
      inhibiting the Akt/mTOR signaling pathway.
CI  - (c) 2022. The Author(s), under exclusive licence to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Qiao, Lichun
AU  - Qiao L
AUID- ORCID: 0000-0002-6739-0846
AD  - Department of Occupational and Environmental Health, School of Public Health, 
      Health Science Center, Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, China.
AD  - Global Health Institute, Health Science Center, Xi'an Jiaotong University, Xi'an, 
      712000, Shaanxi, China.
FAU - Lin, Xue
AU  - Lin X
AUID- ORCID: 0000-0003-4465-7007
AD  - Department of Occupational and Environmental Health, School of Public Health, 
      Health Science Center, Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, China.
AD  - Global Health Institute, Health Science Center, Xi'an Jiaotong University, Xi'an, 
      712000, Shaanxi, China.
FAU - Zhao, Yan
AU  - Zhao Y
AUID- ORCID: 0000-0002-7116-8631
AD  - Department of Occupational and Environmental Health, School of Public Health, 
      Health Science Center, Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, China.
AD  - Global Health Institute, Health Science Center, Xi'an Jiaotong University, Xi'an, 
      712000, Shaanxi, China.
FAU - Wang, Qingfeng
AU  - Wang Q
AUID- ORCID: 0000-0002-1911-7570
AD  - Department of Occupational and Environmental Health, School of Public Health, 
      Health Science Center, Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, China.
AD  - Global Health Institute, Health Science Center, Xi'an Jiaotong University, Xi'an, 
      712000, Shaanxi, China.
AD  - Xi'an University of Science and Technology Hospital, Xi'an, 710054, Shaanxi, 
      China.
FAU - Liu, Haobiao
AU  - Liu H
AUID- ORCID: 0000-0003-0843-6899
AD  - Department of Occupational and Environmental Health, School of Public Health, 
      Health Science Center, Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, China.
AD  - Global Health Institute, Health Science Center, Xi'an Jiaotong University, Xi'an, 
      712000, Shaanxi, China.
FAU - You, Mei
AU  - You M
AUID- ORCID: 0000-0003-3498-2535
AD  - Department of Occupational and Environmental Health, School of Public Health, 
      Health Science Center, Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, China.
AD  - Global Health Institute, Health Science Center, Xi'an Jiaotong University, Xi'an, 
      712000, Shaanxi, China.
AD  - Shaanxi Provincial Cancer Hospital, Xi'an, 710061, Shaanxi, China.
FAU - Yuan, Qian
AU  - Yuan Q
AUID- ORCID: 0000-0002-5218-4126
AD  - Department of Occupational and Environmental Health, School of Public Health, 
      Health Science Center, Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, China.
AD  - Global Health Institute, Health Science Center, Xi'an Jiaotong University, Xi'an, 
      712000, Shaanxi, China.
FAU - Yang, Zhihao
AU  - Yang Z
AUID- ORCID: 0000-0001-6402-1458
AD  - Department of Occupational and Environmental Health, School of Public Health, 
      Health Science Center, Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, China.
AD  - Global Health Institute, Health Science Center, Xi'an Jiaotong University, Xi'an, 
      712000, Shaanxi, China.
FAU - Bian, Wenming
AU  - Bian W
AUID- ORCID: 0000-0002-9758-2179
AD  - Department of Occupational and Environmental Health, School of Public Health, 
      Health Science Center, Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, China.
AD  - Global Health Institute, Health Science Center, Xi'an Jiaotong University, Xi'an, 
      712000, Shaanxi, China.
FAU - Liu, Jiaxin
AU  - Liu J
AUID- ORCID: 0000-0001-9335-6679
AD  - Department of Occupational and Environmental Health, School of Public Health, 
      Health Science Center, Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, China.
AD  - Global Health Institute, Health Science Center, Xi'an Jiaotong University, Xi'an, 
      712000, Shaanxi, China.
FAU - Guo, Ziwei
AU  - Guo Z
AUID- ORCID: 0000-0002-8371-840X
AD  - Department of Occupational and Environmental Health, School of Public Health, 
      Health Science Center, Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, China.
AD  - Global Health Institute, Health Science Center, Xi'an Jiaotong University, Xi'an, 
      712000, Shaanxi, China.
FAU - Han, Jing
AU  - Han J
AUID- ORCID: 0000-0003-1460-0114
AD  - Department of Occupational and Environmental Health, School of Public Health, 
      Health Science Center, Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, China. 
      bbbishop@126.com.
AD  - Global Health Institute, Health Science Center, Xi'an Jiaotong University, Xi'an, 
      712000, Shaanxi, China. bbbishop@126.com.
AD  - Key Laboratory of Environment and Genes Related to Diseases, School of Public 
      Health, Health Science Center, Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, 
      China. bbbishop@126.com.
LA  - eng
GR  - 81872567/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20221025
PL  - United States
TA  - Biol Trace Elem Res
JT  - Biological trace element research
JID - 7911509
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - H6241UJ22B (Selenium)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 2.7.1.1 (mTOR protein, rat)
SB  - IM
MH  - Rats
MH  - Animals
MH  - *Proto-Oncogene Proteins c-akt/metabolism
MH  - *Selenium/pharmacology
MH  - Signal Transduction
MH  - TOR Serine-Threonine Kinases/metabolism
MH  - Liver Cirrhosis
MH  - Mammals/metabolism
OTO - NOTNLM
OT  - Akt/mTOR signaling pathway
OT  - Liver fibrosis
OT  - Micro-nutrient element
OT  - Mitochondria
OT  - Selenium deficiency
EDAT- 2022/10/26 06:00
MHDA- 2023/06/21 06:42
CRDT- 2022/10/25 11:22
PHST- 2022/08/04 00:00 [received]
PHST- 2022/10/14 00:00 [accepted]
PHST- 2023/06/21 06:42 [medline]
PHST- 2022/10/26 06:00 [pubmed]
PHST- 2022/10/25 11:22 [entrez]
AID - 10.1007/s12011-022-03453-7 [pii]
AID - 10.1007/s12011-022-03453-7 [doi]
PST - ppublish
SO  - Biol Trace Elem Res. 2023 Aug;201(8):3825-3833. doi: 10.1007/s12011-022-03453-7. 
      Epub 2022 Oct 25.