PMID- 36288301
OWN - NLM
STAT- MEDLINE
DCOM- 20221028
LR  - 20221108
IS  - 2375-2548 (Electronic)
IS  - 2375-2548 (Linking)
VI  - 8
IP  - 43
DP  - 2022 Oct 28
TI  - De novo selected hACE2 mimics that integrate hotspot peptides with aptameric 
      scaffolds for binding tolerance of SARS-CoV-2 variants.
PG  - eabq6207
LID - 10.1126/sciadv.abq6207 [doi]
LID - eabq6207
AB  - The frequent occurrence of viral variants is a critical problem in developing 
      antiviral prophylaxis and therapy; along with stronger recognition of host cell 
      receptors, the variants evade the immune system-based vaccines and neutralizing 
      agents more easily. In this work, we focus on enhanced receptor binding of viral 
      variants and demonstrate generation of receptor-mimicking synthetic reagents, 
      capable of strongly interacting with viruses and their variants. The hotspot 
      interaction of viruses with receptor-derived short peptides is maximized by 
      aptamer-like scaffolds, the compact and stable architectures of which can be in 
      vitro selected from a myriad of the hotspot peptide-coupled random nucleic acids. 
      We successfully created the human angiotensin-converting enzyme 2 (hACE2) 
      receptor-mimicking hybrid ligand that recruits the hACE2-derived receptor binding 
      domain-interacting peptide to directly interact with a binding hotspot of severe 
      acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Experiencing affinity 
      boosting by ~500% to Omicron, the de novo selected hACE2 mimic exhibited a great 
      binding tolerance to all SARS-CoV-2 variants of concern.
FAU - Lee, Minjong
AU  - Lee M
AUID- ORCID: 0000-0002-1729-9876
AD  - Department of Materials Science and Engineering, Pohang University of Science and 
      Technology (POSTECH), Pohang 37673, South Korea.
FAU - Kang, Byunghwa
AU  - Kang B
AD  - Department of Materials Science and Engineering, Pohang University of Science and 
      Technology (POSTECH), Pohang 37673, South Korea.
FAU - Lee, Juhwa
AU  - Lee J
AUID- ORCID: 0000-0002-0982-743X
AD  - Department of Chemistry, Pohang University of Science and Technology (POSTECH), 
      Pohang 37673, South Korea.
FAU - Lee, Jisun
AU  - Lee J
AD  - Department of Biomedical Sciences, Graduate School, Korea University, Seoul 
      02841, South Korea.
AD  - BK21 Graduate Program, Department of Biomedical Sciences, Korea University 
      College of Medicine, Seoul 02841, South Korea.
FAU - Jung, Sang Taek
AU  - Jung ST
AUID- ORCID: 0000-0001-7217-979X
AD  - Department of Biomedical Sciences, Graduate School, Korea University, Seoul 
      02841, South Korea.
AD  - BK21 Graduate Program, Department of Biomedical Sciences, Korea University 
      College of Medicine, Seoul 02841, South Korea.
FAU - Son, Chang Yun
AU  - Son CY
AUID- ORCID: 0000-0002-0784-6565
AD  - Department of Chemistry, Pohang University of Science and Technology (POSTECH), 
      Pohang 37673, South Korea.
AD  - Institute for Convergence Research and Education in Advanced Technology 
      (I-CREATE), Yonsei University, Incheon 21983, South Korea.
FAU - Oh, Seung Soo
AU  - Oh SS
AUID- ORCID: 0000-0001-8045-080X
AD  - Department of Materials Science and Engineering, Pohang University of Science and 
      Technology (POSTECH), Pohang 37673, South Korea.
AD  - Institute for Convergence Research and Education in Advanced Technology 
      (I-CREATE), Yonsei University, Incheon 21983, South Korea.
LA  - eng
PT  - Journal Article
DEP - 20221026
PL  - United States
TA  - Sci Adv
JT  - Science advances
JID - 101653440
RN  - EC 3.4.17.23 (Angiotensin-Converting Enzyme 2)
RN  - 0 (Spike Glycoprotein, Coronavirus)
RN  - 0 (Ligands)
RN  - 0 (Receptors, Virus)
RN  - EC 3.4.15.1 (Peptidyl-Dipeptidase A)
RN  - 0 (Peptides)
RN  - 0 (Antiviral Agents)
RN  - 0 (Nucleic Acids)
RN  - SARS-CoV-2 variants
SB  - IM
MH  - Humans
MH  - Angiotensin-Converting Enzyme 2/genetics
MH  - SARS-CoV-2/genetics
MH  - Spike Glycoprotein, Coronavirus/genetics/metabolism
MH  - Ligands
MH  - Receptors, Virus/metabolism
MH  - Peptidyl-Dipeptidase A/metabolism
MH  - *COVID-19
MH  - Protein Binding
MH  - Peptides/metabolism
MH  - Antiviral Agents
MH  - *Nucleic Acids
PMC - PMC9604513
EDAT- 2022/10/27 06:00
MHDA- 2022/10/29 06:00
PMCR- 2022/10/26
CRDT- 2022/10/26 14:04
PHST- 2022/10/26 14:04 [entrez]
PHST- 2022/10/27 06:00 [pubmed]
PHST- 2022/10/29 06:00 [medline]
PHST- 2022/10/26 00:00 [pmc-release]
AID - abq6207 [pii]
AID - 10.1126/sciadv.abq6207 [doi]
PST - ppublish
SO  - Sci Adv. 2022 Oct 28;8(43):eabq6207. doi: 10.1126/sciadv.abq6207. Epub 2022 Oct 
      26.