PMID- 36289619
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221030
IS  - 2227-9059 (Print)
IS  - 2227-9059 (Electronic)
IS  - 2227-9059 (Linking)
VI  - 10
IP  - 10
DP  - 2022 Sep 21
TI  - Amniotic Fluid-Derived Mesenchymal Stem/Stromal Cell-Derived Secretome and 
      Exosomes Improve Inflammation in Human Intestinal Subepithelial Myofibroblasts.
LID - 10.3390/biomedicines10102357 [doi]
LID - 2357
AB  - Inflammatory Bowel Diseases (IBDs) are characterized by chronic relapsing 
      inflammation of the gastrointestinal tract. The mesenchymal stem/stromal 
      cell-derived secretome and secreted extracellular vesicles may offer novel 
      therapeutic opportunities in patients with IBD. Thus, exosomes may be utilized as 
      a novel cell-free approach for IBD therapy. The aim of our study was to examine 
      the possible anti-inflammatory effects of secretome/exosomes on an IBD-relevant, 
      in vitro model of LPS-induced inflammation in human intestinal SubEpithelial 
      MyoFibroblasts (SEMFs). The tested CM (Conditioned Media)/exosomes derived from a 
      specific population of second-trimester amniotic fluid mesenchymal stem/stromal 
      cells, the spindle-shaped amniotic fluid MSCs (SS-AF-MSCs), and specifically, 
      their secreted exosomes could be utilized as a novel cell-free approach for IBD 
      therapy. Therefore, we studied the effect of SS-AF-MSCs CM and exosomes on 
      LPS-induced inflammation in SEMF cells. SS-AF-MSCs CM and exosomes were 
      collected, concentrated, and then delivered into the cell cultures. 
      Administration of both secretome and exosomes derived from SS-AF-MSCs reduced the 
      severity of LPS-induced inflammation. Specifically, IL-1beta, IL-6, TNF-alpha, and TLR-4 
      mRNA expression was decreased, while the anti-inflammatory IL-10 was elevated. 
      Our results were also verified at the protein level, as secretion of IL-1beta was 
      significantly reduced. Overall, our results highlight a cell-free and 
      anti-inflammatory therapeutic agent for potential use in IBD therapy.
FAU - Katifelis, Hector
AU  - Katifelis H
AUID- ORCID: 0000-0001-5741-4288
AD  - Department of Basic Medical Sciences, Laboratory of Biology, Medical School, 
      National and Kapodistrian University of Athens, 11527 Athens, Greece.
FAU - Filidou, Eirini
AU  - Filidou E
AD  - Laboratory of Pharmacology, Faculty of Medicine, Democritus University of Thrace, 
      68100 Alexandroupolis, Greece.
FAU - Psaraki, Adriana
AU  - Psaraki A
AD  - Department of Basic Medical Sciences, Laboratory of Biology, Medical School, 
      National and Kapodistrian University of Athens, 11527 Athens, Greece.
FAU - Yakoub, Farinta
AU  - Yakoub F
AD  - Department of Basic Medical Sciences, Laboratory of Biology, Medical School, 
      National and Kapodistrian University of Athens, 11527 Athens, Greece.
FAU - Roubelakis, Maria G
AU  - Roubelakis MG
AD  - Department of Basic Medical Sciences, Laboratory of Biology, Medical School, 
      National and Kapodistrian University of Athens, 11527 Athens, Greece.
AD  - Centre of Basic Research, Biomedical Research Foundation of the Academy of 
      Athens, 11527 Athens, Greece.
FAU - Tarapatzi, Gesthimani
AU  - Tarapatzi G
AD  - Laboratory of Pharmacology, Faculty of Medicine, Democritus University of Thrace, 
      68100 Alexandroupolis, Greece.
FAU - Vradelis, Stergios
AU  - Vradelis S
AD  - Second Department of Internal Medicine, Faculty of Medicine, Democritus 
      University of Thrace, 68100 Alexandroupolis, Greece.
FAU - Bamias, Giorgos
AU  - Bamias G
AD  - GI Unit, Sotiria Hospital, National and Kapodistrian University of Athens, 11527 
      Athens, Greece.
FAU - Kolios, George
AU  - Kolios G
AUID- ORCID: 0000-0002-2066-4782
AD  - Laboratory of Pharmacology, Faculty of Medicine, Democritus University of Thrace, 
      68100 Alexandroupolis, Greece.
FAU - Gazouli, Maria
AU  - Gazouli M
AUID- ORCID: 0000-0002-3295-6811
AD  - Department of Basic Medical Sciences, Laboratory of Biology, Medical School, 
      National and Kapodistrian University of Athens, 11527 Athens, Greece.
AD  - 2nd Department of Radiology, Medical School, National and Kapodistrian University 
      of Athens, 12462 Athens, Greece.
AD  - Department of Sciences, Hellenic Open University, 26335 Patra, Greece.
LA  - eng
PT  - Journal Article
DEP - 20220921
PL  - Switzerland
TA  - Biomedicines
JT  - Biomedicines
JID - 101691304
PMC - PMC9598363
OTO - NOTNLM
OT  - CM
OT  - IL-1beta
OT  - MSCs
OT  - TLR-4
OT  - exosomes
OT  - inflammatory bowel disease
OT  - secretome
COIS- The authors declare no conflict of interest.
EDAT- 2022/10/28 06:00
MHDA- 2022/10/28 06:01
PMCR- 2022/09/21
CRDT- 2022/10/27 01:01
PHST- 2022/08/08 00:00 [received]
PHST- 2022/09/15 00:00 [revised]
PHST- 2022/09/19 00:00 [accepted]
PHST- 2022/10/27 01:01 [entrez]
PHST- 2022/10/28 06:00 [pubmed]
PHST- 2022/10/28 06:01 [medline]
PHST- 2022/09/21 00:00 [pmc-release]
AID - biomedicines10102357 [pii]
AID - biomedicines-10-02357 [pii]
AID - 10.3390/biomedicines10102357 [doi]
PST - epublish
SO  - Biomedicines. 2022 Sep 21;10(10):2357. doi: 10.3390/biomedicines10102357.