PMID- 36290084
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230308
IS  - 2079-6382 (Print)
IS  - 2079-6382 (Electronic)
IS  - 2079-6382 (Linking)
VI  - 11
IP  - 10
DP  - 2022 Oct 17
TI  - Highly Sensitive Determination of Antibiotic Residues in Aquatic Products by 
      High-Performance Liquid Chromatography-Tandem Mass Spectrometry.
LID - 10.3390/antibiotics11101427 [doi]
LID - 1427
AB  - Antibiotic drug residues are crucial to ensure food safety and minimize risk to 
      human health. Herein, a sensitive high-performance liquid chromatography-tandem 
      mass spectrometry (HPLC-MS/MS) method was developed and validated for the 
      determination of antibiotic residues (mainly amphenicols) consisting of 
      chloramphenicol (CAP), thiamphenicol (TAP), florfenicol (FF), and florfenicol 
      amine (FFA) in aquatic products. Amphenicols were well separated on a Kinetex F5 
      (100 mm x 3.0 mm, 2.6 microm) chromatographic column with the mobile phases of 1 mM 
      ammonium acetate aqueous solution and methanol solution and measured after 
      positive and negative electrospray ionizations using four internal standards. To 
      our knowledge, it was the first time to report the good performance of F5 column 
      and four internal standards for the determination of amphenicols. The established 
      method featured a good linear relationship between chromatographic peak area 
      ratios and the concentrations of amphenicols (R2 > 0.992), a wide and low 
      detection matrix-based range of 0.01-5 mug/L, a low detection limit of 0.01 mug/kg, 
      etc. The spiked assays evidenced the accuracy and reliability of the developed 
      method with the recoveries between 84.0 and 105%, the intraday relative standard 
      deviations (RSDs) over the range of 0.769-13.7%, and the interday RSDs over the 
      range of 0.582-13.3%. Finally, the proposed method was applied to investigate 
      amphenicol residues in various aquatic products, including fish, shrimp, crab, 
      shellfish, and other aquatic species.
FAU - Ye, Hongli
AU  - Ye H
AUID- ORCID: 0000-0001-9253-2300
AD  - Laboratory of Aquatic Product Quality, Safety and Processing, East China Sea 
      Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 
      200090, China.
AD  - Key Laboratory of Control of Safety and Quality for Aquatic Product, Ministry of 
      Agriculture and Rural Affairs, Beijing 100141, China.
FAU - Li, Siman
AU  - Li S
AUID- ORCID: 0000-0003-2616-986X
AD  - Laboratory of Aquatic Product Quality, Safety and Processing, East China Sea 
      Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 
      200090, China.
FAU - Xi, Yinfeng
AU  - Xi Y
AD  - Laboratory of Aquatic Product Quality, Safety and Processing, East China Sea 
      Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 
      200090, China.
AD  - Key Laboratory of Control of Safety and Quality for Aquatic Product, Ministry of 
      Agriculture and Rural Affairs, Beijing 100141, China.
FAU - Shi, Yongfu
AU  - Shi Y
AD  - Laboratory of Aquatic Product Quality, Safety and Processing, East China Sea 
      Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 
      200090, China.
FAU - Shang, Xiaorui
AU  - Shang X
AD  - Laboratory of Aquatic Product Quality, Safety and Processing, East China Sea 
      Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 
      200090, China.
AD  - College of Food Sciences and Technology, Shanghai Ocean University, Shanghai 
      201306, China.
FAU - Huang, Dongmei
AU  - Huang D
AD  - Laboratory of Aquatic Product Quality, Safety and Processing, East China Sea 
      Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 
      200090, China.
LA  - eng
GR  - no. 2019T12/the Central Public-Interest Scientific Institution Basal Research 
      Fund (East China Sea Fisheries Research Institute)/
GR  - no. F01108/Shanghai Science and Technology Agricultural Development Project/
PT  - Journal Article
DEP - 20221017
PL  - Switzerland
TA  - Antibiotics (Basel)
JT  - Antibiotics (Basel, Switzerland)
JID - 101637404
PMC - PMC9598229
OTO - NOTNLM
OT  - HPLC-MS/MS
OT  - amphenicols
OT  - antibiotics
OT  - aquatic products
OT  - detection
OT  - sensitivity
COIS- The authors declare no conflict of interest.
EDAT- 2022/10/28 06:00
MHDA- 2022/10/28 06:01
PMCR- 2022/10/17
CRDT- 2022/10/27 01:04
PHST- 2022/08/15 00:00 [received]
PHST- 2022/10/07 00:00 [revised]
PHST- 2022/10/14 00:00 [accepted]
PHST- 2022/10/27 01:04 [entrez]
PHST- 2022/10/28 06:00 [pubmed]
PHST- 2022/10/28 06:01 [medline]
PHST- 2022/10/17 00:00 [pmc-release]
AID - antibiotics11101427 [pii]
AID - antibiotics-11-01427 [pii]
AID - 10.3390/antibiotics11101427 [doi]
PST - epublish
SO  - Antibiotics (Basel). 2022 Oct 17;11(10):1427. doi: 10.3390/antibiotics11101427.