PMID- 36290579
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221030
IS  - 2076-3921 (Print)
IS  - 2076-3921 (Electronic)
IS  - 2076-3921 (Linking)
VI  - 11
IP  - 10
DP  - 2022 Sep 20
TI  - Expression Analysis of a Novel Oxidoreductase Glutaredoxin 2 in Black Tiger 
      Shrimp, Penaeus monodon.
LID - 10.3390/antiox11101857 [doi]
LID - 1857
AB  - Glutaredoxin (Grx) is a glutathione-dependent oxidoreductase that is an important 
      component of the redox system in organisms. However, there is a serious lack of 
      sequence information and functional validation related to Grx in crustaceans. In 
      this study, a novel Grx was identified in Penaeus monodon (PmGrx2). The 
      full-length cDNA of PmGrx2 is 998 bp, with an open reading frame (ORF) of 441 bp, 
      encoding 119 amino acids. Sequence alignment showed that PmGrx2 had the highest 
      identity with Grx2 of Penaeus vannamei at 96.64% and clustered with Grx2 of other 
      crustaceans. Quantitative real-time PCR (qRT-PCR) analysis showed that PmGrx2 was 
      expressed in all examined tissues, with higher expression levels in the stomach 
      and testis. PmGrx2 was continuously expressed during development and had the 
      highest expression level in the zygote stage. Both ammonia-N stress and bacterial 
      infection could differentially induce the expression of PmGrx2 in hepatopancreas 
      and gills. When PmGrx2 was inhibited, the expression of antioxidant enzymes was 
      suppressed, the degree of apoptosis increased, and the GSH content decreased with 
      the prolongation of ammonia-N stress. Inhibition of PmGrx2 resulted in shrimp 
      being exposed to a greater risk of oxidative damage. In addition, an SNP locus 
      was screened on the exons of PmGrx2 that was significantly associated with an 
      ammonia-N-stress-tolerance trait. This study suggests that PmGrx2 is involved in 
      redox regulation and plays an important role in shrimps' resistance to marine 
      environmental stresses.
FAU - Fan, Rui
AU  - Fan R
AD  - Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, 
      Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research 
      Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China.
AD  - College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 
      201306, China.
FAU - Li, Yundong
AU  - Li Y
AD  - Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, 
      Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research 
      Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China.
FAU - Yang, Qibin
AU  - Yang Q
AD  - Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, 
      Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research 
      Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China.
AD  - Tropical Fishery Research and Development Center, South China Sea Fisheries 
      Research Institute, Chinese Academy of Fishery Sciences, Sanya 572018, China.
FAU - Jiang, Song
AU  - Jiang S
AD  - Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, 
      Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research 
      Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China.
FAU - Huang, Jianhua
AU  - Huang J
AD  - Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, 
      Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research 
      Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China.
FAU - Yang, Lishi
AU  - Yang L
AD  - Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, 
      Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research 
      Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China.
FAU - Chen, Xu
AU  - Chen X
AD  - Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, 
      Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research 
      Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China.
AD  - Tropical Fishery Research and Development Center, South China Sea Fisheries 
      Research Institute, Chinese Academy of Fishery Sciences, Sanya 572018, China.
FAU - Zhou, Falin
AU  - Zhou F
AD  - Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, 
      Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research 
      Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China.
AD  - Tropical Fishery Research and Development Center, South China Sea Fisheries 
      Research Institute, Chinese Academy of Fishery Sciences, Sanya 572018, China.
FAU - Jiang, Shigui
AU  - Jiang S
AD  - Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, 
      Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research 
      Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China.
LA  - eng
PT  - Journal Article
DEP - 20220920
PL  - Switzerland
TA  - Antioxidants (Basel)
JT  - Antioxidants (Basel, Switzerland)
JID - 101668981
PMC - PMC9598912
OTO - NOTNLM
OT  - Penaeus monodon
OT  - SNPs
OT  - glutaredoxin
OT  - oxidative stress
COIS- The authors declare no conflict of interest.
EDAT- 2022/10/28 06:00
MHDA- 2022/10/28 06:01
PMCR- 2022/09/20
CRDT- 2022/10/27 01:07
PHST- 2022/08/23 00:00 [received]
PHST- 2022/09/15 00:00 [revised]
PHST- 2022/09/19 00:00 [accepted]
PHST- 2022/10/27 01:07 [entrez]
PHST- 2022/10/28 06:00 [pubmed]
PHST- 2022/10/28 06:01 [medline]
PHST- 2022/09/20 00:00 [pmc-release]
AID - antiox11101857 [pii]
AID - antioxidants-11-01857 [pii]
AID - 10.3390/antiox11101857 [doi]
PST - epublish
SO  - Antioxidants (Basel). 2022 Sep 20;11(10):1857. doi: 10.3390/antiox11101857.