PMID- 36291023
OWN - NLM
STAT- MEDLINE
DCOM- 20221028
LR  - 20221030
IS  - 2079-6374 (Electronic)
IS  - 2079-6374 (Linking)
VI  - 12
IP  - 10
DP  - 2022 Oct 17
TI  - Colorimetric Detection of Urease-Producing Microbes Using an Ammonia-Responsive 
      Flexible Film Sensor.
LID - 10.3390/bios12100886 [doi]
LID - 886
AB  - Urease-producing (ureolytic) microbes have given rise to environmental and public 
      health concerns because they are thought to contribute to emissions of ammonia 
      and to be a virulence factor for infections. Therefore, it is highly important to 
      have the ability to detect such microbes. In this study, a poly(dimethylsiloxane) 
      (PDMS)-based colorimetric film sensor was employed for the detection of 
      urease-producing microbes. The sensor was able to detect the enzyme activity of 
      commercially available urease, as the color and absorbance spectrum of the sensor 
      was observed to change upon being exposed to the reaction catalyzed by urease. 
      The ratio of the absorbance of the sensor at 640 nm to that at 460 nm 
      (A(640)/A(460)) was linearly proportional to the amount of urease present. The 
      performance of the sensor was validated by the results of a sensitivity and 
      selectivity analysis towards thirteen different bacterial strains. Based on the 
      development of blue color of the sensor, the tested bacteria were classified as 
      strongly positive, moderately positive, weakly positive, or negative urease 
      producers. The response of the sensor to ureolytic bacteria was verified using 
      the urease inhibitor phenyl phosphorodiamidate (PPDA). Additionally, the sensor 
      achieved the selective detection of ureolytic bacteria even in the presence of 
      non-ureolytic bacteria. In addition, a used sensor could be reverted to its 
      original state by being subjected to simple aeration, and in this way the same 
      sensor could be used at least five times for the detection of bacterial urease 
      activity.
FAU - Chang, Yunsoo
AU  - Chang Y
AUID- ORCID: 0000-0002-4784-2024
AD  - Department of Microbiology, Pusan National University, Busan 46241, Korea.
FAU - Park, Tae-Eon
AU  - Park TE
AUID- ORCID: 0000-0003-0192-2771
AD  - Center for Spintronics, Korea Institute of Science and Technology, Seoul 02792, 
      Korea.
FAU - Lee, Seung-Woo
AU  - Lee SW
AUID- ORCID: 0000-0003-4627-4712
AD  - Department of Fine Chemistry, Seoul National University of Science and 
      Technology, Seoul 01811, Korea.
AD  - Center for Functional Biomaterials, Seoul National University of Science and 
      Technology, Seoul 01811, Korea.
FAU - Lee, Eun-Hee
AU  - Lee EH
AUID- ORCID: 0000-0002-4388-8092
AD  - Department of Microbiology, Pusan National University, Busan 46241, Korea.
LA  - eng
GR  - Not applicable/Seoul National University of Science and Technology/
PT  - Journal Article
DEP - 20221017
PL  - Switzerland
TA  - Biosensors (Basel)
JT  - Biosensors
JID - 101609191
RN  - EC 3.5.1.5 (Urease)
RN  - 7664-41-7 (Ammonia)
RN  - 0 (Virulence Factors)
SB  - IM
MH  - *Urease
MH  - *Ammonia
MH  - Colorimetry
MH  - Bacteria
MH  - Virulence Factors
PMC - PMC9599750
OTO - NOTNLM
OT  - colorimetry
OT  - film
OT  - pH-sensitive
OT  - reusable sensor
OT  - urease
OT  - ureolytic microorganism
COIS- The authors declare no conflict of interest.
EDAT- 2022/10/28 06:00
MHDA- 2022/10/29 06:00
PMCR- 2022/10/17
CRDT- 2022/10/27 01:09
PHST- 2022/08/29 00:00 [received]
PHST- 2022/10/08 00:00 [revised]
PHST- 2022/10/11 00:00 [accepted]
PHST- 2022/10/27 01:09 [entrez]
PHST- 2022/10/28 06:00 [pubmed]
PHST- 2022/10/29 06:00 [medline]
PHST- 2022/10/17 00:00 [pmc-release]
AID - bios12100886 [pii]
AID - biosensors-12-00886 [pii]
AID - 10.3390/bios12100886 [doi]
PST - epublish
SO  - Biosensors (Basel). 2022 Oct 17;12(10):886. doi: 10.3390/bios12100886.