PMID- 36293482
OWN - NLM
STAT- MEDLINE
DCOM- 20221028
LR  - 20221030
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 23
IP  - 20
DP  - 2022 Oct 20
TI  - Mechanical Stimulation Alters Chronic Ethanol-Induced Changes to VTA GABA 
      Neurons, NAc DA Release and Measures of Withdrawal.
LID - 10.3390/ijms232012630 [doi]
LID - 12630
AB  - Therapeutic activation of mechanoreceptors (MStim) in osteopathy, chiropractic 
      and acupuncture has been in use for hundreds of years with a myriad of positive 
      outcomes. It has been previously shown to modulate the firing rate of neurons in 
      the ventral tegmental area (VTA) and dopamine (DA) release in the nucleus 
      accumbens (NAc), an area of interest in alcohol-use disorder (AUD). In this 
      study, we examined the effects of MStim on VTA GABA neuron firing rate, DA 
      release in the NAc, and behavior during withdrawal from chronic EtOH exposure in 
      a rat model. We demonstrate that concurrent administration of MStim and EtOH 
      significantly reduced adaptations in VTA GABA neurons and DA release in response 
      to a reinstatement dose of EtOH (2.5 g/kg). Behavioral indices of EtOH withdrawal 
      (rearing, open-field crosses, tail stiffness, gait, and anxiety) were 
      substantively ameliorated with concurrent application of MStim. Additionally, 
      MStim significantly increased the overall frequency of ultrasonic vocalizations, 
      suggesting an increased positive affective state.
FAU - Bills, Kyle B
AU  - Bills KB
AD  - Department of Biomedical Sciences, Noorda College of Osteopathic Medicine, Provo, 
      UT 84606, USA.
AD  - Department of Psychology/Neuroscience, Brigham Young University, Provo, UT 84602, 
      USA.
FAU - Otteson, Dallin Z
AU  - Otteson DZ
AD  - Department of Psychology/Neuroscience, Brigham Young University, Provo, UT 84602, 
      USA.
FAU - Jones, Gavin C
AU  - Jones GC
AUID- ORCID: 0000-0002-6294-5355
AD  - Department of Psychology/Neuroscience, Brigham Young University, Provo, UT 84602, 
      USA.
FAU - Brundage, James N
AU  - Brundage JN
AD  - Department of Psychology/Neuroscience, Brigham Young University, Provo, UT 84602, 
      USA.
FAU - Baldwin, Emily K
AU  - Baldwin EK
AD  - Department of Psychology/Neuroscience, Brigham Young University, Provo, UT 84602, 
      USA.
FAU - Small, Christina A
AU  - Small CA
AD  - Department of Psychology/Neuroscience, Brigham Young University, Provo, UT 84602, 
      USA.
FAU - Kim, Hee Young
AU  - Kim HY
AUID- ORCID: 0000-0002-2495-9115
AD  - Department of Physiology, Yonsei University College of Medicine, Seoul 03722, 
      Korea.
FAU - Yorgason, Jordan T
AU  - Yorgason JT
AUID- ORCID: 0000-0002-5687-0676
AD  - Department of Cellular Biology and Physiology, Brigham Young University, Provo, 
      UT 84602, USA.
FAU - Blotter, Jonathan D
AU  - Blotter JD
AD  - Department of Engineering, Brigham Young University, Provo, UT 84602, USA.
FAU - Steffensen, Scott C
AU  - Steffensen SC
AUID- ORCID: 0000-0001-6463-8861
AD  - Department of Psychology/Neuroscience, Brigham Young University, Provo, UT 84602, 
      USA.
LA  - eng
GR  - F32AT009945/NH/NIH HHS/United States
GR  - 20146802/American Osteopathic Association/
PT  - Journal Article
DEP - 20221020
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - VTD58H1Z2X (Dopamine)
RN  - 3K9958V90M (Ethanol)
SB  - IM
MH  - Rats
MH  - Animals
MH  - *Ventral Tegmental Area
MH  - *Dopamine/pharmacology
MH  - GABAergic Neurons
MH  - Ethanol/pharmacology
MH  - Nucleus Accumbens
PMC - PMC9604215
OTO - NOTNLM
OT  - alcohol
OT  - dopamine
OT  - mechanoreceptors
OT  - physical medicine
COIS- The authors (K.B.B., S.C.S., D.Z.O., G.C.J., J.N.B., E.K.B., C.A.S., J.T.Y., 
      J.D.B. and H.Y.K) declare no conflict of interest.
EDAT- 2022/10/28 06:00
MHDA- 2022/10/29 06:00
PMCR- 2022/10/20
CRDT- 2022/10/27 01:24
PHST- 2022/09/23 00:00 [received]
PHST- 2022/10/11 00:00 [revised]
PHST- 2022/10/14 00:00 [accepted]
PHST- 2022/10/27 01:24 [entrez]
PHST- 2022/10/28 06:00 [pubmed]
PHST- 2022/10/29 06:00 [medline]
PHST- 2022/10/20 00:00 [pmc-release]
AID - ijms232012630 [pii]
AID - ijms-23-12630 [pii]
AID - 10.3390/ijms232012630 [doi]
PST - epublish
SO  - Int J Mol Sci. 2022 Oct 20;23(20):12630. doi: 10.3390/ijms232012630.