PMID- 36293557
OWN - NLM
STAT- MEDLINE
DCOM- 20221028
LR  - 20221030
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 23
IP  - 20
DP  - 2022 Oct 21
TI  - Tissue Sheet Engineered Using Human Umbilical Cord-Derived Mesenchymal Stem Cells 
      Improves Diabetic Wound Healing.
LID - 10.3390/ijms232012697 [doi]
LID - 12697
AB  - Diabetic foot ulceration is a common chronic diabetic complication. Human 
      umbilical cord-derived mesenchymal stem cells (hUC-MSCs) have been widely used in 
      regenerative medicine owing to their multipotency and easy availability. We 
      developed poly(lactic-co-glycolic acid) (PLGA)-based scaffold to create hUC-MSC 
      tissue sheets. In vitro immunostaining showed that hUC-MSC tissue sheets formed 
      thick and solid tissue sheets with an abundance of extracellular matrix (ECM). 
      Diabetic wounds in mice treated with or without either the hUC-MSC tissue sheet, 
      hUC-MSC injection, or fiber only revealed that hUC-MSC tissue sheet 
      transplantation promoted diabetic wound healing with improved 
      re-epithelialization, collagen deposition, blood vessel formation and maturation, 
      and alleviated inflammation compared to that observed in other groups. Taken 
      collectively, our findings suggest that hUC-MSCs cultured on PLGA scaffolds 
      improve diabetic wound healing, collagen deposition, and angiogenesis, and 
      provide a novel and effective method for cell transplantation, and a promising 
      alternative for diabetic skin wound treatment.
FAU - Zhang, Jingbo
AU  - Zhang J
AUID- ORCID: 0000-0002-9426-209X
AD  - Department of Cardiovascular Surgery, Osaka University Graduate School of 
      Medicine, 2-2 Yamada-oka, Suita 565-0871, Osaka, Japan.
FAU - Qu, Xiang
AU  - Qu X
AD  - Department of Cardiovascular Surgery, Osaka University Graduate School of 
      Medicine, 2-2 Yamada-oka, Suita 565-0871, Osaka, Japan.
FAU - Li, Junjun
AU  - Li J
AUID- ORCID: 0000-0002-7473-4918
AD  - Department of Cardiovascular Surgery, Osaka University Graduate School of 
      Medicine, 2-2 Yamada-oka, Suita 565-0871, Osaka, Japan.
FAU - Harada, Akima
AU  - Harada A
AD  - Department of Cardiovascular Surgery, Osaka University Graduate School of 
      Medicine, 2-2 Yamada-oka, Suita 565-0871, Osaka, Japan.
FAU - Hua, Ying
AU  - Hua Y
AD  - Department of Cardiovascular Surgery, Osaka University Graduate School of 
      Medicine, 2-2 Yamada-oka, Suita 565-0871, Osaka, Japan.
FAU - Yoshida, Noriko
AU  - Yoshida N
AD  - Department of Cardiovascular Surgery, Osaka University Graduate School of 
      Medicine, 2-2 Yamada-oka, Suita 565-0871, Osaka, Japan.
FAU - Ishida, Masako
AU  - Ishida M
AD  - Department of Cardiovascular Surgery, Osaka University Graduate School of 
      Medicine, 2-2 Yamada-oka, Suita 565-0871, Osaka, Japan.
FAU - Sawa, Yoshiki
AU  - Sawa Y
AD  - Department of Cardiovascular Surgery, Osaka University Graduate School of 
      Medicine, 2-2 Yamada-oka, Suita 565-0871, Osaka, Japan.
FAU - Liu, Li
AU  - Liu L
AD  - Department of Cardiovascular Surgery, Osaka University Graduate School of 
      Medicine, 2-2 Yamada-oka, Suita 565-0871, Osaka, Japan.
FAU - Miyagawa, Shigeru
AU  - Miyagawa S
AD  - Department of Cardiovascular Surgery, Osaka University Graduate School of 
      Medicine, 2-2 Yamada-oka, Suita 565-0871, Osaka, Japan.
LA  - eng
GR  - 22H03157/Japan Society for the Promotion of Science/
GR  - 22K12801/Japan Society for the Promotion of Science/
PT  - Journal Article
DEP - 20221021
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Humans
MH  - Mice
MH  - Animals
MH  - *Mesenchymal Stem Cell Transplantation/methods
MH  - Umbilical Cord
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - *Mesenchymal Stem Cells
MH  - Wound Healing
MH  - Collagen
MH  - *Diabetes Mellitus
PMC - PMC9604116
OTO - NOTNLM
OT  - PLGA scaffold
OT  - collagen deposition
OT  - diabetic wound
OT  - human umbilical cord-derived mesenchymal stem cells
OT  - inflammation
OT  - wound healing
COIS- Yoshiki Sawa is the chief advisor of Cell Exosomes Therapeutics Co., Ltd. The 
      authors declare that they have no competing non-financial interests.
EDAT- 2022/10/28 06:00
MHDA- 2022/10/29 06:00
PMCR- 2022/10/21
CRDT- 2022/10/27 01:25
PHST- 2022/09/29 00:00 [received]
PHST- 2022/10/17 00:00 [revised]
PHST- 2022/10/20 00:00 [accepted]
PHST- 2022/10/27 01:25 [entrez]
PHST- 2022/10/28 06:00 [pubmed]
PHST- 2022/10/29 06:00 [medline]
PHST- 2022/10/21 00:00 [pmc-release]
AID - ijms232012697 [pii]
AID - ijms-23-12697 [pii]
AID - 10.3390/ijms232012697 [doi]
PST - epublish
SO  - Int J Mol Sci. 2022 Oct 21;23(20):12697. doi: 10.3390/ijms232012697.