PMID- 36296679
OWN - NLM
STAT- MEDLINE
DCOM- 20221028
LR  - 20221030
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 27
IP  - 20
DP  - 2022 Oct 20
TI  - Structural Prediction and Characterization of Canavalia grandiflora (ConGF) 
      Lectin Complexed with MMP1: Unveiling the Antiglioma Potential of Legume Lectins.
LID - 10.3390/molecules27207089 [doi]
LID - 7089
AB  - A glioblastoma (GBM) is a highly malignant primary brain tumor with a poor 
      prognosis because of its invasiveness and high resistance to current therapies. 
      In GBMs, abnormal glycosylation patterns are associated with malignancy, which 
      allows for the use of lectins as tools for recognition and therapy. More 
      specifically, lectins can interact with glycan structures found on the malignant 
      cell surface. In this context, the present work aimed to investigate the 
      antiglioma potential of ConGF, a lectin purified from Canavalia grandiflora 
      seeds, against C6 cells. The treatment of C6 cells with ConGF impaired the 
      mitochondrial transmembrane potential, reduced cell viability, and induced 
      morphological changes. ConGF also induced massive autophagy, as evaluated by 
      acridine orange (AO) staining and LC3AB-II expression, but without prominent 
      propidium iodide (PI) labeling. The mechanism of action appears to involve the 
      carbohydrate-binding capacity of ConGF, and in silico studies suggested that the 
      lectin can interact with the glycan structures of matrix metalloproteinase 1 
      (MMP1), a prominent protein found in malignant cells, likely explaining the 
      observed effects.
FAU - Leal, Rodrigo Bainy
AU  - Leal RB
AUID- ORCID: 0000-0003-2536-6888
AD  - Departamento de Bioquimica, Universidade Federal de Santa Catarina (UFSC), 
      Florianopolis 88040-900, SC, Brazil.
FAU - Mann, Josiane
AU  - Mann J
AUID- ORCID: 0000-0001-9617-5400
AD  - Departamento de Bioquimica, Universidade Federal de Santa Catarina (UFSC), 
      Florianopolis 88040-900, SC, Brazil.
FAU - Pinto-Junior, Vanir Reis
AU  - Pinto-Junior VR
AD  - Departamento de Fisica, Universidade Federal do Ceara (UFC), Fortaleza 60440-970, 
      CE, Brazil.
AD  - Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara 
      (UFC), Fortaleza 60440-970, CE, Brazil.
FAU - Oliveira, Messias Vital
AU  - Oliveira MV
AD  - Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara 
      (UFC), Fortaleza 60440-970, CE, Brazil.
FAU - Osterne, Vinicius Jose Silva
AU  - Osterne VJS
AD  - Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara 
      (UFC), Fortaleza 60440-970, CE, Brazil.
AD  - Department of Biotechnology, Ghent University, 9000 Ghent, Belgium.
FAU - Wolin, Ingrid Alessandra Victoria
AU  - Wolin IAV
AD  - Departamento de Bioquimica, Universidade Federal de Santa Catarina (UFSC), 
      Florianopolis 88040-900, SC, Brazil.
FAU - Nascimento, Ana Paula Machado
AU  - Nascimento APM
AD  - Departamento de Bioquimica, Universidade Federal de Santa Catarina (UFSC), 
      Florianopolis 88040-900, SC, Brazil.
FAU - Welter, Priscilla Gomes
AU  - Welter PG
AUID- ORCID: 0000-0002-0278-7746
AD  - Departamento de Bioquimica, Universidade Federal de Santa Catarina (UFSC), 
      Florianopolis 88040-900, SC, Brazil.
FAU - Ferreira, Valeria Maria Sousa
AU  - Ferreira VMS
AD  - Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara 
      (UFC), Fortaleza 60440-970, CE, Brazil.
FAU - Silva, Alice Araujo
AU  - Silva AA
AD  - Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara 
      (UFC), Fortaleza 60440-970, CE, Brazil.
FAU - Seeger, Rodrigo Lopes
AU  - Seeger RL
AD  - Departamento de Bioquimica, Universidade Federal de Santa Catarina (UFSC), 
      Florianopolis 88040-900, SC, Brazil.
FAU - Nascimento, Kyria Santiago
AU  - Nascimento KS
AD  - Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara 
      (UFC), Fortaleza 60440-970, CE, Brazil.
FAU - Cavada, Benildo Sousa
AU  - Cavada BS
AUID- ORCID: 0000-0002-5791-6170
AD  - Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara 
      (UFC), Fortaleza 60440-970, CE, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20221020
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (Lectins)
RN  - EC 3.4.24.7 (Matrix Metalloproteinase 1)
RN  - 36015-30-2 (Propidium)
RN  - F30N4O6XVV (Acridine Orange)
RN  - 0 (Plant Lectins)
RN  - 0 (Carbohydrates)
SB  - IM
MH  - *Canavalia/chemistry
MH  - *Fabaceae/chemistry
MH  - Lectins/chemistry
MH  - Matrix Metalloproteinase 1
MH  - Propidium
MH  - Acridine Orange
MH  - Plant Lectins/chemistry
MH  - Seeds/chemistry
MH  - Carbohydrates/analysis
PMC - PMC9612022
OTO - NOTNLM
OT  - Canavalia grandiflora
OT  - glioblastoma
OT  - lectin
OT  - matrix metalloproteinase 1
COIS- The authors declare no conflict of interest.
EDAT- 2022/10/28 06:00
MHDA- 2022/10/29 06:00
PMCR- 2022/10/20
CRDT- 2022/10/27 01:45
PHST- 2022/09/21 00:00 [received]
PHST- 2022/10/13 00:00 [revised]
PHST- 2022/10/17 00:00 [accepted]
PHST- 2022/10/27 01:45 [entrez]
PHST- 2022/10/28 06:00 [pubmed]
PHST- 2022/10/29 06:00 [medline]
PHST- 2022/10/20 00:00 [pmc-release]
AID - molecules27207089 [pii]
AID - molecules-27-07089 [pii]
AID - 10.3390/molecules27207089 [doi]
PST - epublish
SO  - Molecules. 2022 Oct 20;27(20):7089. doi: 10.3390/molecules27207089.