PMID- 36298720
OWN - NLM
STAT- MEDLINE
DCOM- 20221028
LR  - 20221104
IS  - 1999-4915 (Electronic)
IS  - 1999-4915 (Linking)
VI  - 14
IP  - 10
DP  - 2022 Sep 30
TI  - Epidemiological Analysis of Cassava Mosaic and Brown Streak Diseases, and Bemisia 
      tabaci in the Comoros Islands.
LID - 10.3390/v14102165 [doi]
LID - 2165
AB  - A comprehensive assessment of cassava brown streak disease (CBSD) and cassava 
      mosaic disease (CMD) was carried out in Comoros where cassava yield (5.7 t/ha) is 
      significantly below the African average (8.6 t/ha) largely due to virus diseases. 
      Observations from 66 sites across the Comoros Islands of Mwali, Ngazidja, and 
      Ndzwani revealed that 83.3% of cassava fields had foliar symptoms of CBSD 
      compared with 95.5% for CMD. Molecular diagnostics confirmed the presence of both 
      cassava brown streak ipomoviruses (CBSIs) and cassava mosaic begomoviruses 
      (CMBs). Although real-time RT-PCR only detected the presence of one CBSI species 
      (Cassava brown streak virus, CBSV) the second species (Ugandan cassava brown 
      streak virus, UCBSV) was identified using next-generation high-throughput 
      sequencing. Both PCR and HTS detected the presence of East African cassava mosaic 
      virus (EACMV). African cassava mosaic virus was not detected in any of the 
      samples. Four whitefly species were identified from a sample of 131 specimens: 
      Bemisia tabaci, B. afer, Aleurodicus dispersus, and Paraleyrodes bondari. Cassava 
      B. tabaci comprised two mitotypes: SSA1-SG2 (89%) and SSA1-SG3 (11%). KASP SNP 
      genotyping categorized 82% of cassava B. tabaci as haplogroup SSA-ESA. This 
      knowledge will provide an important base for developing and deploying effective 
      management strategies for cassava viruses and their vectors.
FAU - Shirima, Rudolph Rufini
AU  - Shirima RR
AUID- ORCID: 0000-0002-2905-2678
AD  - International Institute of Tropical Agriculture (IITA-Tanzania), P.O. Box 34441, 
      Dar es Salaam 14112, Tanzania.
FAU - Wosula, Everlyne Nafula
AU  - Wosula EN
AUID- ORCID: 0000-0001-5693-0889
AD  - International Institute of Tropical Agriculture (IITA-Tanzania), P.O. Box 34441, 
      Dar es Salaam 14112, Tanzania.
FAU - Hamza, Abdou Azali
AU  - Hamza AA
AD  - Institut National de Recherche pour L'Agriculture, La Peche et L'Environnement 
      (INRAPE), Moroni BP 1406, Comoros.
FAU - Mohammed, Nobataine Ali
AU  - Mohammed NA
AD  - Institut National de Recherche pour L'Agriculture, La Peche et L'Environnement 
      (INRAPE), Moroni BP 1406, Comoros.
FAU - Mouigni, Hadji
AU  - Mouigni H
AD  - Institut National de Recherche pour L'Agriculture, La Peche et L'Environnement 
      (INRAPE), Moroni BP 1406, Comoros.
FAU - Nouhou, Salima
AU  - Nouhou S
AD  - Institut National de Recherche pour L'Agriculture, La Peche et L'Environnement 
      (INRAPE), Moroni BP 1406, Comoros.
FAU - Mchinda, Naima Mmadi
AU  - Mchinda NM
AD  - Institut National de Recherche pour L'Agriculture, La Peche et L'Environnement 
      (INRAPE), Moroni BP 1406, Comoros.
FAU - Ceasar, Gloria
AU  - Ceasar G
AD  - International Institute of Tropical Agriculture (IITA-Tanzania), P.O. Box 34441, 
      Dar es Salaam 14112, Tanzania.
FAU - Amour, Massoud
AU  - Amour M
AD  - International Institute of Tropical Agriculture (IITA-Tanzania), P.O. Box 34441, 
      Dar es Salaam 14112, Tanzania.
FAU - Njukwe, Emmanuel
AU  - Njukwe E
AD  - West and Central African Council for Agricultural Research and Development 
      (CORAF), Dakar CP 18523, Senegal.
FAU - Legg, James Peter
AU  - Legg JP
AUID- ORCID: 0000-0003-4140-3757
AD  - International Institute of Tropical Agriculture (IITA-Tanzania), P.O. Box 34441, 
      Dar es Salaam 14112, Tanzania.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220930
PL  - Switzerland
TA  - Viruses
JT  - Viruses
JID - 101509722
SB  - IM
MH  - Animals
MH  - *Manihot
MH  - Comoros
MH  - Plant Diseases
MH  - *Hemiptera
PMC - PMC9608219
OTO - NOTNLM
OT  - Bemisia tabaci
OT  - CBSD
OT  - CBSIs
OT  - CMBs
OT  - CMD
OT  - HTS
OT  - surveillance
COIS- The authors declare no conflict of interest. The funders had no role in the 
      design of the study; in the collection, analyses, or interpretation of data; in 
      the writing of the manuscript; or in the decision to publish the results.
EDAT- 2022/10/28 06:00
MHDA- 2022/10/29 06:00
PMCR- 2022/09/30
CRDT- 2022/10/27 01:58
PHST- 2022/08/02 00:00 [received]
PHST- 2022/09/15 00:00 [revised]
PHST- 2022/09/28 00:00 [accepted]
PHST- 2022/10/27 01:58 [entrez]
PHST- 2022/10/28 06:00 [pubmed]
PHST- 2022/10/29 06:00 [medline]
PHST- 2022/09/30 00:00 [pmc-release]
AID - v14102165 [pii]
AID - viruses-14-02165 [pii]
AID - 10.3390/v14102165 [doi]
PST - epublish
SO  - Viruses. 2022 Sep 30;14(10):2165. doi: 10.3390/v14102165.