PMID- 36299332
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221028
IS  - 2213-8862 (Electronic)
IS  - 1991-7902 (Print)
IS  - 1991-7902 (Linking)
VI  - 17
IP  - 4
DP  - 2022 Oct
TI  - The different irradiation parameters of carbon dioxide laser effects on 
      periodontal ligament cells.
PG  - 1751-1761
LID - 10.1016/j.jds.2022.05.016 [doi]
AB  - BACKGROUND: /purpose: Photobiostimulation (PBS) can affect cellular functions. 
      The objective of the present study was to evaluate the cellular changes in 
      periodontal ligament (PDL) cells that received different carbon dioxide (CO(2)) 
      laser irradiation parameters under negative pressure culture. MATERIALS AND 
      METHODS: The negative pressure-cultured PDL cells on normal medium and 
      differentiation medium were subjected to continuous irradiation with a CO(2) 
      laser at an energy density of 5 J/cm(2) or 10 J/cm(2). The irradiated PDL cells 
      were harvested at Days 1, 5 and 7, and their viability was analyzed by the Presto 
      Blue assay and the biologic markers alkaline phosphatase (ALP), bone sialopoietin 
      (BSP), osteopontin (OPN), osteocalcin (OC), matrix metalloproteinase-3 (MMP-3) 
      collagen I (Col I) and cyclooxygenase-2 (COX-2) expression by reverse 
      transcription-polymerase chain reaction (RT-PCR). RESULTS: The PDL cell viability 
      showed that the differentiation medium groups were higher than the normal culture 
      groups. The cell morphologies were all expressed as spindle type. The 
      inflammatory markers in the laser-irradiated groups were higher on the first day 
      and decreased on the seventh day (P < 0.05). Osteogenesis markers were highly 
      expressed at different time periods (P < 0.05). The Col I and OPN genes were 
      highly expressed on the first day, and the Col I high expression lasted until the 
      seventh day. The OC gene was highly expressed on the seventh day. The effects of 
      PDL cultured in differential medium and normal medium were the same in the 
      present study. CONCLUSION: A low-dose CO(2) laser continuously irradiating 
      cultured PDL cells can induce osteogenesis and reduce cell inflammatory 
      expression.
CI  - (c) 2022 Association for Dental Sciences of the Republic of China. Publishing 
      services by Elsevier B.V.
FAU - Ho, Chung-Te
AU  - Ho CT
AD  - School of Dentistry, College of Oral Medicine, Chung Shan Medical University, 
      Taichung, Taiwan.
AD  - Orthodontic Department, Chung Shan Medical University Hospital, Taichung, Taiwan.
FAU - Huang, Tsui-Hsein
AU  - Huang TH
AD  - School of Dentistry, College of Oral Medicine, Chung Shan Medical University, 
      Taichung, Taiwan.
AD  - Dental Department, Chung Shan Medical University Hospital, Taichung, Taiwan.
FAU - Kao, Chia-Tze
AU  - Kao CT
AD  - School of Dentistry, College of Oral Medicine, Chung Shan Medical University, 
      Taichung, Taiwan.
AD  - Orthodontic Department, Chung Shan Medical University Hospital, Taichung, Taiwan.
AD  - Dental Department, Chung Shan Medical University Hospital, Taichung, Taiwan.
LA  - eng
PT  - Journal Article
DEP - 20220604
PL  - Netherlands
TA  - J Dent Sci
JT  - Journal of dental sciences
JID - 101293181
PMC - PMC9588823
OTO - NOTNLM
OT  - CO2 laser
OT  - Inflammation
OT  - Osteogenesis
OT  - PDL cell
OT  - Photobiostimulation
OT  - Viability
COIS- All authors have no conflicts of interest relevant to this article.
EDAT- 2022/10/28 06:00
MHDA- 2022/10/28 06:01
PMCR- 2022/06/04
CRDT- 2022/10/27 02:22
PHST- 2022/05/10 00:00 [received]
PHST- 2022/05/22 00:00 [revised]
PHST- 2022/10/27 02:22 [entrez]
PHST- 2022/10/28 06:00 [pubmed]
PHST- 2022/10/28 06:01 [medline]
PHST- 2022/06/04 00:00 [pmc-release]
AID - S1991-7902(22)00110-6 [pii]
AID - 10.1016/j.jds.2022.05.016 [doi]
PST - ppublish
SO  - J Dent Sci. 2022 Oct;17(4):1751-1761. doi: 10.1016/j.jds.2022.05.016. Epub 2022 
      Jun 4.