PMID- 36299604
OWN - NLM
STAT- MEDLINE
DCOM- 20221028
LR  - 20221028
IS  - 1942-0994 (Electronic)
IS  - 1942-0900 (Print)
IS  - 1942-0994 (Linking)
VI  - 2022
DP  - 2022
TI  - Corilagin Restrains NLRP3 Inflammasome Activation and Pyroptosis through the 
      ROS/TXNIP/NLRP3 Pathway to Prevent Inflammation.
PG  - 1652244
LID - 10.1155/2022/1652244 [doi]
LID - 1652244
AB  - Corilagin, a gallotannin, shows excellent antioxidant and anti-inflammatory 
      effects. The NLRP3 inflammasome dysfunction has been implicated in a variety of 
      inflammation diseases. However, it remains unclear how corilagin regulates the 
      NLRP3 inflammasome to relieve gouty arthritis. In this study, bone marrow-derived 
      macrophages (BMDMs) were pretreated with lipopolysaccharide (LPS) and then 
      incubated with NLRP3 inflammasome agonists, such as adenine nucleoside 
      triphosphate (ATP), nigericin, and monosodium urate (MSU) crystals. The MSU 
      crystals were intra-articular injected to induce acute gouty arthritis. Here we 
      showed that corilagin reduced lactate dehydrogenase (LDH) secretion and the 
      proportion of propidium iodide- (PI-)stained cells. Corilagin suppressed the 
      expression of N-terminal of the pyroptosis executive protein gasdermin D 
      (GSDMD-NT). Corilagin restricted caspase-1 p20 and interleukin (IL)-1beta release. 
      Meanwhile, corilagin attenuated ASC oligomerization and speck formation. Our 
      findings confirmed that corilagin diminished NLRP3 inflammasome activation and 
      macrophage pyroptosis. We further discovered that corilagin limited the 
      mitochondrial reactive oxygen species (ROS) production and prevented the 
      interaction between TXNIP and NLRP3, but ROS activator imiquimod could antagonize 
      the inhibitory function of corilagin on NLRP3 inflammasome and macrophage 
      pyroptosis. Additionally, corilagin ameliorated MSU crystals induced joint 
      swelling, inhibited IL-1beta production, and abated macrophage and neutrophil 
      migration into the joint capsule. Collectively, these results demonstrated that 
      corilagin suppressed the ROS/TXNIP/NLRP3 pathway to repress inflammasome 
      activation and pyroptosis and suggest its potential antioxidative role in 
      alleviating NLRP3-dependent gouty arthritis.
CI  - Copyright (c) 2022 Tianyu Luo et al.
FAU - Luo, Tianyu
AU  - Luo T
AD  - Department of Human Anatomy, School of Basic Medical Sciences, Guangzhou 
      University of Chinese Medicine, Guangzhou, Guangdong, China.
AD  - Research Center for Integrative Medicine, School of Basic Medical Sciences, 
      Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China.
FAU - Zhou, Xiaoyi
AU  - Zhou X
AD  - Department of Human Anatomy, School of Basic Medical Sciences, Guangzhou 
      University of Chinese Medicine, Guangzhou, Guangdong, China.
AD  - Research Center for Integrative Medicine, School of Basic Medical Sciences, 
      Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China.
FAU - Qin, Minyan
AU  - Qin M
AD  - Department of Human Anatomy, School of Basic Medical Sciences, Guangzhou 
      University of Chinese Medicine, Guangzhou, Guangdong, China.
AD  - Research Center for Integrative Medicine, School of Basic Medical Sciences, 
      Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China.
FAU - Lin, Yuqing
AU  - Lin Y
AD  - Department of Human Anatomy, School of Basic Medical Sciences, Guangzhou 
      University of Chinese Medicine, Guangzhou, Guangdong, China.
AD  - Research Center for Integrative Medicine, School of Basic Medical Sciences, 
      Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China.
FAU - Lin, Jiefen
AU  - Lin J
AD  - Research Center for Integrative Medicine, School of Basic Medical Sciences, 
      Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China.
FAU - Chen, Guangpei
AU  - Chen G
AD  - Department of Human Anatomy, School of Basic Medical Sciences, Guangzhou 
      University of Chinese Medicine, Guangzhou, Guangdong, China.
FAU - Liu, Aijun
AU  - Liu A
AD  - Department of Human Anatomy, School of Basic Medical Sciences, Guangzhou 
      University of Chinese Medicine, Guangzhou, Guangdong, China.
AD  - Research Center for Integrative Medicine, School of Basic Medical Sciences, 
      Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China.
FAU - Ouyang, Dongyun
AU  - Ouyang D
AD  - Department of Immunobiology, College of Life Science and Technology, Jinan 
      University, Guangzhou, Guangdong, China.
FAU - Chen, Dongfeng
AU  - Chen D
AUID- ORCID: 0000-0003-1263-2374
AD  - Department of Human Anatomy, School of Basic Medical Sciences, Guangzhou 
      University of Chinese Medicine, Guangzhou, Guangdong, China.
AD  - Research Center for Integrative Medicine, School of Basic Medical Sciences, 
      Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China.
FAU - Pan, Hao
AU  - Pan H
AUID- ORCID: 0000-0002-2049-1842
AD  - Department of Human Anatomy, School of Basic Medical Sciences, Guangzhou 
      University of Chinese Medicine, Guangzhou, Guangdong, China.
AD  - Research Center for Integrative Medicine, School of Basic Medical Sciences, 
      Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China.
AD  - Dongguan Institute of Guangzhou University of Chinese Medicine, Dongguan, 
      Guangdong, China.
LA  - eng
PT  - Journal Article
DEP - 20221017
PL  - United States
TA  - Oxid Med Cell Longev
JT  - Oxidative medicine and cellular longevity
JID - 101479826
RN  - 0 (Inflammasomes)
RN  - 0 (NLR Family, Pyrin Domain-Containing 3 Protein)
RN  - 0 (Reactive Oxygen Species)
RN  - 62LOS9TW6D (corilagin)
RN  - 0 (Hydrolyzable Tannins)
RN  - 0 (Lipopolysaccharides)
RN  - 268B43MJ25 (Uric Acid)
RN  - 0 (Antioxidants)
RN  - RRU6GY95IS (Nigericin)
RN  - P1QW714R7M (Imiquimod)
RN  - 36015-30-2 (Propidium)
RN  - 0 (Nucleosides)
RN  - EC 3.4.22.36 (Caspase 1)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Anti-Inflammatory Agents)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - JAC85A2161 (Adenine)
RN  - EC 1.1.- (Lactate Dehydrogenases)
RN  - 0 (TXNIP protein, human)
SB  - IM
MH  - Humans
MH  - *Pyroptosis
MH  - Inflammasomes/metabolism
MH  - NLR Family, Pyrin Domain-Containing 3 Protein/metabolism
MH  - Reactive Oxygen Species/metabolism
MH  - Hydrolyzable Tannins/pharmacology/therapeutic use
MH  - Lipopolysaccharides/pharmacology
MH  - *Arthritis, Gouty/drug therapy/metabolism
MH  - Uric Acid/therapeutic use
MH  - Antioxidants/pharmacology
MH  - Nigericin/pharmacology/therapeutic use
MH  - Imiquimod/pharmacology/therapeutic use
MH  - Propidium/pharmacology/therapeutic use
MH  - Nucleosides/pharmacology
MH  - Caspase 1/metabolism
MH  - Inflammation/drug therapy/metabolism
MH  - Interleukin-1beta/metabolism
MH  - Anti-Inflammatory Agents/pharmacology
MH  - Adenosine Triphosphate/pharmacology
MH  - Adenine/pharmacology
MH  - Lactate Dehydrogenases
PMC - PMC9592212
COIS- All authors declare that they have no competing financial interests.
EDAT- 2022/10/28 06:00
MHDA- 2022/10/29 06:00
PMCR- 2022/10/17
CRDT- 2022/10/27 02:28
PHST- 2022/02/16 00:00 [received]
PHST- 2022/06/22 00:00 [revised]
PHST- 2022/09/24 00:00 [accepted]
PHST- 2022/10/27 02:28 [entrez]
PHST- 2022/10/28 06:00 [pubmed]
PHST- 2022/10/29 06:00 [medline]
PHST- 2022/10/17 00:00 [pmc-release]
AID - 10.1155/2022/1652244 [doi]
PST - epublish
SO  - Oxid Med Cell Longev. 2022 Oct 17;2022:1652244. doi: 10.1155/2022/1652244. 
      eCollection 2022.