PMID- 36300875
OWN - NLM
STAT- MEDLINE
DCOM- 20221216
LR  - 20221222
IS  - 1521-3773 (Electronic)
IS  - 1433-7851 (Linking)
VI  - 61
IP  - 52
DP  - 2022 Dec 23
TI  - An RNA G-Quadruplex Structure within the ADAR 5'UTR Interacts with DHX36 Helicase 
      to Regulate Translation.
PG  - e202203553
LID - 10.1002/anie.202203553 [doi]
AB  - RNA G-quadruplex (rG4) structures in the 5' untranslated region (5'UTR) play 
      crucial roles in fundamental cellular processes. ADAR is an important enzyme that 
      binds to double-strand RNA and accounts for the conversion of Adenosine to 
      Inosine in RNA editing. However, so far there is no report on the formation and 
      regulatory role of rG4 on ADAR expression. Here, we identify and characterize a 
      thermostable rG4 structure within the 5'UTR of the ADAR1 mRNA and demonstrate its 
      formation and inhibitory role on translation in reporter gene and native gene 
      constructs. We reveal rG4-specific helicase DHX36 interacts with this rG4 in 
      vitro and in cells under knockdown and knockout conditions by GTFH 
      (G-quadruplex-triggered fluorogenic hybridization) probes and modulates 
      translation in an rG4-dependent manner. Our results further substantiate the rG4 
      structure-DHX36 protein interaction in cells and highlight rG4 to be a key player 
      in controlling ADAR1 translation.
CI  - (c) 2022 Wiley-VCH GmbH.
FAU - Lyu, Kaixin
AU  - Lyu K
AUID- ORCID: 0000-0002-9330-1296
AD  - Department of Chemistry and State Key Laboratory of Marine Pollution, City 
      University of Hong Kong, Hong Kong SAR, China.
FAU - Chen, Shuo-Bin
AU  - Chen SB
AUID- ORCID: 0000-0001-9118-2185
AD  - School of Pharmaceutical Sciences, Guangdong Provincial Key Laboratory of New 
      Drug Design and Evaluation, Sun Yat-sen University, Guangzhou, 510006, China.
FAU - Chow, Eugene Yui-Ching
AU  - Chow EY
AUID- ORCID: 0000-0002-5575-6724
AD  - School of Life Sciences, and State Key Laboratory of Agrobiotechnology, The 
      Chinese University of Hong Kong, Hong Kong SAR, China.
FAU - Zhao, Haizhou
AU  - Zhao H
AUID- ORCID: 0000-0002-2920-6219
AD  - Department of Chemistry and State Key Laboratory of Marine Pollution, City 
      University of Hong Kong, Hong Kong SAR, China.
FAU - Yuan, Jia-Hao
AU  - Yuan JH
AD  - School of Pharmaceutical Sciences, Guangdong Provincial Key Laboratory of New 
      Drug Design and Evaluation, Sun Yat-sen University, Guangzhou, 510006, China.
FAU - Cai, Meng
AU  - Cai M
AUID- ORCID: 0000-0002-0748-7363
AD  - Shenzhen Research Institute of City University of Hong Kong, Shenzhen, China.
AD  - Department of Biomedical Sciences, College of Veterinary Medicine and Life 
      Sciences, Tung Biomedical Sciences Center, City University of Hong Kong, Hong 
      Kong SAR, China.
FAU - Shi, Jiahai
AU  - Shi J
AUID- ORCID: 0000-0002-6467-8289
AD  - Shenzhen Research Institute of City University of Hong Kong, Shenzhen, China.
AD  - Department of Biomedical Sciences, College of Veterinary Medicine and Life 
      Sciences, Tung Biomedical Sciences Center, City University of Hong Kong, Hong 
      Kong SAR, China.
AD  - Department of Biochemistry, Synthetic Biology Translational Research Programmes, 
      Yong Loo Lin School of Medicine, National University of, Singapore, Singapore.
FAU - Chan, Ting-Fung
AU  - Chan TF
AUID- ORCID: 0000-0002-0489-3884
AD  - School of Life Sciences, and State Key Laboratory of Agrobiotechnology, The 
      Chinese University of Hong Kong, Hong Kong SAR, China.
FAU - Tan, Jia-Heng
AU  - Tan JH
AUID- ORCID: 0000-0002-1612-7482
AD  - School of Pharmaceutical Sciences, Guangdong Provincial Key Laboratory of New 
      Drug Design and Evaluation, Sun Yat-sen University, Guangzhou, 510006, China.
FAU - Kwok, Chun Kit
AU  - Kwok CK
AUID- ORCID: 0000-0001-9175-8543
AD  - Department of Chemistry and State Key Laboratory of Marine Pollution, City 
      University of Hong Kong, Hong Kong SAR, China.
AD  - Shenzhen Research Institute of City University of Hong Kong, Shenzhen, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20221123
PL  - Germany
TA  - Angew Chem Int Ed Engl
JT  - Angewandte Chemie (International ed. in English)
JID - 0370543
RN  - 0 (5' Untranslated Regions)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - 5' Untranslated Regions
MH  - *G-Quadruplexes
MH  - RNA, Messenger/metabolism
OTO - NOTNLM
OT  - ADAR
OT  - DHX36
OT  - Gene Expression
OT  - RNA G-Quadruplex
OT  - Structure-Function Relationship
EDAT- 2022/10/28 06:00
MHDA- 2022/12/17 06:00
CRDT- 2022/10/27 08:24
PHST- 2022/03/08 00:00 [received]
PHST- 2022/10/28 06:00 [pubmed]
PHST- 2022/12/17 06:00 [medline]
PHST- 2022/10/27 08:24 [entrez]
AID - 10.1002/anie.202203553 [doi]
PST - ppublish
SO  - Angew Chem Int Ed Engl. 2022 Dec 23;61(52):e202203553. doi: 
      10.1002/anie.202203553. Epub 2022 Nov 23.