PMID- 36303897
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221029
IS  - 2405-8440 (Print)
IS  - 2405-8440 (Electronic)
IS  - 2405-8440 (Linking)
VI  - 8
IP  - 10
DP  - 2022 Oct
TI  - Antifungal potential of isoespintanol extracted from Oxandra xylopioides diels 
      (Annonaceae) against intrahospital isolations of Candida SPP.
PG  - e11110
LID - 10.1016/j.heliyon.2022.e11110 [doi]
LID - e11110
AB  - The aim of this study was to evaluate the antifungal activity of isoespintanol 
      (ISO) extracted from Oxandra xylopioides Diels (Annonaceae) against clinical 
      isolates of Candida spp. Isoespintanol was obtained from the petroleum benzine 
      extract of the leaves and was identified by nuclear magnetic resonance (NMR) and 
      mass spectrometry (MS). For antifungal activity experiments, the broth 
      microdilution method was used. The results show an inhibitory effect against 
      Candida spp., with minimum inhibitory concentration (MIC) values between 
      450.4-503.3 mug/mL. Furthermore, the inhibitory effect of ISO against fungal 
      biofilms is highlighted, even in some cases, greater than the effect shown by 
      amphotericin B (AFB) and in others, where AFB showed no effect. Assays with 
      fluorescent staining with acridine orange (AO) and ethidium bromide (EB), 
      transmission electron microscopy (TEM), Evans blue, measurement of extracellular 
      pH and leakage of intracellular material, evidenced damage at the level of fungal 
      membranes and general cell damage, when cells were exposed to ISO, compared to 
      untreated cells. The results of this research, serve as the basis for future 
      studies in the establishment of the mechanisms of antifungal action of ISO, which 
      could serve as an adjunct in the treatment of infections by these yeasts.
CI  - (c) 2022 The Author(s).
FAU - Contreras Martinez, Orfa Ines
AU  - Contreras Martinez OI
AD  - Universidad de Cordoba, Facultad de Ciencias Basicas, Monteria, Cordoba, 
      Colombia.
FAU - Ortiz, Alberto Angulo
AU  - Ortiz AA
AD  - Universidad de Cordoba, Facultad de Ciencias Basicas, Monteria, Cordoba, 
      Colombia.
FAU - Patino, Gilmar Santafe
AU  - Patino GS
AD  - Universidad de Cordoba, Facultad de Ciencias Basicas, Monteria, Cordoba, 
      Colombia.
LA  - eng
PT  - Journal Article
DEP - 20221018
PL  - England
TA  - Heliyon
JT  - Heliyon
JID - 101672560
PMC - PMC9593293
OTO - NOTNLM
OT  - Antifungal
OT  - Candida spp.
OT  - Isoespintanol
OT  - Oxandra xylopioides
COIS- The authors declare no conflict of interest.
EDAT- 2022/10/29 06:00
MHDA- 2022/10/29 06:01
PMCR- 2022/10/18
CRDT- 2022/10/28 02:40
PHST- 2021/12/12 00:00 [received]
PHST- 2022/04/24 00:00 [revised]
PHST- 2022/10/11 00:00 [accepted]
PHST- 2022/10/28 02:40 [entrez]
PHST- 2022/10/29 06:00 [pubmed]
PHST- 2022/10/29 06:01 [medline]
PHST- 2022/10/18 00:00 [pmc-release]
AID - S2405-8440(22)02398-2 [pii]
AID - e11110 [pii]
AID - 10.1016/j.heliyon.2022.e11110 [doi]
PST - epublish
SO  - Heliyon. 2022 Oct 18;8(10):e11110. doi: 10.1016/j.heliyon.2022.e11110. 
      eCollection 2022 Oct.