PMID- 3630594
OWN - NLM
STAT- MEDLINE
DCOM- 19870929
LR  - 20190814
IS  - 0001-5172 (Print)
IS  - 0001-5172 (Linking)
VI  - 31
IP  - 6
DP  - 1987 Aug
TI  - Differences and similarities between human and rabbit neutrophil granulocyte 
      responses in vitro: the effects of zymosan-activated plasma, phorbol myristate 
      acetate and n-formyl-methionyl-leucyl-phenylalanine.
PG  - 491-8
AB  - The amount of reactive oxygen intermediates (ROI) generated by activated 
      polymorphonuclear neutrophils (PMN), as well as the closeness of contact between 
      PMN and vessel wall, may determine whether PMN activators will induce the adult 
      respiratory distress syndrome. We examined the ROI-generating and aggregating 
      effects of zymosan activated plasma (ZAP), phorbol myristate acetate (PMA) and 
      n-formyl-methionyl-leucyl-phenylalanine (FMLP), on isolated human and rabbit PMN. 
      PMA, after a short lag phase, induced a large and long-lasting increase in ROI 
      generation. The initial peak response was higher and more rapid in human than in 
      rabbit cells. The reaction to FMLP occurred almost instantaneously, but was much 
      weaker than that to PMA, and ROI generation returned to near baseline in less 
      than 10 min. No species difference was seen. ZAP caused an FMLP-like ROI response 
      in human cells, whereas no response was observed in rabbit PMN. PMN aggregation 
      was induced by all three activators, most markedly by PMA. No species difference 
      was detected for PMA; FMLP gave a stronger aggregation of rabbit than of human 
      PMN, however, while the opposite was true for ZAP. In conclusion, ZAP was a 
      potent stimulus for PMN aggregation, but had modest (or no) effects on the 
      production of ROI. Marked differences between human and rabbit PMN responses were 
      observed.
FAU - Opdahl, H
AU  - Opdahl H
FAU - Benestad, H B
AU  - Benestad HB
FAU - Nicolaysen, G
AU  - Nicolaysen G
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Acta Anaesthesiol Scand
JT  - Acta anaesthesiologica Scandinavica
JID - 0370270
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - 9010-72-4 (Zymosan)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Animals
MH  - Cell Aggregation/drug effects
MH  - Granulocytes/*drug effects
MH  - Humans
MH  - In Vitro Techniques
MH  - Luminescent Measurements
MH  - N-Formylmethionine Leucyl-Phenylalanine/*pharmacology
MH  - Oxygen Consumption/drug effects
MH  - Plasma/drug effects/*physiology
MH  - Rabbits
MH  - Species Specificity
MH  - Tetradecanoylphorbol Acetate/*pharmacology
MH  - Zymosan/*pharmacology
EDAT- 1987/08/01 00:00
MHDA- 1987/08/01 00:01
CRDT- 1987/08/01 00:00
PHST- 1987/08/01 00:00 [pubmed]
PHST- 1987/08/01 00:01 [medline]
PHST- 1987/08/01 00:00 [entrez]
AID - 10.1111/j.1399-6576.1987.tb02609.x [doi]
PST - ppublish
SO  - Acta Anaesthesiol Scand. 1987 Aug;31(6):491-8. doi: 
      10.1111/j.1399-6576.1987.tb02609.x.