PMID- 36306716
OWN - NLM
STAT- MEDLINE
DCOM- 20221219
LR  - 20221222
IS  - 1873-2232 (Electronic)
IS  - 0378-4320 (Linking)
VI  - 247
DP  - 2022 Dec
TI  - Effects of mitoquinone (MitoQ) supplementation during boar semen cryopreservation 
      on sperm quality, antioxidant status and mitochondrial proteomics.
PG  - 107099
LID - S0378-4320(22)00178-6 [pii]
LID - 10.1016/j.anireprosci.2022.107099 [doi]
AB  - Mitochondria are the most important organelles and the main reactive oxygen 
      species producers in spermatozoa. The objective of this study was to investigate 
      the effects of mitochondria-targeted antioxidant mitoquinone (MitoQ) 
      supplementation in boar semen extender during cryopreservation on sperm quality, 
      antioxidant status and the changes of sperm mitochondrial proteomic profile. 
      Semen collected from 10 Large White boars was cryopreserved in lactose-egg yolk 
      extender supplemented with various concentrations of MitoQ (0, 5, 10, 20 and 
      40 muM). After thawing, sperm characteristics, antioxidant status and the 
      abundance of hexose transporters (GLUT 3 and 8) were analyzed. The comprehensive 
      mitochondrial proteomic profiling was performed on spermatozoa in the control and 
      MitoQ(10) groups. Supplementation with 10 muM of MitoQ resulted in the highest 
      post-thaw sperm motility and kinematics. Sperm quality, antioxidant capacity and 
      glucose transporter abundance of frozen-thawed boar sperm were also elevated in 
      the MitoQ(10) group. Excessive MitoQ (40 muM) supplementation induced a reduction 
      of sperm motility parameters, sperm quality and antioxidant status and the 
      abundance of GLUT3 and 8 proteins. A total of 189 proteins were defied as 
      differentially expressed proteins (DEPs) using fold change (FC) > 1.2 with 
      P < 0.05, and 33 of them were dramatically (FC > 1.5) regulated by MitoQ. These 
      DEPs are mainly involved in sperm motility, energy metabolism and oxidative 
      phosphorylation. Our data suggest that the beneficial effect of MitoQ on 
      cryopreserved boar semen is achieved by regulating sperm antioxidant capacity and 
      the mitochondrial proteins related to motility and metabolism.
CI  - Copyright (c) 2022 Elsevier B.V. All rights reserved.
FAU - Shi, Lei
AU  - Shi L
AD  - College of Animal Science, Shanxi Agricultural University, Taigu 030801, PR 
      China; Lab of Animal Reproductive Biotechnology, Shanxi Agricultural University, 
      Taigu 030801, PR China.
FAU - Zhang, Yan
AU  - Zhang Y
AD  - College of Animal Science, Shanxi Agricultural University, Taigu 030801, PR 
      China.
FAU - Huang, Xiaoyu
AU  - Huang X
AD  - College of Animal Science, Shanxi Agricultural University, Taigu 030801, PR 
      China.
FAU - Shi, Mingyue
AU  - Shi M
AD  - College of Animal Science, Shanxi Agricultural University, Taigu 030801, PR 
      China.
FAU - Sun, Di
AU  - Sun D
AD  - College of Animal Science, Shanxi Agricultural University, Taigu 030801, PR 
      China.
FAU - Zhang, Yanwei
AU  - Zhang Y
AD  - College of Animal Science, Shanxi Agricultural University, Taigu 030801, PR 
      China.
FAU - Li, Wenxin
AU  - Li W
AD  - College of Animal Science, Shanxi Agricultural University, Taigu 030801, PR 
      China.
FAU - Jin, Tianyu
AU  - Jin T
AD  - College of Animal Science, Shanxi Agricultural University, Taigu 030801, PR 
      China.
FAU - Feng, Jingjuan
AU  - Feng J
AD  - College of Animal Science, Shanxi Agricultural University, Taigu 030801, PR 
      China.
FAU - Xing, Jiandong
AU  - Xing J
AD  - College of Animal Science, Shanxi Agricultural University, Taigu 030801, PR 
      China.
FAU - Li, Bugao
AU  - Li B
AD  - College of Animal Science, Shanxi Agricultural University, Taigu 030801, PR 
      China.
FAU - Cao, Guoqing
AU  - Cao G
AD  - College of Animal Science, Shanxi Agricultural University, Taigu 030801, PR 
      China. Electronic address: anniecao710502@aliyun.com.
LA  - eng
PT  - Journal Article
DEP - 20221020
PL  - Netherlands
TA  - Anim Reprod Sci
JT  - Animal reproduction science
JID - 7807205
RN  - 47BYS17IY0 (mitoquinone)
RN  - 0 (Antioxidants)
RN  - 0 (Cryoprotective Agents)
SB  - IM
MH  - Male
MH  - Swine
MH  - Animals
MH  - *Semen
MH  - *Semen Preservation/veterinary/methods
MH  - Sperm Motility
MH  - Antioxidants/pharmacology
MH  - Proteomics
MH  - Cryopreservation/veterinary/methods
MH  - Spermatozoa/physiology
MH  - Semen Analysis/veterinary
MH  - Mitochondria/physiology
MH  - Dietary Supplements
MH  - Cryoprotective Agents/pharmacology
OTO - NOTNLM
OT  - Antioxidation
OT  - Boar semen
OT  - Cryopreservation
OT  - Mitochondrial proteomics
OT  - Mitoquinone
COIS- Conflicts of interest The authors declared that there is no conflict of interest.
EDAT- 2022/10/29 06:00
MHDA- 2022/12/20 06:00
CRDT- 2022/10/28 18:28
PHST- 2022/08/23 00:00 [received]
PHST- 2022/10/17 00:00 [revised]
PHST- 2022/10/18 00:00 [accepted]
PHST- 2022/10/29 06:00 [pubmed]
PHST- 2022/12/20 06:00 [medline]
PHST- 2022/10/28 18:28 [entrez]
AID - S0378-4320(22)00178-6 [pii]
AID - 10.1016/j.anireprosci.2022.107099 [doi]
PST - ppublish
SO  - Anim Reprod Sci. 2022 Dec;247:107099. doi: 10.1016/j.anireprosci.2022.107099. 
      Epub 2022 Oct 20.