PMID- 36306915
OWN - NLM
STAT- MEDLINE
DCOM- 20221221
LR  - 20231213
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
VI  - 224
DP  - 2023 Jan 1
TI  - Effect of proanthocyanidins on protein composition, conformational structure, IgE 
      binding capacities and functional properties in soybean protein.
PG  - 881-892
LID - S0141-8130(22)02416-3 [pii]
LID - 10.1016/j.ijbiomac.2022.10.174 [doi]
AB  - This study was performed to determine the crosslinking formed by 
      proanthocyanidins (PC) with respect to IgE binding capacities, functionality, 
      structure and composition of soybean protein (SPI) following the alkali treatment 
      at 60-100  degrees C. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis 
      (SDS-PAGE) showed the formation of >180 kDa polymers, resulting from the 
      formation of SPI-PC conjugates and protein cross-links. Structural analyses 
      demonstrated that SPI-PC conjugates exhibited structural changes to unfold 
      proteins and increase molecular flexibility. Liquid chromatography coupled to 
      tandem mass spectrometry (LC/MS-MS) showed a decrease in unique protein and 
      peptide numbers as well as major allergen and dominant epitopes abundance. When 
      SPI was treated with PC under the alkali treatment at 80  degrees C, it exhibited a 
      maximum reduction (68.8 %) in the immunoglobulin E (IgE) binding capacity and a 
      maximum increase in DPPH radical scavenging activities (6.11-fold), 
      ABTS + radical scavenging activities (4.80-fold), foaming stability (6.1 %) and 
      emulsifying activity (27.3 %), compared to the control SPI. Overall, this study 
      demonstrates that alkali treatment at 60-100  degrees C to form SPI-PC conjugates has 
      potential applications for producing hypoallergenic soybean products with the 
      desired functionality, most especially from alkali treatment at 80  degrees C. Moreover, 
      the addition of PC pronouncedly alleviates the undesirable functional properties 
      in heated SPI.
CI  - Copyright (c) 2022 Elsevier B.V. All rights reserved.
FAU - Pi, Xiaowen
AU  - Pi X
AD  - Northeast Agricultural University, Harbin, Heilongjiang 150030, China.
FAU - Liu, Jiafei
AU  - Liu J
AD  - Northeast Agricultural University, Harbin, Heilongjiang 150030, China.
FAU - Sun, Yuxue
AU  - Sun Y
AD  - Northeast Agricultural University, Harbin, Heilongjiang 150030, China; Key 
      Laboratory of Soybean Biology of Chinese Education Ministry, Harbin 150030, 
      China.
FAU - Ban, Qingfeng
AU  - Ban Q
AD  - Northeast Agricultural University, Harbin, Heilongjiang 150030, China.
FAU - Liang, Shuxia
AU  - Liang S
AD  - Northeast Agricultural University, Harbin, Heilongjiang 150030, China; Jiangsu 
      DAISY FSMP Co., Ltd, Nantong, Jiangsu 226133, China.
FAU - Cheng, Jianjun
AU  - Cheng J
AD  - Northeast Agricultural University, Harbin, Heilongjiang 150030, China. Electronic 
      address: jjcheng@neau.edu.cn.
FAU - Guo, Mingruo
AU  - Guo M
AD  - Department of Nutrition and Food Science, College of Agriculture and Life 
      Sciences, University of Vermont, Burlington 05405, United States. Electronic 
      address: mguo@uvm.edu.
LA  - eng
PT  - Journal Article
DEP - 20221025
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - 0 (Soybean Proteins)
RN  - 0 (Proanthocyanidins)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - *Soybean Proteins/chemistry
MH  - *Proanthocyanidins
MH  - Immunoglobulin E
MH  - Glycine max/chemistry
MH  - Hot Temperature
OTO - NOTNLM
OT  - Composition
OT  - Functionality
OT  - IgE binding capacities
OT  - Proanthocyanidins
OT  - Soybean protein
OT  - Structure
COIS- Declaration of competing interest All authors declare no conflict of interest.
EDAT- 2022/10/29 06:00
MHDA- 2022/12/22 06:00
CRDT- 2022/10/28 19:24
PHST- 2022/08/30 00:00 [received]
PHST- 2022/10/12 00:00 [revised]
PHST- 2022/10/20 00:00 [accepted]
PHST- 2022/10/29 06:00 [pubmed]
PHST- 2022/12/22 06:00 [medline]
PHST- 2022/10/28 19:24 [entrez]
AID - S0141-8130(22)02416-3 [pii]
AID - 10.1016/j.ijbiomac.2022.10.174 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2023 Jan 1;224:881-892. doi: 10.1016/j.ijbiomac.2022.10.174. 
      Epub 2022 Oct 25.