PMID- 36308623
OWN - NLM
STAT- MEDLINE
DCOM- 20230227
LR  - 20230308
IS  - 1573-675X (Electronic)
IS  - 1360-8185 (Linking)
VI  - 28
IP  - 1-2
DP  - 2023 Feb
TI  - Exploration of microRNA-106b-5p as a therapeutic target in intervertebral disc 
      degeneration: a preclinical study.
PG  - 199-209
LID - 10.1007/s10495-022-01773-6 [doi]
AB  - MicroRNA (miRNA) has emerge as a vital regulator in the pathogenesis of 
      intervertebral disc degeneration (IDD). However, miR-106b-5p expression in the 
      human nucleus pulposus (NP) and potential mechanisms remain to be elucidated. In 
      this study, the aim was to verify the potential therapeutic mechanisms of 
      miR-106b-5p for IDD. Key miRNAs were screened for in degenerative and normal 
      human intervertebral disc samples. qRT-PCR and fluorescence in situ hybridization 
      (FISH) were used to verify the miR-106b-5p differential expression. The targeting 
      link between miR-106b-5p and Sirtuin 2 (SIRT2) was identified using the 
      luciferase reporter assay and bioinformatics. Flow cytometry, EdU method, and 
      cell scratching were all performed to determine the NP cell function and IDD 
      models were constructed for in vivo experiments. SIRT2, MMP13, ADAMTS5, Col II, 
      Aggrecan, Ras, ERK1/2, and p-ERK1/2 protein levels were assayed by western 
      blotting. Overexpression of miR-106b-5p in NP cells decreased cell growth, 
      induced apoptosis, hindered extracellular matrix formation, and increased the 
      expression of matrix-degrading enzymes through the SIRT2/MAPK/ERK signaling 
      pathway. Importantly, intradiscal delivery of antagomiR-106b-5p significantly 
      attenuated IDD development. Our findings demonstrate that targeting miR-106b-5p 
      in intervertebral disc has therapeutic effects on IDD.
CI  - (c) 2022. The Author(s), under exclusive licence to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Meng, Dihua
AU  - Meng D
AD  - Division of Spine Surgery, The First Affiliated Hospital of Guangxi Medical 
      University, 6 Shuangyong Road, 530021, Nanning, Guangxi Zhuang Autonomous Region, 
      China.
FAU - Chen, Weiyou
AU  - Chen W
AD  - Division of Spine Surgery, The First Affiliated Hospital of Guangxi Medical 
      University, 6 Shuangyong Road, 530021, Nanning, Guangxi Zhuang Autonomous Region, 
      China.
FAU - Pan, Cheng
AU  - Pan C
AD  - Division of Spine Surgery, The First Affiliated Hospital of Guangxi Medical 
      University, 6 Shuangyong Road, 530021, Nanning, Guangxi Zhuang Autonomous Region, 
      China.
FAU - Yang, Kunxue
AU  - Yang K
AD  - Division of Spine Surgery, The First Affiliated Hospital of Guangxi Medical 
      University, 6 Shuangyong Road, 530021, Nanning, Guangxi Zhuang Autonomous Region, 
      China.
FAU - Guan, Yewen
AU  - Guan Y
AD  - Division of Spine Surgery, The First Affiliated Hospital of Guangxi Medical 
      University, 6 Shuangyong Road, 530021, Nanning, Guangxi Zhuang Autonomous Region, 
      China.
FAU - Wang, Jiaqi
AU  - Wang J
AD  - Division of Spine Surgery, The First Affiliated Hospital of Guangxi Medical 
      University, 6 Shuangyong Road, 530021, Nanning, Guangxi Zhuang Autonomous Region, 
      China.
FAU - Moro, Abu
AU  - Moro A
AD  - Division of Spine Surgery, The First Affiliated Hospital of Guangxi Medical 
      University, 6 Shuangyong Road, 530021, Nanning, Guangxi Zhuang Autonomous Region, 
      China.
FAU - Wei, Qingjun
AU  - Wei Q
AD  - Department of Orthopaedic Surgery, The First Affiliated Hospital of Guangxi 
      Medical University, 530021, Nanning, Guangxi Zhuang Autonomous Region, China.
FAU - Jiang, Hua
AU  - Jiang H
AD  - Division of Spine Surgery, The First Affiliated Hospital of Guangxi Medical 
      University, 6 Shuangyong Road, 530021, Nanning, Guangxi Zhuang Autonomous Region, 
      China. jianghua@gxmu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20221029
PL  - Netherlands
TA  - Apoptosis
JT  - Apoptosis : an international journal on programmed cell death
JID - 9712129
RN  - EC 3.5.1.- (Sirtuin 2)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Humans
MH  - *Intervertebral Disc Degeneration/pathology
MH  - In Situ Hybridization, Fluorescence
MH  - Sirtuin 2/genetics
MH  - Apoptosis
MH  - *MicroRNAs/genetics
MH  - *Intervertebral Disc/metabolism
OTO - NOTNLM
OT  - Intervertebral disc degeneration
OT  - MAPK
OT  - Sirtuin 2
OT  - miR-106b-5p
EDAT- 2022/10/30 06:00
MHDA- 2023/03/03 06:00
CRDT- 2022/10/29 11:18
PHST- 2022/09/17 00:00 [accepted]
PHST- 2022/10/30 06:00 [pubmed]
PHST- 2023/03/03 06:00 [medline]
PHST- 2022/10/29 11:18 [entrez]
AID - 10.1007/s10495-022-01773-6 [pii]
AID - 10.1007/s10495-022-01773-6 [doi]
PST - ppublish
SO  - Apoptosis. 2023 Feb;28(1-2):199-209. doi: 10.1007/s10495-022-01773-6. Epub 2022 
      Oct 29.