PMID- 36309684
OWN - NLM
STAT- MEDLINE
DCOM- 20221101
LR  - 20221228
IS  - 1756-6606 (Electronic)
IS  - 1756-6606 (Linking)
VI  - 15
IP  - 1
DP  - 2022 Oct 29
TI  - Preferential subcortical collateral projections of pedunculopontine 
      nucleus-targeting cortical pyramidal neurons revealed by brain-wide single fiber 
      tracing.
PG  - 88
LID - 10.1186/s13041-022-00975-y [doi]
LID - 88
AB  - The pedunculopontine nucleus (PPN) is a heterogeneous midbrain structure involved 
      in various brain functions, such as motor control, learning, reward, and sleep. 
      Previous studies using conventional tracers have shown that the PPN receives 
      extensive afferent inputs from various cortical areas. To examine how these 
      cortical axons make collateral projections to other subcortical areas, we used a 
      dual-viral injection strategy to sparsely label PPN-targeting cortical pyramidal 
      neurons in CaMKIIalpha-Cre transgenic mice. Using a high-speed volumetric imaging 
      with on-the-fly-scan and Readout (VISoR) technique, we visualized brain-wide 
      axonal projections of individual PPN-targeting neurons from several cortical 
      areas, including the prelimbic region (PL), anterior cingulate area (ACA) and 
      secondary motor cortex (MOs). We found that each PPN-projecting neuron had a 
      unique profile of collateralization, with some subcortical areas being 
      preferential targets. In particular, PPN-projecting neurons from all three traced 
      cortical areas exhibited common preferential collateralization to several nuclei, 
      with most neurons targeting the striatum (STR), lateral hypothalamic area (LHA) 
      and periaqueductal gray (PAG), and a substantial portion of neurons also 
      targeting the zona incerta (ZI), median raphe nucleus (MRN) and substantia nigra 
      pars reticulata (SNr). Meanwhile, very specific collateralization patterns were 
      found for other nuclei, including the intermediate reticular nucleus (IRN), 
      parvicellular reticular nucleus (PARN) and gigantocellular reticular nucleus 
      (GRN), which receive collateral inputs almost exclusively from the MOs. These 
      observations provide potential anatomical mechanisms for cortical neurons to 
      coordinate the PPN with other subcortical areas in performing different 
      physiological functions.
CI  - (c) 2022. The Author(s).
FAU - Liu, Qiao-Qiong
AU  - Liu QQ
AUID- ORCID: 0000-0002-9854-5679
AD  - Division of Life Sciences and Medicine, University of Science and Technology of 
      China, Hefei, 230026, Anhui, China.
FAU - Cheng, Yu-Xiao
AU  - Cheng YX
AD  - Division of Life Sciences and Medicine, University of Science and Technology of 
      China, Hefei, 230026, Anhui, China.
FAU - Jing, Qi
AU  - Jing Q
AD  - Division of Life Sciences and Medicine, University of Science and Technology of 
      China, Hefei, 230026, Anhui, China.
FAU - Zhang, Ke-Ming
AU  - Zhang KM
AD  - Division of Life Sciences and Medicine, University of Science and Technology of 
      China, Hefei, 230026, Anhui, China.
FAU - Ding, Lu-Feng
AU  - Ding LF
AD  - Interdisciplinary Center for Brain Information, The Brain Cognition and Brain 
      Disease Institute, Shenzhen Institute of Advanced Technology, Chinese Academy of 
      Sciences, Shenzhen, 518055, Guangdong, China.
FAU - Fan, Xiao-Wei
AU  - Fan XW
AD  - Interdisciplinary Center for Brain Information, The Brain Cognition and Brain 
      Disease Institute, Shenzhen Institute of Advanced Technology, Chinese Academy of 
      Sciences, Shenzhen, 518055, Guangdong, China.
FAU - Jia, Chun-Hui
AU  - Jia CH
AD  - Division of Life Sciences and Medicine, University of Science and Technology of 
      China, Hefei, 230026, Anhui, China.
FAU - Xu, Fang
AU  - Xu F
AD  - Interdisciplinary Center for Brain Information, The Brain Cognition and Brain 
      Disease Institute, Shenzhen Institute of Advanced Technology, Chinese Academy of 
      Sciences, Shenzhen, 518055, Guangdong, China.
FAU - Bi, Guo-Qiang
AU  - Bi GQ
AUID- ORCID: 0000-0001-8735-3818
AD  - Division of Life Sciences and Medicine, University of Science and Technology of 
      China, Hefei, 230026, Anhui, China. gqbi@ustc.edu.cn.
AD  - Interdisciplinary Center for Brain Information, The Brain Cognition and Brain 
      Disease Institute, Shenzhen Institute of Advanced Technology, Chinese Academy of 
      Sciences, Shenzhen, 518055, Guangdong, China. gqbi@ustc.edu.cn.
FAU - Lau, Pak-Ming
AU  - Lau PM
AD  - Division of Life Sciences and Medicine, University of Science and Technology of 
      China, Hefei, 230026, Anhui, China. plau@ustc.edu.cn.
AD  - Interdisciplinary Center for Brain Information, The Brain Cognition and Brain 
      Disease Institute, Shenzhen Institute of Advanced Technology, Chinese Academy of 
      Sciences, Shenzhen, 518055, Guangdong, China. plau@ustc.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20221029
PL  - England
TA  - Mol Brain
JT  - Molecular brain
JID - 101468876
SB  - IM
MH  - Animals
MH  - Mice
MH  - *Brain/physiology
MH  - Pyramidal Cells
MH  - Medulla Oblongata
MH  - Periaqueductal Gray
MH  - *Motor Cortex
PMC - PMC9618196
OTO - NOTNLM
OT  - Anterior cingulate area
OT  - CaMKIIalpha-positive neuron
OT  - Collateral projection
OT  - Motor cortex
OT  - Pedunculopontine nucleus
OT  - Prelimbic region
OT  - VISoR whole-brain imaging
COIS- The authors declare that they have no competing interests.
EDAT- 2022/10/31 06:00
MHDA- 2022/11/02 06:00
PMCR- 2022/10/29
CRDT- 2022/10/30 01:07
PHST- 2022/08/23 00:00 [received]
PHST- 2022/10/24 00:00 [accepted]
PHST- 2022/10/30 01:07 [entrez]
PHST- 2022/10/31 06:00 [pubmed]
PHST- 2022/11/02 06:00 [medline]
PHST- 2022/10/29 00:00 [pmc-release]
AID - 10.1186/s13041-022-00975-y [pii]
AID - 975 [pii]
AID - 10.1186/s13041-022-00975-y [doi]
PST - epublish
SO  - Mol Brain. 2022 Oct 29;15(1):88. doi: 10.1186/s13041-022-00975-y.