PMID- 36312973
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231102
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 13
DP  - 2022
TI  - Soil bacterial community is more sensitive than fungal community to canopy 
      nitrogen deposition and understory removal in a Chinese fir plantation.
PG  - 1015936
LID - 10.3389/fmicb.2022.1015936 [doi]
LID - 1015936
AB  - Soil microorganisms are key regulators for plant growth and ecosystem health of 
      forest ecosystem. Although previous research has demonstrated that soil 
      microorganisms are greatly affected by understory nitrogen (N) addition, little 
      is known about the effects of canopy N addition (CNA) and understory management 
      on soil microorganisms in forests. In this study, we conducted a full designed 
      field experiment with four treatments: CNA (25 kg N ha(-1) year(-1)), understory 
      removal (UR), canopy N addition, and understory removal (CNAUR) (25 kg N ha(-1) 
      year(-1)), and control in a Chinese fir plantation. High-throughput sequencing 
      and qPCR techniques were used to determine the abundance, diversity, and 
      composition of bacterial and fungal communities in three soil layers. Our results 
      showed that CNA increased bacterial diversity in the 10-20 cm soil layer but 
      decreased bacterial abundance in the 20-40 cm soil layer and fungal diversity in 
      the 0-10 cm soil layer. UR increased bacterial abundance only in the 20-40 cm 
      soil layer. CNA, not UR significantly altered the compositions of soil bacterial 
      and fungal community compositions, especially in the 0-20 cm soil layer. CNA 
      sharply reduced the relative abundance of copiotrophic taxa (i.e., taxa in the 
      bacterial phylum Proteobacteria and the orders Eurotiales and Helotiales in the 
      fungal phylum Ascomycota) but increased the relative abundance of oligotrophic 
      taxa (i.e., in the bacterial phylum Verrucomicrobia). RDA analysis revealed that 
      soil pH, DON, and DOC were the main factors associated with the variation in 
      bacterial and fungal communities. Our findings suggest that short-term CNA 
      changes both soil bacterial and fungal communities, with stronger responses in 
      the surface and middle soil than in the deep soil layer, and that UR may enhance 
      this effect on the soil bacterial abundance. This study improves our 
      understanding of soil microorganisms in plantations managed with understory 
      removal and that experience increases in N deposition.
CI  - Copyright (c) 2022 Xi, Jin and Wu.
FAU - Xi, Dan
AU  - Xi D
AD  - Lushan Botanical Garden, Chinese Academy of Sciences, Jiujiang, China.
AD  - College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, China.
FAU - Jin, Shaofei
AU  - Jin S
AD  - Department of Geography, Minjiang University, Fuzhou, China.
FAU - Wu, Jianping
AU  - Wu J
AD  - Yunnan Key Laboratory of Plant Reproductive Adaptation and Evolutionary Ecology, 
      Institute of Biodiversity, Yunnan University, Kunming, China.
AD  - Key Laboratory of Soil Ecology and Health in Universities of Yunnan Province, 
      School of Ecology and Environmental Sciences, Yunnan University, Kunming, China.
LA  - eng
PT  - Journal Article
DEP - 20221012
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC9597510
OTO - NOTNLM
OT  - Chinese fir plantation
OT  - bacterial diversity
OT  - canopy N deposition
OT  - microbial community
OT  - understory removal
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/11/01 06:00
MHDA- 2022/11/01 06:01
PMCR- 2022/10/12
CRDT- 2022/10/31 04:48
PHST- 2022/08/10 00:00 [received]
PHST- 2022/09/20 00:00 [accepted]
PHST- 2022/10/31 04:48 [entrez]
PHST- 2022/11/01 06:00 [pubmed]
PHST- 2022/11/01 06:01 [medline]
PHST- 2022/10/12 00:00 [pmc-release]
AID - 10.3389/fmicb.2022.1015936 [doi]
PST - epublish
SO  - Front Microbiol. 2022 Oct 12;13:1015936. doi: 10.3389/fmicb.2022.1015936. 
      eCollection 2022.