PMID- 36314003
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221102
IS  - 2296-858X (Print)
IS  - 2296-858X (Electronic)
IS  - 2296-858X (Linking)
VI  - 9
DP  - 2022
TI  - Immunomodulatory potential of secretome from cartilage cells and mesenchymal 
      stromal cells in an arthritic context: From predictive fiction toward reality.
PG  - 992386
LID - 10.3389/fmed.2022.992386 [doi]
LID - 992386
AB  - The purpose of the present study is to predict by bioinformatics the activity of 
      the extracellular vesicle (EV)-embedded micro RNA (miRNAs) secreted by cartilage 
      cells (CCs), adipose tissue-derived- (ASCs), and bone marrow-derived stem cells 
      (BMSCs) and verify their immunomodulatory potential supporting our bioinformatics 
      findings to optimize the autologous cell-based therapeutic strategies for 
      osteoarthritis (OA) management. Cells were isolated from surgical waste tissues 
      of three patients who underwent total hip replacement, expanded and the EVs were 
      collected. The expression of EV-embedded miRNA was evaluated with the QuantStudio 
      12 K Flex OpenArray((R)) platform. Mientournet and ingenuity pathway analysis (IPA) 
      were used for validated target prediction analysis and to identify miRNAs 
      involved in OA and inflammation. Cells shared the expression of 325 miRNAs 
      embedded in EVs and differed for the expression of a small number of them. 
      Mienturnet revealed no results for miRNAs selectively expressed by ASCs, whereas 
      miRNA expressed by CCs and BMSCs were putatively involved in the modulation of 
      cell cycle, senescence, apoptosis, Wingless and Int-1 (Wnt), transforming growth 
      factor beta (TGFbeta), vascular endothelial growth factor (VEGF), Notch, Hippo, 
      tumor necrosis factor alpha (TNFalpha), interleukin 1 beta (IL-1beta), insulin like 
      growth factor 1 (IGF-1), RUNX family transcription factor 2 (RUNX2), and 
      endochondral ossification pathways. Cartilage homeostasis, macrophages and T 
      cells activity and inflammatory mediators were identified by IPA as targets of 
      the miRNAs found in all the cell populations. Co-culture tests on macrophages and 
      T cells confirmed the immuno-modulatory ability of CCs, ASCs, and BMSCs. The 
      study findings support the rationale behind the use of cell-based therapy for the 
      treatment of OA.
CI  - Copyright (c) 2022 Colombini, Libonati, Lopa, Ragni, De Luca, Zagra, Sinigaglia, 
      Moretti and de Girolamo.
FAU - Colombini, Alessandra
AU  - Colombini A
AD  - Laboratorio di Biotecnologie Applicate all'Ortopedia, IRCCS Istituto Ortopedico 
      Galeazzi, Milan, Italy.
FAU - Libonati, Francesca
AU  - Libonati F
AD  - Laboratorio di Biotecnologie Applicate all'Ortopedia, IRCCS Istituto Ortopedico 
      Galeazzi, Milan, Italy.
FAU - Lopa, Silvia
AU  - Lopa S
AD  - Cell and Tissue Engineering Laboratory, IRCCS Istituto Ortopedico Galeazzi, 
      Milan, Italy.
FAU - Ragni, Enrico
AU  - Ragni E
AD  - Laboratorio di Biotecnologie Applicate all'Ortopedia, IRCCS Istituto Ortopedico 
      Galeazzi, Milan, Italy.
FAU - De Luca, Paola
AU  - De Luca P
AD  - Laboratorio di Biotecnologie Applicate all'Ortopedia, IRCCS Istituto Ortopedico 
      Galeazzi, Milan, Italy.
FAU - Zagra, Luigi
AU  - Zagra L
AD  - Hip Department, IRCCS Istituto Ortopedico Galeazzi, Milan, Italy.
FAU - Sinigaglia, Federico
AU  - Sinigaglia F
AD  - Laboratorio di Biotecnologie Applicate all'Ortopedia, IRCCS Istituto Ortopedico 
      Galeazzi, Milan, Italy.
FAU - Moretti, Matteo
AU  - Moretti M
AD  - Cell and Tissue Engineering Laboratory, IRCCS Istituto Ortopedico Galeazzi, 
      Milan, Italy.
AD  - Regenerative Medicine Technologies Lab, Laboratories for Translational Research 
      (LRT), Ente Ospedaliero Cantonale, Bellinzona, Switzerland.
AD  - Department of Surgery, Service of Orthopaedics and Traumatology, Ente Ospedaliero 
      Cantonale, Lugano, Switzerland.
AD  - Faculty of Biomedical Sciences, Euler Institute, USI, Lugano, Switzerland.
FAU - de Girolamo, Laura
AU  - de Girolamo L
AD  - Laboratorio di Biotecnologie Applicate all'Ortopedia, IRCCS Istituto Ortopedico 
      Galeazzi, Milan, Italy.
LA  - eng
PT  - Journal Article
DEP - 20221012
PL  - Switzerland
TA  - Front Med (Lausanne)
JT  - Frontiers in medicine
JID - 101648047
PMC - PMC9596769
OTO - NOTNLM
OT  - adipose stem cells
OT  - bone marrow stem cells
OT  - cartilage cells
OT  - early osteoarthritis
OT  - immunomodulation
OT  - miRNAs
OT  - secretome
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/11/01 06:00
MHDA- 2022/11/01 06:01
PMCR- 2022/10/12
CRDT- 2022/10/31 05:10
PHST- 2022/07/12 00:00 [received]
PHST- 2022/09/16 00:00 [accepted]
PHST- 2022/10/31 05:10 [entrez]
PHST- 2022/11/01 06:00 [pubmed]
PHST- 2022/11/01 06:01 [medline]
PHST- 2022/10/12 00:00 [pmc-release]
AID - 10.3389/fmed.2022.992386 [doi]
PST - epublish
SO  - Front Med (Lausanne). 2022 Oct 12;9:992386. doi: 10.3389/fmed.2022.992386. 
      eCollection 2022.