PMID- 36314140
OWN - NLM
STAT- MEDLINE
DCOM- 20230116
LR  - 20230116
IS  - 1365-2591 (Electronic)
IS  - 0143-2885 (Linking)
VI  - 56
IP  - 2
DP  - 2023 Feb
TI  - Selenium ameliorates inflammation by decreasing autophagic flux and 
      mitogen-activated protein kinase signalling on experimentally induced rat 
      periapical lesions.
PG  - 227-244
LID - 10.1111/iej.13861 [doi]
AB  - AIM: To reveal the molecular mechanisms that targets mitogen-activated protein 
      kinase (MAPK) signalling and the autophagic flux and to investigate the possible 
      effects of the systemic administration of selenium (Se) on experimentally induced 
      rat periapical lesions. METHODOLOGY: Thirty adult Sprague-Dawley rats were 
      divided equally into negative control, positive control and Se groups. In the 
      positive control and Se groups, the pulp chambers of their mandibular first 
      molars were exposed to the oral environment to induce periapical lesions The Se 
      group received daily intraperitoneal injections of Se at a dose of 0.1 mg kg(-1) 
      . After 28 days, the amount of bone destruction; severity of inflammation; 
      penetration of microorganisms along the root canal; collagen degradation in 
      periodontal ligament; interleukin (IL)-6, hypoxia-inducible factor-1 (HIF-1), 
      cyclooxygenase-2 (COX-2) and caspase-3 expression; autophagic flux; and p38 MAPK 
      signalling were evaluated using radiographic, histopathological, Gram staining, 
      picrosirius red stain, immunohistochemical, quantitative real-time polymerase 
      chain (qRT-PCR) and Western blot methods, respectively. These data were analysed 
      through the Kruskal-Wallis and Dunnett's tests (p < .05). RESULTS: The area of 
      radiographic periapical bone loss, histopathological scores, the area of 
      periapical bone loss and the scores for the bacteria localisation, the intensity 
      of immunohistochemical staining for IL-6, HIF-1, COX-2 and caspase-3 in the Se 
      group was significantly less than those of the positive control group (p < .01). 
      The mRNA expression levels of Beclin-1, Atg3, Atg5, Atg7 and Atg16L1 were lower 
      in the Se group than in the positive control group (p < .01). The protein 
      expressions of Beclin-1, Atg5 and LC3-II, the phosphorylation ratio of the p38 
      MAPK and the ratios of LC3II/LC3I were significantly higher (p < .05) in the 
      positive control and Se groups. On the contrary, the expression of the p62/SQSTM1 
      protein was significantly lower (p < .05) in the positive control and Se groups 
      than in the negative control group. CONCLUSION: The induction of periapical 
      lesions in rats increased autophagic flux and activated p38 MAPK signal 
      transduction processes. Se suppressed the inflammatory process, reduced bone 
      destruction and both the autophagic flux and p38 MAPK activation.
CI  - (c) 2022 British Endodontic Society. Published by John Wiley & Sons Ltd.
FAU - Bolat, Nergiz
AU  - Bolat N
AD  - Department of Endodontics, Faculty of Dentistry, Suleyman Demirel University, 
      Isparta, Turkey.
FAU - Erzurumlu, Yalcin
AU  - Erzurumlu Y
AD  - Department of Biochemistry, Faculty of Pharmacy, Suleyman Demirel University, 
      Isparta, Turkey.
FAU - Asci, Halil
AU  - Asci H
AD  - Department of Pharmacology, Faculty of Medicine, Suleyman Demirel University, 
      Isparta, Turkey.
FAU - Ozmen, Ozlem
AU  - Ozmen O
AD  - Department of Pathology, Faculty of Veterinary, Mehmet Akif Ersoy University, 
      Burdur, Turkey.
FAU - Ureyen Kaya, Bulem
AU  - Ureyen Kaya B
AUID- ORCID: 0000-0002-8295-4347
AD  - Department of Endodontics, Faculty of Dentistry, Suleyman Demirel University, 
      Isparta, Turkey.
LA  - eng
GR  - TDH-2020-8040/Department of Scientific Research Projects, Suleyman Demirel 
      University/
PT  - Journal Article
DEP - 20221109
PL  - England
TA  - Int Endod J
JT  - International endodontic journal
JID - 8004996
RN  - H6241UJ22B (Selenium)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 3.4.22.- (Caspase 3)
RN  - 0 (Beclin-1)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
MH  - Rats
MH  - Animals
MH  - Rats, Sprague-Dawley
MH  - *Selenium
MH  - Mitogen-Activated Protein Kinases/metabolism
MH  - Caspase 3/metabolism
MH  - Beclin-1
MH  - Cyclooxygenase 2/metabolism
MH  - Inflammation
MH  - Signal Transduction
MH  - p38 Mitogen-Activated Protein Kinases/metabolism
OTO - NOTNLM
OT  - MAPK
OT  - animal model
OT  - autophagy
OT  - inflammation
OT  - periapical lesion
OT  - selenium
EDAT- 2022/11/01 06:00
MHDA- 2023/01/17 06:00
CRDT- 2022/10/31 05:18
PHST- 2022/10/24 00:00 [revised]
PHST- 2022/03/14 00:00 [received]
PHST- 2022/10/25 00:00 [accepted]
PHST- 2022/11/01 06:00 [pubmed]
PHST- 2023/01/17 06:00 [medline]
PHST- 2022/10/31 05:18 [entrez]
AID - 10.1111/iej.13861 [doi]
PST - ppublish
SO  - Int Endod J. 2023 Feb;56(2):227-244. doi: 10.1111/iej.13861. Epub 2022 Nov 9.