PMID- 36315216
OWN - NLM
STAT- MEDLINE
DCOM- 20221115
LR  - 20221201
IS  - 1557-8976 (Electronic)
IS  - 0882-8245 (Linking)
VI  - 35
IP  - 9
DP  - 2022 Nov
TI  - Role of Autophagy in the Ubiquitinated Hepatitis B Virus Core Antigen Enhancing 
      Dendritic Cell Function.
PG  - 629-639
LID - 10.1089/vim.2022.0062 [doi]
AB  - Dendritic cells (DCs), as the most powerful antigen-presenting cells, play a key 
      role in the adaptive immune response, while the defective function of DC is an 
      important factor in immune tolerance to hepatitis B virus (HBV) infection. 
      Hepatitis B virus core antigen (HBcAg) is a highly antigenic protein that can 
      induce a strong antigen-specific immune response against HBV. In this study, we 
      first constructed the ubiquitinated HBcAg gene (UbHBcAg), and then utilized a 
      recombinant lentiviral vector UbHBcAg (LV-UbHBcAg) to explore the role of them in 
      DC autophagy and function. Meanwhile, the effects of autophagy on DC functional 
      activation were further analyzed. Finally, we investigated the underlying 
      mechanism of autophagy induced by LV-UbHBcAg. Results showed that LV-UbHBcAg 
      could promote autophagic progression in DCs, and the upregulated autophagy can 
      further enhance DC functional maturation. In addition, p62 may serve as an 
      important role in autophagy degradation. More importantly, the PI3K/Akt/mTOR 
      signaling pathway was involved in the process of autophagy induced by LV-UbHBcAg. 
      These findings suggest that LV-UbHBcAg can activate DC function by inducing 
      autophagy, which may represent a promising strategy to treat chronic HBV 
      infection.
FAU - Huang, Run
AU  - Huang R
AUID- ORCID: 0000-0002-2941-6785
AD  - Department of Infectious Disease, Shanghai Sixth People's Hospital Affiliated to 
      Shanghai Jiao Tong University School of Medicine, Shanghai, China.
FAU - Chen, Jie
AU  - Chen J
AD  - Department of Infectious Disease, Shanghai Sixth People's Hospital Affiliated to 
      Shanghai Jiao Tong University School of Medicine, Shanghai, China.
FAU - Tan, Quanhui
AU  - Tan Q
AD  - Department of Infectious Disease, Shanghai Sixth People's Hospital Affiliated to 
      Shanghai Jiao Tong University School of Medicine, Shanghai, China.
FAU - Hu, Weiwei
AU  - Hu W
AD  - Department of Infectious Disease, Shanghai Sixth People's Hospital Affiliated to 
      Shanghai Jiao Tong University School of Medicine, Shanghai, China.
FAU - Chen, Xiaohua
AU  - Chen X
AD  - Department of Infectious Disease, Shanghai Sixth People's Hospital Affiliated to 
      Shanghai Jiao Tong University School of Medicine, Shanghai, China.
FAU - Yu, Yongsheng
AU  - Yu Y
AD  - Department of Infectious Disease, Shanghai Sixth People's Hospital Affiliated to 
      Shanghai Jiao Tong University School of Medicine, Shanghai, China.
FAU - Zang, Guoqing
AU  - Zang G
AD  - Department of Infectious Disease, Shanghai Sixth People's Hospital Affiliated to 
      Shanghai Jiao Tong University School of Medicine, Shanghai, China.
FAU - Tang, Zhenghao
AU  - Tang Z
AD  - Department of Infectious Disease, Shanghai Sixth People's Hospital Affiliated to 
      Shanghai Jiao Tong University School of Medicine, Shanghai, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20221028
PL  - United States
TA  - Viral Immunol
JT  - Viral immunology
JID - 8801552
RN  - 0 (Hepatitis B Core Antigens)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
SB  - IM
MH  - Humans
MH  - *Hepatitis B Core Antigens/genetics
MH  - Hepatitis B virus
MH  - Phosphatidylinositol 3-Kinases/genetics/metabolism/pharmacology
MH  - Genetic Vectors
MH  - Dendritic Cells
MH  - Autophagy
MH  - *Hepatitis B
OTO - NOTNLM
OT  - PI3K/Akt/mTOR signaling pathway
OT  - autophagy
OT  - dendritic cells
OT  - hepatitis B virus core antigen
OT  - ubiquitinated
EDAT- 2022/11/01 06:00
MHDA- 2022/11/16 06:00
CRDT- 2022/10/31 11:52
PHST- 2022/11/01 06:00 [pubmed]
PHST- 2022/11/16 06:00 [medline]
PHST- 2022/10/31 11:52 [entrez]
AID - 10.1089/vim.2022.0062 [doi]
PST - ppublish
SO  - Viral Immunol. 2022 Nov;35(9):629-639. doi: 10.1089/vim.2022.0062. Epub 2022 Oct 
      28.