PMID- 36318091
OWN - NLM
STAT- MEDLINE
DCOM- 20230315
LR  - 20230403
IS  - 1530-8022 (Electronic)
IS  - 0885-3282 (Linking)
VI  - 37
IP  - 8
DP  - 2023 Mar
TI  - Primary hepatocyte urea assessment in the sodium-alginate patterned hydrogel by 
      electrochemical procedure containing umbilical cord conditioned media.
PG  - 1470-1485
LID - 10.1177/08853282221137093 [doi]
AB  - Limitations in liver transplantation and advances in cell therapy methods 
      motivated us to study primary hepatocytes. The main challenge in using primary 
      hepatocytes for liver regeneration is that they lose their functionalities. We 
      aimed to develop a controlled-shape hydrogel and apply the conditioned-media of 
      mesenchymal stromal cells (CM-MSCs) to improve in vitro hepatocyte functions. In 
      this experimental study, following rat hepatocyte isolation by collagenase 
      perfusion and collection of human umbilical cord CM-MSCs, a simple and precise 
      system called electrodeposition was used to produce the patterned alginate 
      hydrogel. To reduce the cytopathic effects, we used an indirect electrodeposition 
      method. For characterizing this structure, mechanical properties, 
      Fourier-transform infrared spectroscopy (FTIR), water uptake, in-vitro 
      degradation, and hydrogel stability were studied. Urea synthesis as a basic 
      function of hepatocytes was assessed in five different groups. Scanning electron 
      microscope (SEM) was utilized to evaluate the primary hepatocyte morphology and 
      their dispersion in the fabricated structure. We observed a significant increase 
      in urea synthesis in the presence of CM-MSCs in patterned hydrogel alginate 
      compared to 2D culture on day 3 (p<0.05). However, there was no significant 
      difference in simple and patterned hydrogel on day 2. We found that the 
      electrodeposition method is appropriate for the rapid fabricating of hydrogel 
      structures with arbitrary patterns for 3D cell culture.
FAU - Abolhassani, Sareh
AU  - Abolhassani S
AUID- ORCID: 0000-0001-9313-243X
AD  - School of Advanced Medical Sciences and Technologies, 48435Shiraz University of 
      Medical Sciences, Shiraz, Iran.
FAU - Hossein-Aghdaei, Mahdokht
AU  - Hossein-Aghdaei M
AD  - Transplant Research Center, 226722Shiraz University of Medical Sciences, Shiraz, 
      Iran.
FAU - Geramizadeh, Bita
AU  - Geramizadeh B
AD  - Transplant Research Center, 226722Shiraz University of Medical Sciences, Shiraz, 
      Iran.
FAU - Azarpira, Negar
AU  - Azarpira N
AD  - Transplant Research Center, 226722Shiraz University of Medical Sciences, Shiraz, 
      Iran.
FAU - Koohpeyma, Farhad
AU  - Koohpeyma F
AD  - Endocrine and metabolism Research Center, 48435Shiraz University of Medical 
      Sciences, Shiraz, Iran.
FAU - Gholami, Mahnaz
AU  - Gholami M
AD  - Transplant Research Center, 226722Shiraz University of Medical Sciences, Shiraz, 
      Iran.
FAU - Alizadeh, Aliakbar
AU  - Alizadeh A
AD  - School of Advanced Medical Sciences and Technologies, 48435Shiraz University of 
      Medical Sciences, Shiraz, Iran.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20221101
PL  - England
TA  - J Biomater Appl
JT  - Journal of biomaterials applications
JID - 8813912
RN  - 0 (Hydrogels)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Alginates)
RN  - 8W8T17847W (Urea)
RN  - 9NEZ333N27 (Sodium)
SB  - IM
MH  - Rats
MH  - Humans
MH  - Animals
MH  - *Hydrogels/metabolism
MH  - Culture Media, Conditioned
MH  - *Alginates/chemistry
MH  - Urea
MH  - Hepatocytes
MH  - Umbilical Cord
MH  - Sodium/metabolism
OTO - NOTNLM
OT  - Ca-alginate hydrogel
OT  - conditioned medium
OT  - electrodeposition
OT  - primary hepatocyte
OT  - regenerative medicine
OT  - urea assessment
EDAT- 2022/11/02 06:00
MHDA- 2023/03/16 06:00
CRDT- 2022/11/01 10:42
PHST- 2022/11/02 06:00 [pubmed]
PHST- 2023/03/16 06:00 [medline]
PHST- 2022/11/01 10:42 [entrez]
AID - 10.1177/08853282221137093 [doi]
PST - ppublish
SO  - J Biomater Appl. 2023 Mar;37(8):1470-1485. doi: 10.1177/08853282221137093. Epub 
      2022 Nov 1.