PMID- 36319592
OWN - NLM
STAT- MEDLINE
DCOM- 20221129
LR  - 20221222
IS  - 1365-2826 (Electronic)
IS  - 0953-8194 (Linking)
VI  - 34
IP  - 11
DP  - 2022 Nov
TI  - Partial loss of arcuate kisspeptin neurons in female rats stimulates luteinizing 
      hormone and decreases prolactin secretion induced by estradiol.
PG  - e13204
LID - 10.1111/jne.13204 [doi]
AB  - Kisspeptin, neurokinin, and dynorphin (KNDy) neurons in the arcuate nucleus (ARC) 
      control luteinizing hormone (LH) and prolactin (PRL) release, although their role 
      in conveying the effects of estradiol (E(2) ) to these hormones is not well 
      understood. We performed a longitudinal evaluation of female rats in which KNDy 
      neurons were ablated using a neurokinin-3 receptor agonist conjugated with 
      saporin (NK3-SAP) to investigate the impact of the reduction of KNDy neurons on 
      the E(2) regulation of gonadal and PRL axes. NK3-SAP rats, bearing a moderate 
      loss of ARC kisspeptin-immunoreactive (-IR) neurons (50%-90%), displayed 
      irregular estrous cycles but essentially unaltered follicular development and a 
      normal number of corpora lutea. Rats were then ovariectomized (OVX) and treated 
      with a positive-feedback dose of E(2) (OVX + E(2) ). LH and PRL were measured in 
      the tail blood by an enzyme-linked immunosorbent assay. The E(2) -induced LH 
      surge was amplified, whereas the PRL rise was decreased in NK3-SAP rats compared 
      to Blank-SAP control. After 10 days of no hormonal treatment, basal LH levels 
      were equally elevated in NK3-SAP and controls. Tyrosine hydroxylase (TH) 
      phosphorylation in the median eminence, in turn, was increased in NK3-SAP rats, 
      with no change in the number of ARC TH-IR neurons. Thus, KNDy neurons exert 
      concurrent and opposite roles in the E(2) -induced surges of LH and PRL. The 
      partial loss of KNDy neurons disrupts ovarian cyclicity but does not preclude 
      ovulation, consistent with the disinhibition of the LH preovulatory surge. 
      Conversely, KNDy neurons tonically inhibit the enzymatic activity of 
      tuberoinfundibular dopaminergic neurons, which appears to facilitate PRL release 
      in response to E(2) .
CI  - (c) 2022 British Society for Neuroendocrinology.
FAU - Campideli-Santana, Ana C
AU  - Campideli-Santana AC
AUID- ORCID: 0000-0001-5225-4940
AD  - Departamento de Fisiologia e Biofisica, Instituto de Ciencias Biologicas, 
      Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
FAU - Gusmao, Daniela O
AU  - Gusmao DO
AD  - Departamento de Fisiologia e Biofisica, Instituto de Ciencias Biologicas, 
      Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
AD  - Departamento de Fisiologia e Biofisica, Instituto de Ciencias Biomedicas, 
      Universidade de Sao Paulo, Sao Paulo, Brazil.
FAU - Almeida, Fernanda R C L
AU  - Almeida FRCL
AD  - Departamento de Morfologia, Instituto de Ciencias Biologicas, Universidade 
      Federal de Minas Gerais, Belo Horizonte, Brazil.
FAU - Araujo-Lopes, Roberta
AU  - Araujo-Lopes R
AD  - Departamento de Fisiologia e Biofisica, Instituto de Ciencias Biologicas, 
      Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
FAU - Szawka, Raphael E
AU  - Szawka RE
AUID- ORCID: 0000-0002-2639-3469
AD  - Departamento de Fisiologia e Biofisica, Instituto de Ciencias Biologicas, 
      Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20221101
PL  - United States
TA  - J Neuroendocrinol
JT  - Journal of neuroendocrinology
JID - 8913461
RN  - 0 (Kisspeptins)
RN  - 9002-62-4 (Prolactin)
RN  - 4TI98Z838E (Estradiol)
RN  - 9002-67-9 (Luteinizing Hormone)
RN  - 74913-18-1 (Dynorphins)
RN  - EC 1.14.16.2 (Tyrosine 3-Monooxygenase)
RN  - 86933-75-7 (Neurokinin B)
SB  - IM
MH  - Female
MH  - Rats
MH  - Animals
MH  - *Kisspeptins/metabolism
MH  - *Prolactin/pharmacology
MH  - Estradiol/pharmacology
MH  - Luteinizing Hormone
MH  - Arcuate Nucleus of Hypothalamus/metabolism
MH  - Dynorphins/metabolism
MH  - Neurons/metabolism
MH  - Tyrosine 3-Monooxygenase
MH  - Neurokinin B/metabolism
OTO - NOTNLM
OT  - KNDy
OT  - dopamine
OT  - luteinizing hormone
OT  - prolactin
OT  - tyrosine hydroxylase
EDAT- 2022/11/02 06:00
MHDA- 2022/11/30 06:00
CRDT- 2022/11/01 23:22
PHST- 2022/08/25 00:00 [revised]
PHST- 2022/05/18 00:00 [received]
PHST- 2022/09/30 00:00 [accepted]
PHST- 2022/11/02 06:00 [pubmed]
PHST- 2022/11/30 06:00 [medline]
PHST- 2022/11/01 23:22 [entrez]
AID - 10.1111/jne.13204 [doi]
PST - ppublish
SO  - J Neuroendocrinol. 2022 Nov;34(11):e13204. doi: 10.1111/jne.13204. Epub 2022 Nov 
      1.