PMID- 36319705
OWN - NLM
STAT- MEDLINE
DCOM- 20221103
LR  - 20221103
IS  - 1573-0972 (Electronic)
IS  - 0959-3993 (Linking)
VI  - 38
IP  - 12
DP  - 2022 Nov 2
TI  - Metabolic adaptation and ATP homeostasis in Pseudomonas fluorescens exposed to 
      phosphate stress.
PG  - 255
LID - 10.1007/s11274-022-03432-z [doi]
AB  - Phosphate (Pi) is essential for life as it is an integral part of the universal 
      chemical energy adenosine triphosphate (ATP), and macromolecules such as, DNA, 
      RNA proteins and lipids. Despite the core roles and the need of this nutrient in 
      living cells, some bacteria can grow in environments that are poor in Pi. The 
      metabolic mechanisms that enable bacteria to proliferate in a low phosphate 
      environment are not fully understood. In this study, the soil microbe Pseudomonas 
      (P.) fluorescens was cultured in a control and a low Pi (stress) medium in order 
      to delineate how energy homeostasis is maintained. Although there was no 
      significant variation in biomass yield in these cultures, metabolites like 
      isocitrate, oxaloacetate, pyruvate and phosphoenolpyruvate (PEP) were markedly 
      increased in the phosphate-starved condition. Components of the glycolytic, 
      glyoxylate and tricarboxylic acid cycles operated in tandem to generate ATP by 
      substrate level phosphorylation (SLP) as NADH-producing enzymes were impeded. The 
      alpha-ketoglutarate (KG) produced when glutamine, the sole carbon nutrient was 
      transformed into phosphoenol pyruvate (PEP) and succinyl-CoA (SC), two high 
      energy moieties. The metabolic reprogramming orchestrated by isocitrate lyase 
      (ICL), phosphoenolpyruvate synthase (PEPS), pyruvate phosphate dikinase (PPDK), 
      and succinyl-CoA synthetase fulfilled the ATP budget. Cell free extract 
      experiments confirmed ATP synthesis in the presence of such substrates as PEP, 
      oxaloacetate and isocitrate respectively. Gene expression profiling revealed 
      elevated transcripts associated with numerous enzymes including ICL, PEPS, and 
      succinyl-CoA synthetase (SCS). This microbial adaptation will be critical in 
      promoting biological activity in Pi-poor ecosystems.
CI  - (c) 2022. The Author(s), under exclusive licence to Springer Nature B.V.
FAU - Legendre, Felix
AU  - Legendre F
AD  - School of Natural Sciences, Laurentian University, P3E 2C6, Sudbury, ON, Canada.
FAU - MacLean, Alex
AU  - MacLean A
AD  - School of Natural Sciences, Laurentian University, P3E 2C6, Sudbury, ON, Canada.
FAU - Tharmalingam, Sujeenthar
AU  - Tharmalingam S
AD  - School of Natural Sciences, Laurentian University, P3E 2C6, Sudbury, ON, Canada.
AD  - Northern Ontario School of Medicine, P3E 2C6, Sudbury, ON, Canada.
FAU - Appanna, Vasu D
AU  - Appanna VD
AD  - School of Natural Sciences, Laurentian University, P3E 2C6, Sudbury, ON, Canada. 
      vappanna@laurentian.ca.
LA  - eng
GR  - CGSD2 - 535659 - 2019/Natural Sciences and Engineering Research Council of 
      Canada/
PT  - Journal Article
DEP - 20221102
PL  - Germany
TA  - World J Microbiol Biotechnol
JT  - World journal of microbiology & biotechnology
JID - 9012472
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - 9RW6G5D4MQ (isocitric acid)
RN  - 0 (Isocitrates)
RN  - 0 (Phosphates)
RN  - 73-89-2 (Phosphoenolpyruvate)
RN  - 8558G7RUTR (Pyruvic Acid)
RN  - 0 (Oxaloacetates)
RN  - EC 6.- (Ligases)
SB  - IM
MH  - *Pseudomonas fluorescens/metabolism
MH  - Adenosine Triphosphate/metabolism
MH  - Isocitrates/metabolism
MH  - Phosphates/metabolism
MH  - Ecosystem
MH  - Phosphoenolpyruvate/metabolism
MH  - Homeostasis
MH  - Pyruvic Acid/metabolism
MH  - Oxaloacetates/metabolism
MH  - Ligases/metabolism
EDAT- 2022/11/03 06:00
MHDA- 2022/11/04 06:00
CRDT- 2022/11/02 00:26
PHST- 2022/06/07 00:00 [received]
PHST- 2022/10/05 00:00 [accepted]
PHST- 2022/11/02 00:26 [entrez]
PHST- 2022/11/03 06:00 [pubmed]
PHST- 2022/11/04 06:00 [medline]
AID - 10.1007/s11274-022-03432-z [pii]
AID - 10.1007/s11274-022-03432-z [doi]
PST - epublish
SO  - World J Microbiol Biotechnol. 2022 Nov 2;38(12):255. doi: 
      10.1007/s11274-022-03432-z.