PMID- 36323342
OWN - NLM
STAT- MEDLINE
DCOM- 20221215
LR  - 20230111
IS  - 1872-7905 (Electronic)
IS  - 0022-1759 (Linking)
VI  - 511
DP  - 2022 Dec
TI  - Differences in mouse strains determine the outcome of Der p 2 allergy induction 
      protocols.
PG  - 113382
LID - S0022-1759(22)00169-7 [pii]
LID - 10.1016/j.jim.2022.113382 [doi]
AB  - In vivo animal models can provide worthy information on various aspects of asthma 
      mechanism and pathogenesis. The genetic predisposition and phenotype of mice may 
      affect the immune response itself. Here we compare the early immune response to 
      Der p 2 or HDM allergen extract upon injection and inhalation in BALB/c and 
      C57BL/6 mice. Female C57BL/6 and BALB/c mice were immunized with Der p 2 allergen 
      subcutaneously followed by inhalation of Der p 2 or HDM extract. After challenge, 
      the mice were euthanized; blood, bronchoalveolar lavage (BAL), spleens and lungs 
      were collected. Cells from BAL were identified by May-Grunwald Giemsa staining 
      and lung leukocyte populations were analyzed by flow cytometry. Serum antibody 
      levels of Der p 2 specific IgE, IgG, IgG1 and IgG2a were assessed by ELISA, and 
      cytokine secretion (IL-4, IFN-gamma and IL-10) was evaluated upon stimulation with 
      Der p 2 or HDM extract. The Th2 immune response was confirmed by elevated 
      allergen-specific immunoglobulin E (IgE) and the allergic reaction was evidenced 
      by infiltration of eosinophils and/or neutrophils into BAL. We found that BALB/c 
      mice were inefficient in integrating local with systemic immune response, 
      evidenced by almost no IgG or IgE production upon one subcutaneous injection and 
      subsequent inhalation of Der p 2 allergen; also, the bronchoalveolar lavage 
      infiltrate in these mice consisted of neutrophil infiltration, unlike C57BL/6 
      mice in which eosinophilic infiltrate predominated. The differences between 
      BALB/c and C57BL/6 mice strains could be exploited for generating different types 
      of responses to the Der p 2 allergen.
CI  - Copyright (c) 2022 Elsevier B.V. All rights reserved.
FAU - Lopandic, Zorana
AU  - Lopandic Z
AD  - Department of Biochemistry, Faculty of Chemistry, University of Belgrade, 
      Studentski trg 16, 11000 Belgrade, Serbia.
FAU - Dragacevic, Luka
AU  - Dragacevic L
AD  - Institute of Virology, Vaccines and Sera, Torlak, Vojvode Stepe 458, 11152 
      Belgrade, Serbia.
FAU - Kosanovic, Dejana
AU  - Kosanovic D
AD  - Institute of Virology, Vaccines and Sera, Torlak, Vojvode Stepe 458, 11152 
      Belgrade, Serbia.
FAU - Burazer, Lidija
AU  - Burazer L
AD  - Institute of Virology, Vaccines and Sera, Torlak, Vojvode Stepe 458, 11152 
      Belgrade, Serbia.
FAU - Gavrovic-Jankulovic, Marija
AU  - Gavrovic-Jankulovic M
AD  - Department of Biochemistry, Faculty of Chemistry, University of Belgrade, 
      Studentski trg 16, 11000 Belgrade, Serbia.
FAU - Minic, Rajna
AU  - Minic R
AD  - Institute of Virology, Vaccines and Sera, Torlak, Vojvode Stepe 458, 11152 
      Belgrade, Serbia; Group for Immunology, Institute for Medical Research, National 
      Institute of Republic of Serbia, University of Belgrade, dr Subotica 4, Belgrade, 
      Serbia. Electronic address: rajna.minic@imi.bg.ac.rs.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20221030
PL  - Netherlands
TA  - J Immunol Methods
JT  - Journal of immunological methods
JID - 1305440
RN  - 0 (Allergens)
SB  - IM
MH  - Female
MH  - Mice
MH  - Animals
MH  - Mice, Inbred C57BL
MH  - Neutrophil Infiltration
MH  - *Hypersensitivity
MH  - Allergens
OTO - NOTNLM
OT  - Allergy
OT  - Asthma
OT  - BALB/c
OT  - C57BL/6
OT  - House dust mite
OT  - Mouse model
COIS- Declaration of Competing Interest The Authors declare that there is no conflict 
      of interest.
EDAT- 2022/11/03 06:00
MHDA- 2022/12/15 06:00
CRDT- 2022/11/02 20:23
PHST- 2022/08/03 00:00 [received]
PHST- 2022/10/21 00:00 [revised]
PHST- 2022/10/24 00:00 [accepted]
PHST- 2022/11/03 06:00 [pubmed]
PHST- 2022/12/15 06:00 [medline]
PHST- 2022/11/02 20:23 [entrez]
AID - S0022-1759(22)00169-7 [pii]
AID - 10.1016/j.jim.2022.113382 [doi]
PST - ppublish
SO  - J Immunol Methods. 2022 Dec;511:113382. doi: 10.1016/j.jim.2022.113382. Epub 2022 
      Oct 30.