PMID- 36323362
OWN - NLM
STAT- MEDLINE
DCOM- 20221129
LR  - 20221129
IS  - 1879-0038 (Electronic)
IS  - 0378-1119 (Linking)
VI  - 851
DP  - 2023 Jan 30
TI  - Transcriptome analysis of hepatopancreas and gills of Palaemon gravieri under 
      salinity stress.
PG  - 147013
LID - S0378-1119(22)00833-2 [pii]
LID - 10.1016/j.gene.2022.147013 [doi]
AB  - Salinity is an important factor in the aquatic environment, and its fluctuations 
      always result in osmotic stress, which affects the survival, distribution, and 
      physiological activities of crustaceans. Crustaceans counter them through 
      osmoregulation, which consists of many mechanisms. Palaemon gravieri is an 
      important economic species in Palaemonidae, widely distributed in the southern 
      East China Sea and the China Yellow Sea, and has a good adaptability to salinity 
      stress. Currently, there are only a few studies on the effects of salinity on P. 
      graviera. Therefore, it is particularly important to study the molecular 
      responses of P. gravieri to salinity fluctuations. In this study, P. gravieri was 
      treated with salinities of 10, 25, and 40, and the hepatopancreas and gills of 
      shrimp in the different salinity groups were sampled after 24 h. The samples were 
      used for RNA extraction and transcriptome analysis. In total, 80,994 unigenes 
      were obtained, of which 19,114 were annotated. The differences in gene expression 
      between different tissues at the same salinity were more significant. Many 
      metabolism-related genes were downregulated in the gills, such as 
      beta-hexosaminidase subunit alpha (HEXA), 10-formyltetrahydrofolate dehydrogenase 
      (ALDH1L1), and Alcohol dehydrogenase class-3 (ADH5). Scanning transmission 
      electron microscope analysis showed that the expression levels of some 
      stress-(but not salinity stress) related genes changed after stress (mostly 
      upregulated), suggesting the existence of secondary stress. Gene set enrichment 
      analysis (GSEA) focused on the expression of transporters in osmoregulation, and 
      the results showed that they mainly played a role in the gills, but ATP-binding 
      cassette (ABC) transporters were more active in the hepatopancreas. This study 
      showed that the response of P. gravieri to salinity change was different not only 
      between the hepatopancreas and gills, but also between low salinity and higher 
      salinity, and the ion transport-related genes were mainly expressed in the gills. 
      Overall, these results improve our understanding of salt tolerance mechanism in 
      P. gravieri.
CI  - Copyright (c) 2022. Published by Elsevier B.V.
FAU - Shi, Wenjun
AU  - Shi W
AD  - Institute of Oceanology & Marine Fisheries, Jiangsu, Nantong 226007, China; 
      Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 
      264003, China.
FAU - Hu, Runhao
AU  - Hu R
AD  - Institute of Oceanology & Marine Fisheries, Jiangsu, Nantong 226007, China; 
      National Demonstration Center for Experimental Fisheries Science Education, 
      Shanghai Ocean University, Shanghai 201306, China.
FAU - Zhao, Ran
AU  - Zhao R
AD  - Institute of Oceanology & Marine Fisheries, Jiangsu, Nantong 226007, China; 
      National Demonstration Center for Experimental Fisheries Science Education, 
      Shanghai Ocean University, Shanghai 201306, China.
FAU - Zhu, Jianqiang
AU  - Zhu J
AD  - Institute of Oceanology & Marine Fisheries, Jiangsu, Nantong 226007, China; 
      National Demonstration Center for Experimental Fisheries Science Education, 
      Shanghai Ocean University, Shanghai 201306, China.
FAU - Shen, Hui
AU  - Shen H
AD  - Institute of Oceanology & Marine Fisheries, Jiangsu, Nantong 226007, China.
FAU - Li, Hui
AU  - Li H
AD  - Institute of Oceanology & Marine Fisheries, Jiangsu, Nantong 226007, China.
FAU - Wang, Libao
AU  - Wang L
AD  - Institute of Oceanology & Marine Fisheries, Jiangsu, Nantong 226007, China.
FAU - Yang, Zeyu
AU  - Yang Z
AD  - Aquatic Product Technology Extension Station of Gaoyou, Gaoyou 225634, China.
FAU - Jiang, Qi
AU  - Jiang Q
AD  - Institute of Oceanology & Marine Fisheries, Jiangsu, Nantong 226007, China.
FAU - Qiao, Yi
AU  - Qiao Y
AD  - Institute of Oceanology & Marine Fisheries, Jiangsu, Nantong 226007, China.
FAU - Jiang, Ge
AU  - Jiang G
AD  - Institute of Oceanology & Marine Fisheries, Jiangsu, Nantong 226007, China.
FAU - Cheng, Jie
AU  - Cheng J
AD  - Institute of Oceanology & Marine Fisheries, Jiangsu, Nantong 226007, China.
FAU - Wan, Xihe
AU  - Wan X
AD  - Institute of Oceanology & Marine Fisheries, Jiangsu, Nantong 226007, China. 
      Electronic address: wxh1708@163.com.
LA  - eng
PT  - Journal Article
DEP - 20221030
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
SB  - IM
MH  - Animals
MH  - *Hepatopancreas/metabolism
MH  - Gills/metabolism
MH  - *Palaemonidae/genetics
MH  - Gene Expression Profiling
MH  - Osmoregulation/genetics
MH  - Transcriptome
OTO - NOTNLM
OT  - Metabolism
OT  - Palaemon gravieri
OT  - Salinity stress
OT  - Transcriptome
OT  - Transporters
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2022/11/03 06:00
MHDA- 2022/11/30 06:00
CRDT- 2022/11/02 20:24
PHST- 2022/04/04 00:00 [received]
PHST- 2022/09/01 00:00 [revised]
PHST- 2022/10/25 00:00 [accepted]
PHST- 2022/11/03 06:00 [pubmed]
PHST- 2022/11/30 06:00 [medline]
PHST- 2022/11/02 20:24 [entrez]
AID - S0378-1119(22)00833-2 [pii]
AID - 10.1016/j.gene.2022.147013 [doi]
PST - ppublish
SO  - Gene. 2023 Jan 30;851:147013. doi: 10.1016/j.gene.2022.147013. Epub 2022 Oct 30.